Mutation of Panax ginseng genes during long-term cultivation of ginseng cell cultures

Kiselev, Konstantin V.; Shumakova, O. A.; Tchernoded, G. K.

doi:10.1016/j.jplph.2011.01.028

Cited by 19 publications

(13 citation statements)

References 28 publications

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“…In our analysis, we found that most of the detected single nucleotide substitutions that accumulated during A. thaliana growth and aging were A:T?G:C transitions, which suggests that the transitions did not result from an ultraviolet light-induced mutagenesis. The data correlate with observations that most of the single nucleotide substitutions that accumulated during the long-term cultivation of Panax ginseng and Oryza sativa callus cultures were also A:T?G:C transitions (Noro et al 2007;Kiselev et al 2009Kiselev et al , 2011Kiselev et al , 2013. However, in contrast to the somatic mutagenesis observed for the A. thaliana plants in this study, no insertions or deletions were detected in the endogenous gene sequences analyzed for P. ginseng in cell cultures that had been propagated for more than 20 years (Kiselev et al 2009(Kiselev et al , 2011(Kiselev et al , 2013.…”

Section: Types Of Somatic Mutationssupporting

confidence: 89%

“…Ns is the total number of nucleotide substitutions in all clones obtained from a certain time point; Ni is the total number of insertions in all clones obtained from a certain time point; Nd is the total number of deletions in all clones obtained from a certain time point; L is the length of the analyzed gene fragment; P is the length of the primers (in nucleotides) used for amplification; and Nc is the total number of clones for the sequenced target gene (Kiselev et al 2009(Kiselev et al , 2011(Kiselev et al , 2013. The frequency of nucleotide substitutions per 1,000 nt was determined using the following formula:…”

Section: Cloning and Dna Sequencingmentioning

confidence: 99%

“…The authors suggest that even long-lived clonal plants are vulnerable to senescence. It has also been shown that single nucleotide substitutions accumulate in several endogenous genes and transgenes during the long-term cultivation of plant cell cultures (Noro et al 2007;Kiselev et al 2009Kiselev et al , 2011Kiselev et al , 2013. To our knowledge, there is no other experimental data where somatic mutagenesis has been investigated in relation to ageing in plants or plant cell cultures.…”

Section: Introductionmentioning

confidence: 99%

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Age-associated alterations in the somatic mutation level in Arabidopsis thaliana

et al. 2014

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Age-related accumulation of somatic mutations has been implicated as an important mechanism contributing to ageing and various diseases in animals. However, it is unclear whether the somatic mutation frequency changes as plants age and to what extent somatic mutagenesis contributes to plant ageing or senescence. The contribution of somatic mutagenesis to plant ageing and senescence was not investigated. We used the species Arabidopsis thaliana to study whether an increasing chronological age of an annual plant species influences the total amount and molecular spectrum of somatic DNA mutations. The number of small-scale somatic mutations was studied in nine randomly chosen A. thaliana control DNA regions, including those in the nuclear genome (Actin2 coding region, Actin2 3 0 -UTR region, CMT3 methyltrasferase gene, tRNAPro, and ITS1-5.8rRNA-ITS2), mitochondrial DNA (cytochrome oxidase C, a gene encoding an unnamed protein, and a noncoding mitochondrial DNA region), and chloroplast DNA (rbcL gene). We found that the frequency of single nucleotide substitutions, insertions, and deletions increased considerably during the A. thaliana growth and development from the seeds to the 12-week-old plants. The vast majority of nucleotide substitutions (*86 %) were transitions with A:T?G:C transitions being the most frequent type of substitution. The data indicate that at least some plant DNA regions accumulate point mutations during plant aging.

