Mutation of Serine protease 1 Induces Male Sterility in Bombyx mori

Xu, Xia; Wang, Yaohui; Chen, Jine; Du, Xin; Yao, Lusong; Xu, Jun; Zhang, Yong; Huang, Yongping; Wang, Yongqiang

doi:10.3389/fphys.2022.828859

Cited by 4 publications

(7 citation statements)

References 52 publications

(56 reference statements)

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“…Our previous research found that mutation of Ser1 leads to male sterility in B . mori [ 28 ], however, how Ser1 is particularly involved in the reproduction process remains unknown. We subsequently identified the gene in the S .…”

Section: Resultsmentioning

confidence: 99%

“…Despite our previous work found that the mutation of Ser1 causes male sterility in B . mori [ 28 ], how its particular role in the reproductive process of any lepidopteran pest remains incompletely known. The effects on the reproductive tissues and cells induced by the mutation, as well as the biochemical reactions it may involve in are not investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies on B . mori have demonstrated that knockout of Ser2 or Serine Protease 1 ( Ser1 ) leads to male sterility [ 28 , 29 ]. While some studies have explored the reproductive effects of trypsin-type serine proteases in lepidopteran insects, their specific roles in regulating processes like spermatogenesis, sperm activation, and spermatophore formation remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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SPSL1 is essential for spermatophore formation and sperm activation in Spodoptera frugiperda

Qian,

Yang,

et al. 2023

PLoS Genet

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The reproductive process in various species has undergone evolutionary adaptations at both the physiological and molecular levels, playing a significant role in maintaining their populations. In lepidopteran insects, the spermatophore is a unique structure formed in the female reproductive system, in which sperm storage and activation take place. It is known that the formation of the spermatophore is regulated by seminal fluid proteins derived from males. However, studies investigating the genetic mechanisms behind spermatophore formation in lepidopterans have been limited. In this study, our focus was on SPSL1, a gene that encodes a trypsin-type seminal fluid protein in Spodoptera frugiperda, a pest species with global invasive tendencies. Our findings revealed that SPSL1 expression was predominantly observed in the male reproductive tracts, and the disruption of this gene resulted in male sterility. Surprisingly, fluorescence analysis indicated that the absence of SPSL1 did not affect spermatogenesis or sperm migration within the male reproductive system. However, when females mated with SPSL1-mutant males, several defects were observed. These included disruptions in spermatophore formation, sperm activation in the copulatory bursae, and sperm migration into the spermathecae. Additionally, mass spectrometry analysis highlighted reduced levels of energy-related metabolites, suggesting that SPSL1 plays an essential role in promoting hydrolysis reactions during copulation. Consequently, our study demonstrates that SPSL1 is crucial for male fertility due to its functions in spermatophore formation and sperm activation. This research provides valuable insights into the genetic factors underlying reproductive processes in lepidopteran insects and sheds light on potential strategies for controlling invasive pest populations.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SPSL1 is essential for spermatophore formation and sperm activation in Spodoptera frugiperda

Qian,

Yang,

et al. 2023

PLoS Genet

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“…RNAi of PCE3 gene reduces female oviposition and leads to eggs being unable to hatch (Zheng et al, 2021). In B. mori , mutation of BmSer1 leads to male sterility, but has no effect on female fertility (Xu, Wang, et al, 2022). Some SP/SPH genes display high or specific expressions in antennae and leg, but their physiological functions remain to be investigated, and likely to have other undiscovered biological functions in insect biology.…”

Section: Resultsmentioning

confidence: 99%

Identification and expression profiling of serine protease‐related genes in Tenebrio molitor

Xiao

Wang

et al. 2022

Arch Insect Biochem Physiol

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In insects, serine proteases and serine protease homologs (SPs/SPHs) are involved in a variety of physiological processes including digestion, development, and immunity.Here, we identified 112 SP and 88 SPH genes in the genome of the yellow mealworm, Tenebrio molitor. Based on the features of domain structure, they were divided into "S" group containing single Tryp-SPc or Tryp-SPHc domain, "C" group containing 1-4 CLIP domain (CLIPA-D) and "M"

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“…Our results suggest that the Ser2 gene regulates the male reproductive capacity and has the potential as a better target gene for pest control, especially in S. litura . In the silkworm, B. mori , the seminal fluid protein genes have been identified as the useful target genes for SIT ( Xu et al, 2020 ; Xu et al, 2022 ). Sustainability is an important factor in pest control.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-mediated Serine protease 2 disruption induces male sterility in Spodoptera litura

et al. 2022

Front. Physiol.

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Male fertility is essential for reproduction and population growth in animals. Many factors affect male fertility, such as courtship behavior, sperm quantity, and sperm motility, among others. Seminal Fluid Proteins (SFPs) are vital components of seminal fluid in the male ejaculate, which affect male fertility, sperm activation, and female ovulation. However, the knowledge of SFPs is insufficient; the function of many SFPs remains unknown, and most described functions were mainly characterized in Drosophila or other laboratory models. Here, we focus on the Serine protease 2 (Ser2) gene in the lepidopteran pest Spodoptera litura. The Ser2 gene was specifically expressed in male adults. Disruption of the Ser2 gene mediated by CRISPR/Cas9 induced male sterility but females remained fertile. PCR-based detection of the next-generation mutants showed that male sterility was stably inherited. The qRT-PCR analysis of SlSer2 mutants showed that motor protein family genes and structural protein family genes were down-regulated, while protein modification family genes were up-regulated, suggesting that SlSer2 may be involved in sperm movement and activity. These results demonstrate that Ser2 is an important component of SFPs in seminal fluid and was identified for a useful sterile gene for pest control that may lead to new control strategies for lepidopteran insect pests such as S. litura.

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Mutation of Serine protease 1 Induces Male Sterility in Bombyx mori

Cited by 4 publications

References 52 publications

SPSL1 is essential for spermatophore formation and sperm activation in Spodoptera frugiperda

SPSL1 is essential for spermatophore formation and sperm activation in Spodoptera frugiperda

Identification and expression profiling of serine protease‐related genes in Tenebrio molitor

CRISPR/Cas9-mediated Serine protease 2 disruption induces male sterility in Spodoptera litura

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