Mutational Analysis of Plasmodium vivax dhfr Gene Among Cases in South East of Iran

Rafee, Maryam; Zaman, Jalal; Mehravaran, Ahmad; Salehi, Reza

doi:10.5812/jjm.57697

Cited by 1 publication

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References 31 publications

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“…One of the serious barriers in the way of developing vaccine against malaria is the high genetic diversity ( 16 ). In a study in Mali, provided vaccine was effective against one species of P.falciparum only ( 17 ), and in another study, the bivalent vaccine provided no protection ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

Sequence Analysis of Pvama-1 among Plasmodium Vivax Isolates in Sistan-Baluchistan

Metanat¹,

Tabatabaei²,

Mehravaran³

et al. 2020

Ethiop J Health Sci

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BACKGROUND: Apical Membrane antigen 1 (AMA-1) is an important membrane protein that presents in all Plasmodium species and participates in critical phases in the attraction of cells. In human, it is one of the most immunodominant antigens with a protective immune response simulation role Apical Membrane antigen 1 (AMA-1) is an important membrane protein which presents in all Plasmodium species and is located on the surface of merozoite and sporozoites that participates in critical phases in attraction of human red blood cells by merozoites and hepatocytes by sporozoites, so in human, it is one of the most immunodominant antigens with a protective immune response simulation role. Since extra information is necessary to lighten of AMA-1 scope, we equaled genetic variation in P.vivax AMA-1 from 40 Iranian isolates with those reported from the other malarious countries.METHODS: Blood samples were collected from 40 patients’ positive of P.vivax, and genomic DNA was extracted from the blood. The nucleotide sequence for 446 amino acid (AA) residues (42–488 of PvAMA-1) of AMA-1 gene was amplified via PCR and then sequenced.RESULTS: A total of 24 different haplotypes were recognized between samples. No new haplotype was determined in this research that was reported previously in other regions of Iran and the world. We detected 37-point mutations at the nucleotide level in their sequences and showed 43 amino acid variations, at 37 positions in which 6 sites demonstrate trimorphic polymorphism, and the others were dimorphic.CONCLUSION: Sequence analysis of the major haplotype showed 95% similarity with P.vivax Sal-1 AMA-1 gene and high level of allelic diversity at the domain I of PvAMA-1 among P. vivaxisolates of Iran. Because PvAMA-1 is noticeable as vaccinecandidate antigen, these documents provide valuable informationfor the development of malaria vaccine.

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Section: Introductionmentioning

confidence: 99%

Sequence Analysis of Pvama-1 among Plasmodium Vivax Isolates in Sistan-Baluchistan

Metanat¹,

Tabatabaei²,

Mehravaran³

et al. 2020

Ethiop J Health Sci

View full text Add to dashboard Cite

show abstract

Mutational Analysis of Plasmodium vivax dhfr Gene Among Cases in South East of Iran

Abstract: Background: Plasmodium vivax and P. falciparum have been detected in south east of Iran. Plasmodium vivax has a higher prevalence in this area. Point mutation in P. vivax dihydrofolate reductase (pvdhfr) gene is the key mechanism of Sulfadoxine and Pyrimethamine (SP) resistance.

Cited by 1 publication

References 31 publications

Sequence Analysis of Pvama-1 among Plasmodium Vivax Isolates in Sistan-Baluchistan

Sequence Analysis of Pvama-1 among Plasmodium Vivax Isolates in Sistan-Baluchistan

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