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Section: Types Of Somatic Mutationssupporting

confidence: 89%

Section: Cloning and Dna Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Age-associated alterations in the somatic mutation level in Arabidopsis thaliana

et al. 2014

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“…The DNA was extracted with the modified Echt's method (echt et al 1992;Kiselev et al 2011) from the specimens collected in the Maagan Michael Quarry (Israel) and oxbow lake of the Yeya River (Russia), where the sizes of specimens were allowed to detach from several apical parts of thalli without obvious deterioration. A partial rbcL gene was amplified with primers RH1 and1385R (manhaRt et al 1994), and PCR products were directly sequenced using the ABI Big Dye Terminator v.3.1 Cycle Sequencing kit according to the manufacturer's instructions and the ABI 3130 genetic analyzer at the Institute of Biology and Soil Science FEB RAS.…”

Section: Methodsmentioning

confidence: 99%

Chara globata Mig. (Streptophyta: Charales): rare species revised

Romanov¹,

Gontcharov²,

Barınova³

2015

Fottea

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Abstract:A rare species Chara globata miG. has a distinct habit and may be referred to as a flagship species. Here we describe a wide range of vegetative and reproductive morphologies in C. globata in localities distant from the locus classicus. In contrast to the known holotype features, the specimens studied consistently displayed a generally tylacanthous diplostichous stem cortex which is in agreement with protologue. Based on morphology, phylogenetic affinity (this study) and results of breeding experiments (PRoctoR 1971(PRoctoR , 1975, we proposed that C. globata should be transferred to the section Chara. Current data suggest that C. globata is a species with a disjunctive distribution, mainly in the arid and semiarid regions of Eurasia. Specimens collected from the end of the 1960s to the beginning of the 1970s confirmed the existence C. globata in Western Asia. The species was also recorded in Europe and the Sahara-Arabian Desert for the first time.

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“…Many scientists studied the Panax ginseng via the bioinformatics perspective [17,18], such as Yu-Jin Kim, et al explored that the related genes of Panax ginseng and their expression against environmental stresses [19]. Konstantin V. Kiselev, et al, tended to think that mutation of Panax ginseng genes may be happened during long-term cultivation [20]. Wan-jing Liu, et al, studied the HMGS and HMGR Genes from Panax notoginseng via bioinformatics tools [21].…”

Section: Introductionmentioning

confidence: 99%

Codon Usage Study on Chloroplast Genome in Medicinal Plant <i>Panax Ginseng</i>

Li¹,

Zhang²,

Jingqi³

et al. 2017

IJHNM

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Abstract:Panax ginseng is usually used as a famous plant medicine in many countries. In order to study the codon usage in whole chloroplast genome in Panax Ginseng, whole chloroplast genome sequence is analyzed via codonW software and some results such as relationships between the codon adaptation index, frequency of optimal codons, codon bias index, effective number of codons, especially the relative synonymous codon usage are all discussed. Furthermore, PR2-bias plot, histogram GC content, Codon Bias Index are also analyzed for exploring the sequence characteristics of chloroplast genome in Panax Ginseng. These results showed that codon usage bias in the chloroplast genome from Panax Ginseng is weak, and the relative results could lay a foundation for other further study on the medicinal plant Panax Ginseng.

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Mutation of Panax ginseng genes during long-term cultivation of ginseng cell cultures

Cited by 19 publications

References 28 publications

Age-associated alterations in the somatic mutation level in Arabidopsis thaliana

Age-associated alterations in the somatic mutation level in Arabidopsis thaliana

Chara globata Mig. (Streptophyta: Charales): rare species revised

Codon Usage Study on Chloroplast Genome in Medicinal Plant <i>Panax Ginseng</i>

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Mutation of Panax ginseng genes during long-term cultivation of ginseng cell cultures

Cited by 19 publications

References 28 publications

Age-associated alterations in the somatic mutation level in Arabidopsis thaliana

Age-associated alterations in the somatic mutation level in Arabidopsis thaliana

Chara globata Mig. (Streptophyta: Charales): rare species revised

Codon Usage Study on Chloroplast Genome in Medicinal Plant &lt;i&gt;Panax Ginseng&lt;/i&gt;

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Codon Usage Study on Chloroplast Genome in Medicinal Plant <i>Panax Ginseng</i>