Mutation of the ARA7/AtRabF2b Gene, That Increases the Content of the Ara7 Protein Regulating Endocytic Trafficking Pathways, Improves Salt Tolerance of the Arabidopsis thaliana (L.) Heynh Plants

Сергиенко, О. В.; Халилова, Л. А.; Orlova, Yu. V.; Shuvalov, Alexey; Myasoedov, N. A.; Karpichev, Igor V.

doi:10.1134/s1021443722010198

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…Research also confirms the phenomenon of vacuolization and the fusion of provacuoles into large vacuoles located in the center of the cell under the effect of salt stress [60][61][62]. Moreover, research has reported a correlation between the effect of NaCl stress and the appearance of vacuolization and PCD as an effect of ionic imbalances present in salt treatments in Arabidopsis [63]. Cell vacuolization occurs not only under salt stress, but also under heavy-metal stress [64][65][66].…”

Section: Discussionsupporting

confidence: 62%

Cytological Applications of the Vacuolization Phenomenon as a Means of Determining Saline Cytotoxicity

Emilian,

Ioan,

Irina

et al. 2023

Applied Sciences

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Salt stress induces cytotoxicity at the cellular level, influencing the vacuolization process, disrupting mitotic division, and thus inhibiting plant growth. The results for a range of species used in agriculture have shown that high soil salt levels affect germination, chlorophyl content and yield. In this study, an experiment was carried out in the laboratory using NaCl concentration treatments of 0, 100, 125 and 150 mM on sunflower seeds of the inbred line HA-89 obtained from the USDA gene bank. For the experiment, the seeds were germinated in a salt solution and analyzed cytologically by calculating the mitotic index, chromosomal aberration index, provacuolar index and vacuolization index. Following our cytological studies, we observed that the vacuolization phenomenon was caused by salt stress and progressively accentuated by the salt concentration levels and exposure times. The formation of vacuolized cells is due to the fusion of provacuoles, which contributes to a uniform or non-uniform distribution of genetic material around them. According to our results, the vacuolization index showed high values depending on the NaCl concentration and stress exposure time. Similarly, high salt concentrations significantly decreased the mitotic index and increased the chromosomal aberration index. The effect of salt stress causes cell vacuolization, a decrease in the mitotic index and an increase in the number of chromosomal aberrations in meristematic tissues, inhibiting growth and development and consequently leading to a reduction in productivity per unit area.

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Section: Discussionsupporting

confidence: 62%

Cytological Applications of the Vacuolization Phenomenon as a Means of Determining Saline Cytotoxicity

Emilian,

Ioan,

Irina

et al. 2023

Applied Sciences

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“…There is evidence that members of the RabF subfamily play a role in salt stress tolerance, such as the mutation that increases AtRab ARA7 (AtRabF2b) protein levels, which resulted in increased long-term tolerance to salt stress when compared to WT plants. In addition, an increased mass of roots and leaves, increased K + content, and decreased Na + content have been reported under these conditions [14]. Moreover, AtRabF1 of A. thaliana transports endosomal vesicles and plays a role in stress response.…”

Section: Differential Expression Of Rab Gtpases Of the Endocytic And ...mentioning

confidence: 91%

“…Transport to and from specific compartments for recycling and elimination of macromolecules occurs through an intracellular vesicular trafficking system. While vesicular trafficking has conventionally been viewed as a fundamental process in both animals and plants; primarily involved in the transport and renewal of cellular structures, there is mounting evidence suggesting its pivotal role in stress adaptation [13][14][15][16][17][18]. For instance, research has demonstrated that a mutation in RabF2b of the endocytic pathway enhances salt stress tolerance [14], while RabA1 from the TGN and PM pathway is essential for salinity tolerance in Arabidopsis thaliana [15].…”

Section: Introductionmentioning

confidence: 99%

Identification, Classification, and Transcriptional Analysis of Rab GTPase Genes from Tomato (Solanum lycopersicum) Reveals Salt Stress Response Genes

