Mutation of the miaA gene of Agrobacterium tumefaciens results in reduced vir gene expression

Gray, J A; Wang, Juan; Gelvin, Stanton B.

doi:10.1128/jb.174.4.1086-1098.1992

Cited by 78 publications

(63 citation statements)

References 68 publications

(64 reference statements)

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“…The miaA product is a tRNA N 6 -isopentenyladenosine synthetase involved in the first step of the transformation of the adenosine located at position 37 (ms 2 i 6 A37) of some tRNAs into 2-methyl-N 6 -isopentenyladenosine. Recently, this tRNA modification was found to be essential for optimal expression of virulence factors in the bacterial pathogens Agrobacterium tumefaciens and Shigella flexneri (Durand et al, 1997;Gray et al, 1992). In the latter organism, it has been reported that a miaA mutant displayed a tenfold reduction in transcription of the virF regulon due to a post-transcriptional downregulation of VirF activator expression (Durand et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence

et al. 2004

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The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence The Yersinia pseudotuberculosis chromosome contains a seven-gene polycistronic unit (the pmrF operon) whose products share extensive homologies with their pmrF counterparts in Salmonella enterica serovar Typhimurium (S. typhimurium), another Gram-negative bacterial enteropathogen. This gene cluster is essential for addition of 4-aminoarabinose to the lipid moiety of LPS, as demonstrated by MALDI-TOF mass spectrometry of lipid A from both wild-type and pmrF-mutated strains. As in S. typhimurium, 4-aminoarabinose substitution of lipid A contributes to in vitro resistance of Y. pseudotuberculosis to the antimicrobial peptide polymyxin B. Whereas pmrF expression in S. typhimurium is mediated by both the PhoP-PhoQ and PmrA-PmrB two-component regulatory systems, it appears to be PmrA-PmrB-independent in Y. pseudotuberculosis, with the response regulator PhoP interacting directly with the pmrF operon promoter region. This result reveals that the ubiquitous PmrA-PmrB regulatory system controls different regulons in distinct bacterial species. In addition, pmrF inactivation in Y. pseudotuberculosis has no effect on bacterial virulence in the mouse, again in contrast to the situation in S. typhimurium. The marked differences in pmrF operon regulation in these two phylogenetically close bacterial species may be related to their dissimilar lifestyles.

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Section: Discussionmentioning

confidence: 99%

The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence

et al. 2004

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“…The presence of modified nucleosides in tRNA was also shown to be important for virulence of Shigella (Durant & Davis, 1997), Agrobacterium tumefaciens (Gray et al, 1992), Pseudomonas aeruginosa (Sage et al, 1997;Urbonavicius et al, 2002) and Pseudomonas syringae (Kinscherf & Willis, 2002). Isolation of mutants with defective tRNA modification activity has frequently been associated with altered pathogenicity or metabolic activity phenotypes.…”

Section: Discussionmentioning

confidence: 99%

The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes

Ahn

Jia

et al. 2004

Microbiology

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“…These proteins include Agrobacterium tumefaciens vir and Shigella flexneri VirF (Gray et al 1992;Durand et al 1997Durand et al , 2000. In addition, Streptomyces coelicolor bld mutant phenotype is suppressed by overexpression of tRNA CAA Leu , which also likely contains the i 6 A37 modification (Pettersson and Kirsebom 2011).…”

Section: Implications For Comparative Functional Genomics Of Trna Isomentioning

confidence: 99%

The i⁶A37 tRNA modification is essential for proper decoding of UUX-Leucine codons during rpoS and iraP translation

Aubee

Olu

Thompson

2016

RNA

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The translation of rpoS (σ S ), the general stress/stationary phase sigma factor, is tightly regulated at the post-transcriptional level by several factors via mechanisms that are not clearly defined. One of these factors is MiaA, the enzyme necessary for the first step in the N 6 -isopentyl-2-thiomethyladenosinemethyladenosine 37 (ms 2 i 6 A37) tRNA modification. We tested the hypothesis that an elevated UUX-Leucine/total leucine codon ratio can be used to identify transcripts whose translation would be sensitive to loss of the MiaA-dependent modification. We identified iraP as another candidate MiaA-sensitive gene, based on the UUX-Leucine/ total leucine codon ratio. An iraP-lacZ fusion was significantly decreased in the absence of MiaA, consistent with our predictive model. To determine the role of MiaA in UUX-Leucine decoding in rpoS and iraP, we measured β-galactosidasespecific activity of miaA − rpoS and iraP translational fusions upon overexpression of leucine tRNAs. We observed suppression of the MiaA effect on rpoS, and not iraP, via overexpression of tRNA LeuX but not tRNA LeuZ . We also tested the hypothesis that the MiaA requirement for rpoS and iraP translation is due to decoding of UUX-Leucine codons within the rpoS and iraP transcripts, respectively. We observed a partial suppression of the MiaA requirement for rpoS and iraP translational fusions containing one or both UUX-Leucine codons removed. Taken together, this suggests that MiaA is necessary for rpoS and iraP translation through proper decoding of UUX-Leucine codons and that rpoS and iraP mRNAs are both modification tunable transcripts (MoTTs) via the presence of the modification.

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Mutation of the miaA gene of Agrobacterium tumefaciens results in reduced vir gene expression

Abstract: vir regulon expression in Agrobacterium tumefaciens involves both chromosome-and Ti-plasmid-encoded gene products. We (7,31,33,58

Cited by 78 publications

References 68 publications

The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence

The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence

The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes

The i⁶A37 tRNA modification is essential for proper decoding of UUX-Leucine codons during rpoS and iraP translation

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Mutation of the miaA gene of Agrobacterium tumefaciens results in reduced vir gene expression

Abstract: vir regulon expression in Agrobacterium tumefaciens involves both chromosome-and Ti-plasmid-encoded gene products. We (7,31,33,58

Cited by 78 publications

References 68 publications

The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence

The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence

The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes

The i6A37 tRNA modification is essential for proper decoding of UUX-Leucine codons during rpoS and iraP translation

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The i⁶A37 tRNA modification is essential for proper decoding of UUX-Leucine codons during rpoS and iraP translation