Mutation of KCNK5 or Kir3.2 Potassium Channels in Mice Does Not Change Minimum Alveolar Anesthetic Concentration

Gerstin, Karin M.; Gong, Diane; Abdallah, Mona; Winegar, Bruce D.; Eger, Edmond I.; Gray, Andrew T.

doi:10.1213/01.ane.0000056921.15974.ec

Cited by 34 publications

(34 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Information on the likely relevance of two-pore-domain channels to general anesthesia may come from genetically modified animals in which certain members of the superfamily are either modified or excluded. A recent study (Gerstin et al, 2003) on such a knock-out animal has used this approach and concluded that TASK-2 (KCNK5) channels are unlikely to be important for general anesthesia. Because the anatomical distribution and anesthetic sensitivities of two-pore-domain channels vary so greatly, the lack of importance of one member of the family cannot easily be extrapolated to other members, and the role of the channels we have studied (TREK-1 and TASK-3) in general anesthesia remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Two-Pore-Domain K⁺ Channels Are a Novel Target for the Anesthetic Gases Xenon, Nitrous Oxide, and Cyclopropane

Gruß

Bushell²,

Bright³

et al. 2004

Mol Pharmacol

285

192

View full text Add to dashboard Cite

Nitrous oxide, xenon, and cyclopropane are anesthetic gases that have a distinct pharmacological profile. Whereas the molecular basis for their anesthetic actions remains unclear, they behave very differently to most other general anesthetics in that they have little or no effect on GABA A receptors, yet strongly inhibit the N-methyl-D-aspartate subtype of glutamate receptors. Here we show that certain members of the two-poredomain

show abstract

Section: Discussionmentioning

confidence: 99%

Two-Pore-Domain K⁺ Channels Are a Novel Target for the Anesthetic Gases Xenon, Nitrous Oxide, and Cyclopropane

Gruß

Bushell²,

Bright³

et al. 2004

Mol Pharmacol

285

192

View full text Add to dashboard Cite

show abstract

“…Despite activation of TASK-2 by volatile anesthetics there was little evidence supporting a role for TASK-2 in mediating anesthesia. TASK-2 expression in the spinal cord suggested that its activation may significantly affect motor and sensory pathways during anesthesia however sensitivity to volatile anesthetics was not affected in TASK-2 knockout mice [57]. TASK-2 channels were also inhibited by local anesthetics and, like many other K2P channels, were most sensitive to bupivacaine [107] whereas TALK-2 was relatively insensitive [41].…”

Section: Talk Pharmacological Characteristicsmentioning

confidence: 99%

Biophysical, pharmacological, and functional characteristics of cloned and native mammalian two-pore domain K+ channels

Lotshaw

2007

Cell Biochem Biophys

163

185

View full text Add to dashboard Cite

The mammalian family of two-pore domain K+ (K2P) channel proteins are encoded by 15 KCNK genes and subdivided into six subfamilies on the basis of sequence similarities: TWIK, TREK, TASK, TALK, THIK, and TRESK. K2P channels are expressed in cells throughout the body and have been implicated in diverse cellular functions including maintenance of the resting potential and regulation of excitability, sensory transduction, ion transport, and cell volume regulation, as well as metabolic regulation and apoptosis. In recent years K2P channel isoforms have been identified as important targets of several widely employed drugs, including: general anesthetics, local anesthetics, neuroprotectants, and anti-depressants. An important goal of future studies will be to identify the basis of drug actions and channel isoform selectivity. This goal will be facilitated by characterization of native K2P channel isoforms, their pharmacological properties and tissue-specific expression patterns. To this end the present review examines the biophysical, pharmacological, and functional characteristics of cloned mammalian K2P channels and compares this information with the limited data available for native K2P channels in order to determine criteria which may be useful in identifying ionic currents mediated by native channel isoforms and investigating their pharmacological and functional characteristics.

show abstract

“…Effects of different inhaled anesthetics on ion channel (e.g., K ? ) [12,13] as well as in different receptors [14] have also been reported. In these biophysical studies, the concentrations of halothane, isoflurane, and sevoflurane were determined by gas chromatography [10].…”

Section: Anesthetic and Protein Interactionmentioning

confidence: 82%

Clinically Relevant Concentration Determination of Inhaled Anesthetics (Halothane, Isoflurane, Sevoflurane, and Desflurane) by 19F NMR

Mandal

Pettegrew

2008

Cell Biochem Biophys

View full text Add to dashboard Cite

Biophysical studies of protein-anesthetic interactions using nuclear magnetic resonance (NMR) spectroscopy are often conducted by the addition of micro amounts of neat inhaled anesthetic which yields much higher than clinically relevant (0.2-0.5 mM) anesthetic concentrations. We report a 19F NMR technique to measure clinically relevant inhaled anesthetic concentrations from saturated aqueous solutions of these anesthetics (halothane, isoflurane, sevoflurane, and desflurane). We use a setup with a 3-mm NMR tube (containing trifluoroacetic acid as standard), coaxially inserted in a 5-mm NMR tube containing anesthetic solution under investigation. All experiments are conducted in a 5-mm NMR probe. We also have provided standard curves for four inhaled anesthetics using NMR technique. The standard curve for each of these anesthetics is helpful in determining the prerequisite amount of aqueous anesthetic solution required to prepare clinically relevant concentrations for protein-anesthetic interaction studies.

show abstract

Mutation of KCNK5 or Kir3.2 Potassium Channels in Mice Does Not Change Minimum Alveolar Anesthetic Concentration

Cited by 34 publications

References 20 publications

Two-Pore-Domain K⁺ Channels Are a Novel Target for the Anesthetic Gases Xenon, Nitrous Oxide, and Cyclopropane

Two-Pore-Domain K⁺ Channels Are a Novel Target for the Anesthetic Gases Xenon, Nitrous Oxide, and Cyclopropane

Biophysical, pharmacological, and functional characteristics of cloned and native mammalian two-pore domain K+ channels

Clinically Relevant Concentration Determination of Inhaled Anesthetics (Halothane, Isoflurane, Sevoflurane, and Desflurane) by 19F NMR

Contact Info

Product

Resources

About

Mutation of KCNK5 or Kir3.2 Potassium Channels in Mice Does Not Change Minimum Alveolar Anesthetic Concentration

Cited by 34 publications

References 20 publications

Two-Pore-Domain K+ Channels Are a Novel Target for the Anesthetic Gases Xenon, Nitrous Oxide, and Cyclopropane

Two-Pore-Domain K+ Channels Are a Novel Target for the Anesthetic Gases Xenon, Nitrous Oxide, and Cyclopropane

Biophysical, pharmacological, and functional characteristics of cloned and native mammalian two-pore domain K+ channels

Clinically Relevant Concentration Determination of Inhaled Anesthetics (Halothane, Isoflurane, Sevoflurane, and Desflurane) by 19F NMR

Contact Info

Product

Resources

About

Two-Pore-Domain K⁺ Channels Are a Novel Target for the Anesthetic Gases Xenon, Nitrous Oxide, and Cyclopropane

Two-Pore-Domain K⁺ Channels Are a Novel Target for the Anesthetic Gases Xenon, Nitrous Oxide, and Cyclopropane