Soto,

San Martín-Davison,

Salinas-Cornejo

et al. 2024

Genes

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Salinity in plants generates an osmotic and ionic imbalance inside cells that compromises the viability of the plant. Rab GTPases, the largest family within the small GTPase superfamily, play pivotal roles as regulators of vesicular trafficking in plants, including the economically important and globally cultivated tomato (Solanum lycopersicum). Despite their significance, the specific involvement of these small GTPases in tomato vesicular trafficking and their role under saline stress remains poorly understood. In this work, we identified and classified 54 genes encoding Rab GTPases in cultivated tomato, elucidating their genomic distribution and structural characteristics. We conducted an analysis of duplication events within the S. lycopersicum genome, as well as an examination of gene structure and conserved motifs. In addition, we investigated the transcriptional profiles for these Rab GTPases in various tissues of cultivated and wild tomato species using microarray-based analysis. The results showed predominantly low expression in most of the genes in both leaves and vegetative meristem, contrasting with notably high expression levels observed in seedling roots. Also, a greater increase in gene expression in shoots from salt-tolerant wild tomato species was observed under normal conditions when comparing Solanum habrochaites, Solanum pennellii, and Solanum pimpinellifolium with S. lycopersicum. Furthermore, an expression analysis of Rab GTPases from Solanum chilense in leaves and roots under salt stress treatment were also carried out for their characterization. These findings revealed that specific Rab GTPases from the endocytic pathway and the trans-Golgi network (TGN) showed higher induction in plants exposed to saline stress conditions. Likewise, disparities in gene expression were observed both among members of the same Rab GTPase subfamily and between different subfamilies. Overall, this work emphasizes the high degree of conservation of Rab GTPases, their high functional diversification in higher plants, and the essential role in mediating salt stress tolerance and suggests their potential for further exploration of vesicular trafficking mechanisms in response to abiotic stress conditions.

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“…Genomic DNA from A. thaliana leaves was isolated using a CTAB method described previously [ 47 ] with some modifications, as follows. A total of 200 mg of plant tissue in a 1.5 mL test tube was ground with Celite and 200 µL of MicroPrep lysis buffer (0.088 M Sorbitol, 0.075 M Tris, 0.014 M EDTA, 0.5 M NaCl, 0.5% CTAB, and 0.5% Sarkosyl, pH 7.5) containing 0.1% sodium bisulfite (added just before use); then, 550 μL more buffer was added to the slurry.…”

Section: Methodsmentioning

confidence: 99%

Involvement of the Membrane Nanodomain Protein, AtFlot1, in Vesicular Transport of Plasma Membrane H+-ATPase in Arabidopsis thaliana under Salt Stress

Халилова

Lobreva

Nedelyaeva

et al. 2023

IJMS

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The aim of this study was to elucidate whether the membrane nanodomain protein AtFlot1 is involved in vesicular transport pathways and regulation of the P-type H+-ATPase content in plasma membrane of A. thaliana under salt stress. Transmission electron microscopy revealed changes in the endosomal system of A. thaliana root cells due to knockout mutation SALK_205125C (Atflot1ko). Immunoblotting of the plasma membrane-enriched fractions isolated from plant organs with an antibody to the H+-ATPase demonstrated changes in the H+-ATPase content in plasma membrane in response to the Atflot1ko mutation and salt shock. Expression levels of the main H+-ATPase isoforms, PMA1 and PMA2, as well as endocytosis activity of root cells determined by endocytic probe FM4-64 uptake assay, were unchanged in the Atflot1ko mutant. We have shown that AtFlot1 participates in regulation of the H+-ATPase content in the plasma membrane. We hypothesized that AtFlot1 is involved in both exocytosis and endocytosis, and, thus, contributes to the maintenance of cell ion homeostasis under salt stress. The lack of a pronounced Atflot1ko phenotype under salt stress conditions may be due to the assumed ability of Atflot1ko to switch vesicular transport to alternative pathways. Functional redundancy of AtFlot proteins may play a role in the functioning of these alternative pathways.

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Mutation of the ARA7/AtRabF2b Gene, That Increases the Content of the Ara7 Protein Regulating Endocytic Trafficking Pathways, Improves Salt Tolerance of the Arabidopsis thaliana (L.) Heynh Plants

Cited by 5 publications

References 27 publications

Cytological Applications of the Vacuolization Phenomenon as a Means of Determining Saline Cytotoxicity

Cytological Applications of the Vacuolization Phenomenon as a Means of Determining Saline Cytotoxicity

Identification, Classification, and Transcriptional Analysis of Rab GTPase Genes from Tomato (Solanum lycopersicum) Reveals Salt Stress Response Genes

Involvement of the Membrane Nanodomain Protein, AtFlot1, in Vesicular Transport of Plasma Membrane H+-ATPase in Arabidopsis thaliana under Salt Stress

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