Mutation to Increase Sporulation of a Strain of<i>Hirsutella citriformis</i>from Mexico and Evaluation against<i>Diaphorina citri</i>

Cruz-Juárez, Georgina; Maldonado-Blanco, María Guadalupe; Rodríguez-Guerra, Raúl; Torre-Zavala, Susana De la; Avilés-Arnaut, Hamlet; Flores-González, María del Socorro

doi:10.3958/059.043.0407

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…It had high pathogenicity against both nymphs and adults of D. citri. Its pathogenicity against D. citri was comparable to that of Cordyceps javanica, Hirsutella citriformis and C. fumosorosea isolated from D. citri, and the growth rate and spore yield are higher than those of C. javanica [20,21,65]. This is similar to the control effect of azadirachtin and other commonly used pesticides on D. citris [66], so it has the potential to be developed into a new biopesticide resource.…”

Section: Discussionmentioning

confidence: 67%

First Record of Aspergillus fijiensis as an Entomopathogenic Fungus against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Yan

Líu

Idrees

et al. 2022

JoF

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The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the most widespread and devastating pest species in citrus orchards and is the natural vector of the phloem-limited bacterium that causes Huanglongbing (HLB) disease. Thus, reducing the population of D. citri is an important means to prevent the spread of HLB disease. Due to the long-term use of chemical control, biological control has become the most promising strategy. In this study, a novel highly pathogenic fungal strain was isolated from naturally infected cadavers of adult D. citri. The species was identified as Aspergillus fijiensis using morphological identification and phylogenetic analysis and assigned the strain name GDIZM-1. Tests to detect aflatoxin B1 demonstrated that A. fijiensis GDIZM-1 is a non-aflatoxin B1 producer. The pathogenicity of the strain against D. citri was determined under laboratory and greenhouse conditions. The results of the laboratory study indicated that nymphs from the 1st to 5th instar and adults of D. citri were infected by A. fijiensis GDIZM-1. The mortality of nymphs and adults of D. citri caused by infection with A. fijiensis increased with the concentration of the conidial suspension and exposure time, and the median lethal concentration (LC50) and median lethal time (LT50) values gradually decreased. The mortality of D. citri for all instars was higher than 70%, with high pathogenicity at the 7th day post treatment with 1×108 conidia/mL. The results of the greenhouse pathogenicity tests showed that the survival of D. citri adults was 3.33% on the 14th day post-treatment with 1 × 108 conidia/mL, which was significantly lower than that after treatment with the Metarhizium anisopliae GDIZMMa-3 strain and sterile water. The results of the present study revealed that the isolate of A. fijiensis GDIZM-1 was effective against D. citri and it provides a basis for the development of a new microbial pesticide against D. citri after validation of these results in the field.

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Section: Discussionmentioning

confidence: 67%

First Record of Aspergillus fijiensis as an Entomopathogenic Fungus against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Yan

Líu

Idrees

et al. 2022

JoF

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“…evaluated. Under laboratory conditions,Cruz-Juárez et al (2018) exposed an isolate of H. citriformis to different concentrations of ethyl methane sulfonate to obtain mutants with better production and germination of conidia, aiming to maintain virulence against D. citri. The mortality rate achieved by mutant and wild-type isolates was 100 and 94.4%, respectively, within 12 days.…”

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confidence: 99%

Use and perspective of entomopathogenic fungi biocontrol agents of Asian citrus psyllid (Diaphorina citri): A bibliometric review

Portes,

Pimentel,

Zawadneak

2023

RSD

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The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is the main insect vector of the bacteria Candidatus liberibacter spp., which are the causative agents of Citrus Greening disease, also known as Huanglongbing (HLB). The current lack of treatment for the disease means that intensive work is required to manage psyllid populations, mainly through chemical control methods. Since biological control has become a promising management strategy and entomopathogenic fungi are one of the components of integrated pest management, this review aimed to compare previously published results regarding the most efficient fungi for controlling D. citri and to gather information about the best options available. To this end, the bibliography indexed in the Scopus and Web of Science databases was analyzed using bibliometric tools with the R Studio software and the Bibliometrix package. In recent years, Cordyceps fumosorosea, Beauveria bassiana, and Hirsutella citriformis were identified as the most relevant fungus species, showing efficient results. In addition, the countries that publish the most on this subject are also among the largest citrus producers. Studies performed under greenhouse and field conditions are still scarce but necessary to validate the results obtained in the laboratory, as well as those that explore the mechanisms of action involved in the pathogenicity of fungi against D. citri, which are matters to consider in future assessments.

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Effect of Native Strains of Hirsutella citriformis1 on Diaphorina citri2 Adults under Field Conditions

Pérez-González

Arredondo-Bernal

Montesinos-Matías

et al. 2020

Southwestern Entomologist

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Mutation to Increase Sporulation of a Strain ofHirsutella citriformisfrom Mexico and Evaluation againstDiaphorina citri

Cited by 3 publications

References 23 publications

First Record of Aspergillus fijiensis as an Entomopathogenic Fungus against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)

First Record of Aspergillus fijiensis as an Entomopathogenic Fungus against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Use and perspective of entomopathogenic fungi biocontrol agents of Asian citrus psyllid (Diaphorina citri): A bibliometric review

Effect of Native Strains of Hirsutella citriformis1 on Diaphorina citri2 Adults under Field Conditions

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