Mutational analysis of foot and mouth disease virus nonstructural polyprotein 3AB-coding region to design a negative marker virus

Bhatt, Mukesh; Mohapatra, Jajati K.; Pandey, Laxmi; Mohanty, Nihar Nalini; Das, Biswajit; Prusty, Bikash Ranjan; Pattnaik, Bramhadev

doi:10.1016/j.virusres.2017.10.010

Cited by 12 publications

(8 citation statements)

References 42 publications

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“…Various groups have developed inactivated vaccines with a modified virus to overcome the limitations of wild type virus inactivated vaccines, such as the lack of foolproof DIVA and the need for a biosafety level III facility [52]. These vaccines provide DIVA compatibility without the need to purify non-structural proteins [53].…”

Section: Trends and Advances In Vaccines Against Fmdvmentioning

confidence: 99%

Foot-and-Mouth Disease Virus: Immunobiology, Advances in Vaccines and Vaccination Strategies Addressing Vaccine Failures—An Indian Perspective

et al. 2019

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A mass vaccination campaign in India seeks to control and eventually eradicate foot-and-mouth disease (FMD). Biosanitary measures along with FMD monitoring are being conducted along with vaccination. The implementation of the FMD control program has drastically reduced the incidence of FMD. However, cases are still reported, even in regions where vaccination is carried out regularly. Control of FMD outbreaks is difficult when the virus remains in circulation in the vaccinated population. Various FMD risk factors have been identified that are responsible for FMD in vaccinated areas. The factors are discussed along with strategies to address these challenges. The current chemically inactivated trivalent vaccine formulation containing strains of serotype O, A, and Asia 1 has limitations including thermolability and induction of only short-term immunity. Advantages and disadvantages of several new-generation alternate vaccine formulations are discussed. It is unfeasible to study every incidence of FMD in vaccinated animals/areas in such a big country as India with its huge livestock population. However, at the same time, it is absolutely necessary to identify the precise reason for vaccination failure. Failure to vaccinate is one reason for the occurrence of FMD in vaccinated areas. FMD epidemiology, emerging and re-emerging virus strains, and serological status over the past 10 years are discussed to understand the impact of vaccination and incidences of vaccination failure in India. Other factors that are important in vaccination failure that we discuss include disrupted herd immunity, health status of animals, FMD carrier status, and FMD prevalence in other species. Recommendations to boost the search of alternate vaccine formulation, strengthen the veterinary infrastructure, bolster the real-time monitoring of FMD, as well as a detailed investigation and documentation of every case of vaccination failure are provided with the goal of refining the control program.

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Section: Trends and Advances In Vaccines Against Fmdvmentioning

confidence: 99%

Foot-and-Mouth Disease Virus: Immunobiology, Advances in Vaccines and Vaccination Strategies Addressing Vaccine Failures—An Indian Perspective

et al. 2019

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“…Since the 2C protein of picornaviruses are highly conserved ( Gorbalenya et al, 1989 ), it is assumed that FMDV 2C should possess most of these activities involved in immune evasion. The FMDV 3A protein is a multifunctional non-structural protein in viral replication, virulence and host-specific genetic features ( Mason et al, 2003 ; Ma X. et al, 2016 ; Bhatt et al, 2017 ; Bohórquez et al, 2017 ; Lotufo et al, 2017 ). For the role of FMDV 3A in the antiviral immune response, when 3A gene was over-expressed in the PK-15 cell line (swine) and the HEK293 cell line (human), 3A protein can disrupt the transcriptional levels involved in RIG-I, MDA5, and MAVS and block the interaction between RIG-I/MDA5 and MAVS via its N-terminal region ( Li et al, 2016d ).…”

Section: Evasion Of Antiviral Response Of Type I Ifn Mediated By Fmdvmentioning

confidence: 99%

Type I Interferon Induced and Antagonized by Foot-and-Mouth Disease Virus

Chang

et al. 2018

Front. Microbiol.

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Viral infections trigger the innate immune system, serving as the first line of defense, and are characterized by the production of type I interferon (IFN). Type I IFN is expressed in a broad spectrum of cells and tissues in the host and includes various subtypes (IFN-α, IFN-β, IFN-δ, IFN-ε, IFN-κ, IFN-τ, IFN-ω, IFN-ν, and IFN-ζ). Since the discovery of type I IFN, our knowledge of the biology of type I IFN has accumulated immensely, and we now have a substantial amount of information on the molecular mechanisms of the response and induction of type I IFN, as well as the strategies utilized by viruses to evade the type I IFN response. Foot-and-mouth disease virus (FMDV) can selectively alter cellular pathways to promote viral replication and evade antiviral immune activation of type I IFN. RNA molecules generated by FMDV are sensed by the cellular receptor for pathogen-associated molecular patterns (PAMPs). FMDV preferentially activates different sensor molecules and various signal transduction pathways. Based on knowledge of the virus or RNA pathogen specificity as well as the function-structure relationship of RNA sensing, it is necessary to summarize numerous signaling adaptors that are reported to participate in the regulation of IFN gene activation.

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“…Fetal porcine kidney (LFBK-α V β 6 ) cells, which have been engineered to express bovine α V β 6 integrin, a principal cellular receptor of FMDV, and fetal goat tongue cells (ZZ-R 127) are two continuous cell lines that are highly sensitive to FMDV (10)(11)(12). A number of studies have utilized the LFBK-α V β 6 and ZZ-R 127 cell lines for the isolation of FMDV from different clinical samples (13)(14)(15)(16)(17)(18)(19)(20). In previous studies, the ZZ-R 127 cell line provided similar sensitivity to FMDV as primary BTY cells (10) and LFBK-α V β 6 cells (21), however to our knowledge the LFBK-α V β 6 cell line has not been compared to BTY cells.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease

Gray¹,

Wood²,

Henry³

et al. 2020

Front. Vet. Sci.

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The most sensitive cell culture system for the isolation of foot-and-mouth disease virus (FMDV) is primary bovine thyroid (BTY) cells. However, BTY cells are seldom used because of the challenges associated with sourcing thyroids from FMDV-negative calves (particularly in FMD endemic countries), and the costs and time required to regularly prepare batches of cells. Two continuous cell lines, a fetal goat tongue cell line (ZZ-R 127) and a fetal porcine kidney cell line (LFBK-α V β 6), have been shown to be highly sensitive to FMDV. Here, we assessed the sensitivity of ZZ-R 127 and LFBK-α V β 6 cells relative to primary BTY cells by titrating a range of FMDV original samples and isolates. Both the ZZ-R 127 and LFBK-α V β 6 cells were susceptible to FMDV for >100 passages, and there were no significant differences in sensitivity relative to primary BTY cells. Notably, the LFBK-α V β 6 cell line was highly sensitive to the O/CATHAY porcine-adapted FMDV strain. These results support the use of ZZ-R 127 and LFBK-α V β 6 as sensitive alternatives to BTY cells for the isolation of FMDV, and highlight the use of LFBK-α V β 6 cells as an additional tool for the isolation of porcinophilic viruses.

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Mutational analysis of foot and mouth disease virus nonstructural polyprotein 3AB-coding region to design a negative marker virus

Cited by 12 publications

References 42 publications

Foot-and-Mouth Disease Virus: Immunobiology, Advances in Vaccines and Vaccination Strategies Addressing Vaccine Failures—An Indian Perspective

Foot-and-Mouth Disease Virus: Immunobiology, Advances in Vaccines and Vaccination Strategies Addressing Vaccine Failures—An Indian Perspective

Type I Interferon Induced and Antagonized by Foot-and-Mouth Disease Virus

Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease

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