Mutational analysis of a baculovirus major late promoter

Morris, Timothy D.; Miller, Lois K.

doi:10.1016/0378-1119(94)90538-x

Cited by 73 publications

(50 citation statements)

References 27 publications

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“…For proper late gene expression of large dsDNA viruses, the motif that contains the TIS is often an important viral promoter element. Mutations in the late motif TAAG of baculoviruses (Morris & Miller, 1994), the late TAAAT motif of poxviruses (Davison & Moss, 1989), the TATA initiator of ASFV (Garcia-Escudero & Viñuela, 2000) and the more diverged initiator YYANWYY (N=any nucleotide; W=t/a) of herpesviruses (Kim et al, 2002;Weir, 2001) often lead to a (dramatic) downregulation of gene expression. The 59 RACE analyses indicate that transcription of the WSSV genes vp28, vp26, vp24, vp19 and vp15 did not start at a consensus sequence for all five genes (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Immediate early (IE) and early (E) genes are expressed before viral DNA replication, while expression of late (L) genes occurs after replication of the viral genome. For proper late gene expression, the motif that contains the transcription initiation site (TIS) often plays a prominent role in recognition by a virus-coded RNA polymerase (Davison & Moss, 1989;Garcia-Escudero & Viñuela, 2000;Kim et al, 2002;Morris & Miller, 1994;Weir, 2001). Information about pathogenicity, epidemiology, virion structure and the genomic sequence of WSSV is now available, but insight into gene regulation is limited.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional analysis of the white spot syndrome virus major virion protein genes

Marks

Mennens

Vlak

et al. 2003

Journal of General Virology

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White spot syndrome virus (WSSV) is a member of a new virus family (Nimaviridae) infecting crustaceans. The regulation of transcription of WSSV genes is largely unknown. Transcription of the major WSSV structural virion protein genes, vp28, vp26, vp24, vp19 and vp15, was studied to search for common promoter motifs for coordinate expression. The temporal expression of these genes and both 59 and 39 ends of the mRNA were determined, using infected crayfish gill tissue as a RNA source. RT-PCR showed that all five genes are expressed late in infection compared to the early ribonucleotide reductase large subunit gene. 59 RACE studies revealed a consensus late transcription initiation motif for only two of the five major virion protein genes. This motif was only found in one other upstream region of the putative translational start site of a gene with unknown function (ORF 158). No other conserved sequence motifs could be detected in the sequences surrounding the transcriptional start sites of the five major virion protein genes. All 59 ends were located about 25 nt downstream of an A/T rich sequence, including the consensus TATA-box sequence for vp15. The absence of a consensus motif is distinct from gene regulation of other large dsDNA viruses and suggests a unique regulation of WSSV transcription, in line with its unique taxonomic position.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of the white spot syndrome virus major virion protein genes

Marks

Mennens

Vlak

et al. 2003

Journal of General Virology

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“…Similarly, an additional 9 or 10 LEF proteins are necessary to reconstitute late transcription and therefore appear to comprise the baculovirus late RNA polymerase and regulatory proteins (19,(23)(24)(25)(26)(27). The viral RNA polymerase recognizes what appears to be an invariant late promoter motif, 5=-TAAG-3= (2,9,(28)(29)(30). In many studies in which late transcription initiation sites were mapped, the mapped initiation sites were variable but were typically found within and very near the TAAG motif.…”

mentioning

confidence: 99%

The Transcriptome of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Cells

Chen

Zhong

Fei

et al. 2013

J Virol

164

225

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“…Most, if not all, late gene promoters contain the conserved sequence 5Ј-TAAG-3Ј (33) at the late transcription start site. The conserved core TAAG sequence plus nonconserved flanking sequences of at least 8 to 12 nucleotides (nt) appear to comprise the late promoter sequences that are necessary for wild-type levels of late transcription (11,24,31). Except for the conserved core TAAG motif, little is known regarding the sequence specificity requirements for late promoter recognition and activation.…”

mentioning

confidence: 99%

Analysis of an Autographa californica Nucleopolyhedrovirus lef-11 Knockout: LEF-11 Is Essential for Viral DNA Replication

Lin

Blissard

2002

J Virol

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The Autographa californica nucleopolyhedrovirus (AcMNPV) lef-11 gene was previously identified by transient late expression assays as a gene important for viral late gene expression. The lef-11 gene was not previously identified as necessary for DNA replication in transient origin-dependent plasmid DNA replication assays. To examine the role of lef-11 in the context of the infection cycle, we generated a deletion of the lef-11 gene by recombination in an AcMNPV genome propagated as a BACmid in Escherichia coli. The resulting AcMNPV lef-11-null BACmid (vAc lef11KO ) was unable to propagate in cell culture, although a "repair" AcMNPV BACmid (vAc lef11KO-REP ), which was generated by transposition of the lef-11 gene into the polyhedrin locus of the vAc lef11KO BACmid, was able to replicate in a manner similar to wild-type or control AcMNPV viruses. Thus, the lef-11 gene is essential for viral replication in Sf9 cells. The vAc lef11KO BACmid was examined to determine if the defect in viral replication resulted from a defect in DNA replication or from a defect in late transcription. The lef-11-null BACmid and control BACmids were transfected into Sf9 cells, and viral DNA replication was monitored. The viral DNA genome of the lef-11-null BACmid (vAc lef11KO ) was not amplified, whereas replication and amplification of the genomes of the repair BACmid (vAc lef11KO-REP ), wild-type AcMNPV, and a nonpropagating gp64-null control BACmid (vAc GUSgp64KO ) were readily detected. Northern blot analysis of transcripts from selected early, late, and very late genes showed that late and very late transcription was absent in cells transfected with the lef-11-null BACmid. Thus, in contrast to prior studies using transient replication and late expression assays, studies of a lef-11-null BACmid indicate that LEF-11 is required for viral DNA replication during the infection cycle.

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Mutational analysis of a baculovirus major late promoter

Cited by 73 publications

References 27 publications

Transcriptional analysis of the white spot syndrome virus major virion protein genes

Transcriptional analysis of the white spot syndrome virus major virion protein genes

The Transcriptome of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Cells

Analysis of an Autographa californica Nucleopolyhedrovirus lef-11 Knockout: LEF-11 Is Essential for Viral DNA Replication

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