Mutation research in peas

Heringa, R. J.

doi:10.1007/bf00023113

Cited by 5 publications

(1 citation statement)

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“…Like that root length, shoot length and number of branches were decreased with increase in concentrations of EMS. Similar results from earlier study of Heringa (1964). He reported that 50% reduction in germination at the doses of 40mM EMS.…”

Section: Germinatsupporting

confidence: 89%

Impact of ethyl methane sulphonate on M1 attributes of Groundnut with special emphasis of amino acid profiling

Muniappan¹,

Palanivel²,

Parvathi³

2016

JOAASR

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The present investigation was undertaken to know the effect of EMS on groundnut cultivar TMV-7 particularly for M1 generation with special reference to amino acids. The healthy and viable seeds of groundnut cultivar TMV-7 were exposed to different concentrations of EMS. The chemical mutagens were screened against groundnut ranged from 10 to 50mM and sown in the field along with control. The various growth, morphological parameters like percentage of seed germination, survival percentage plant height, number of branches, days taken for flower initiation , total height at time of harvest, root length, number of lateral roots, number of pods per plant and 100 seeds weight were decreased with increasing concentrations of EMS. The amino acid analysis also carried out in control, EMS derived M1 plants grown in field. The amino acid contents were recorded in both positive and negative tendency.

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Section: Germinatsupporting

confidence: 89%

Impact of ethyl methane sulphonate on M1 attributes of Groundnut with special emphasis of amino acid profiling

Muniappan¹,

Palanivel²,

Parvathi³

2016

JOAASR

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Mutations in peas induced by diethylsulphate and X-rays

Monti¹

1968

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Gibberellic Acid Protection against EMS and MMS Induced Damage to Chromosomes in <i>Pisum sativum</i> L

Narsinghani¹,

Kumar²

1976

CYTOLOGIA

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Chromosomal aberrations produced by physical and chemical mutagens are the main causes of damage in mutation studies However, recent experiments have amply revealed that a great many factors modify the chromosomal damage by affecting the biological changes induced by physical and chemical mutagens (Curtis et al., 1958, Nilan et al., 1961and Konzak et al., 1965. Hormones, e.g., indole acetic acid, along with other chemicals have been found to modify the mutagenic damage only to the extent that they influence the growth pattern in plants (Guttman and Brown 1959, Gaur and Notani 1963and Arartjan 1967.The present experiments relate to cytological changes observed in ethyl methane sulphonate (EMS) and methyl methane sulphonate (MMS) treated peas during M,, M2 and M3 generations. Further, studies were also conducted to study the modify ing effect of post-mutagenic treatments with gibberellic acid. Materials and methodsPea seeds (Pisum sativum L., 2n=14) of variety Bonnvile were used for the present studies. Two chemical mutagens namely ethyl methane sulphonate (EMS) and methyl methane sulphonate (MMS) were employed, while gibberellic acid (GA) was the only hormone tested as a post treatment during these studies. In the first instance, preliminary studies were conducted to determine the suitable dose of each of the mutagens. These preliminary studies revealed that the suitable dose for EMS was 0.25% and 0.025% for MMS (volume by volume). The mutagenic solu tions were prepared in phosphate buffer adjusted to a pH of 7.0. Only one con centration of GA of 1000 ppm was used as this was found to be the most suitable concentration (Narsinghani and Kumar 1971). The following treatments were used:-1. EMS 0.25% 4. MMS 0.025%+GA (1000 ppm) 2. EMS 0.25%+GA (1000 ppm) 5. GA (Control) 1000ppm 3. MMS 0.025% 6. Control-distilled water. The seeds were first presoaked for two hours in distilled water and then im mersed in solutions of mutagens for ten hours. At the end of the treatment the seeds were washed with distilled water and sown in the field. Plants in the M1 generation were tagged individually and chromosomal aberrations recorded. The

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Mutation research in peas

Cited by 5 publications

References 0 publications

Impact of ethyl methane sulphonate on M1 attributes of Groundnut with special emphasis of amino acid profiling

Impact of ethyl methane sulphonate on M1 attributes of Groundnut with special emphasis of amino acid profiling

Mutations in peas induced by diethylsulphate and X-rays

Gibberellic Acid Protection against EMS and MMS Induced Damage to Chromosomes in <i>Pisum sativum</i> L

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