Mutation of N-linked glycosylation in EpCAM affected cell adhesion in breast cancer cells

Li, Xue; Gao, Jiujiao; Sun, Yunyan; Zhang, Dandan; Liu, Tingjiao; Qiu, Yan; Yang, Xuesong

doi:10.1515/hsz-2016-0232

Cited by 19 publications

(16 citation statements)

References 29 publications

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“…Addition of 5-fluorouracil to the deglycosylated mutant cell lines enhanced the cell cytotoxicity, promoting apoptosis by downregulating the expression of the anti-apoptotic marker Bcl-2 and upregulating the expression of the pro-apoptotic proteins Bax and Caspase 3 [42]. EpCAM expression was associated with cell adhesion, and N-glycosylation mutation of EpCAM decreased adhesion capacity in breast cancer cells [43]. Therefore, it is of high interest to study glycosylation of EpCAM or perhaps other types of post-translational modifications that contribute to the onset of cancer.…”

Section: N-linked Glycosylationmentioning

confidence: 97%

Revisiting the Roles of Pro-Metastatic EpCAM in Cancer

et al. 2020

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Epithelial cell adhesion molecule (EpCAM) is a cell surface protein that was discovered as a tumour marker of epithelial origins nearly four decades ago. EpCAM is expressed at basal levels in the basolateral membrane of normal epithelial cells. However, EpCAM expression is upregulated in solid epithelial cancers and stem cells. EpCAM can also be found in disseminated tumour cells and circulating tumour cells. Various OMICs studies have demonstrated that EpCAM plays roles in several key biological processes such as cell adhesion, migration, proliferation and differentiation. Additionally, EpCAM can be detected in the bodily fluid of cancer patients suggesting that EpCAM is a pathophysiologically relevant anti-tumour target as well as being utilized as a diagnostic/prognostic agent for a variety of cancers. This review will focus on the structure-features of EpCAM protein and discuss recent evidence on the pathological and physiological roles of EpCAM in modulating cell adhesion and signalling pathways in cancers as well as deliberating the clinical implication of EpCAM as a therapeutic target.

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Section: N-linked Glycosylationmentioning

confidence: 97%

Revisiting the Roles of Pro-Metastatic EpCAM in Cancer

et al. 2020

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“…Notably, women with Lynch syndrome have a high risk of ovarian cancer. Liu et al reported that elevated EPCAM expression is correlated with metastasis and cell adhesion in breast cancer tissue 80. In addition to being observed in epithelial malignancies, EPCAM overexpression is associated with larger tumor size, lymph node metastasis and a poorer prognosis in gastric cancer 81.…”

Section: Discussionmentioning

confidence: 99%

<p>Integrated Analysis To Identify Molecular Biomarkers Of High-Grade Serous Ovarian Cancer</p>

Si¹,

Zhang²,

Cao³

et al. 2019

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PurposeOvarian cancer is the leading cause of gynecologic cancer-related death worldwide. Early diagnosis of ovarian cancer can significantly improve patient prognosis. Hence, there is an urgent need to identify key diagnostic and prognostic biomarkers specific for ovarian cancer. Because high-grade serous ovarian cancer (HGSOC) is the most common type of ovarian cancer and accounts for the majority of deaths, we identified potential biomarkers for the early diagnosis and prognosis of HGSOC.MethodsSix datasets (GSE14001, GSE18520, GSE26712, GSE27651, GSE40595, and GSE54388) were downloaded from the Gene Expression Omnibus database for analysis. Differentially expressed genes (DEGs) between HGSOC and normal ovarian surface epithelium samples were screened via integrated analysis. Hub genes were identified by analyzing protein–protein interaction (PPI) network data. The online Kaplan-Meier plotter was utilized to evaluate the prognostic roles of these hub genes. The expression of these hub genes was confirmed with Oncomine datasets and validated by quantitative real-time PCR and Western blotting.ResultsA total of 103 DEGs in patients with HGSOC—28 upregulated genes and 75 downregulated genes—were successfully screened. Enrichment analyses revealed that the upregulated genes were enriched in cell division and cell proliferation and that the downregulated genes mainly participated in the Wnt signaling pathway and various metabolic processes. Ten hub genes were associated with HGSOC pathogenesis. Seven overexpressed hub genes were partitioned into module 1 of the PPI network, which was enriched in the cell cycle and DNA replication pathways. Survival analysis revealed that MELK, CEP55 and KDR expression levels were significantly correlated with the overall survival of HGSOC patients (P < 0.05). The RNA and protein expression levels of these hub genes were validated experimentally.ConclusionBased on an integrated analysis, we propose the further investigation of MELK, CEP55 and KDR as promising diagnostic and prognostic biomarkers of HGSOC.

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“…The between hub gene and drug designed in database of drug bank. It is helpful in decreasing or increasing expression of protein expression of the hub genes [18].…”

Section: Functional Enrichment Softwarementioning

confidence: 99%