Mutation of Gly-444 inactivates the<i>S. pombe</i>malic enzyme

Viljoen, Marinda; Merwe, Marizeth van der; Subden, R. E.; Vuuren, H. J. J. Van

doi:10.1111/j.1574-6968.1998.tb13222.x

Cited by 8 publications

(1 citation statement)

References 20 publications

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“…As well as, present 1,8-cineol, linalool and pinocarveol. The 1,8-cineol and linalool are well-known substance with pronounced antimicrobial properties (34). The flower buds essential oils of E. caryophllata permitted antibacterial activity against S.aureus with inhibition zone diameter of 20 mm while E.coli inhibition zone diameter of 18 mm in oil´s concentration 50%, but concentration 25% the inhibition zone is 16 mm and 15 mm against S.aureus and E.coli respectively (Table 2).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Antibacterial Activity of Essential oils Isolated from Eucalyptus globulus Labill and Eugnia caryophyllata Thunbery (Family Myrtaceae): A comparative study

Abd al-Raouf Ammar Semysim

2018

Kufa Jour. Bio.

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The essential oils of Eucalyptus globulus Labill and Eugnia caryophyllata Thunbery analyzed by Thin Layer Chromatography TLC. The results showed that TLC plates separated five compounds from E. globulus oils and four compounds from E. caryophyllata oils with different Rf values. The obtained essential oils from leaves E. globulus and flower buds E. caryophyllata were tested on Escherichia coli and Staphylococcus aureus, by using agar well diffusion method, to determine its antibacterial activities. The strongest effected was noted in E. globulus oils against S. aureus and E.coli with diameter of zone of inhibition (25 mm and 23 mm respectively). While the E. caryophllata oils showed the least effect on S. aureus and E. coli in compare with E. globulus oils with diameter of zone of inhibition (20 mm and 18 mm respectively). We concluded that the essential oils obtained from the leaves of E. globulus offer significant antibacterial activity toward E.coli and S. aureus.

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Section: Antibacterial Activitymentioning

confidence: 99%

Antibacterial Activity of Essential oils Isolated from Eucalyptus globulus Labill and Eugnia caryophyllata Thunbery (Family Myrtaceae): A comparative study

Abd al-Raouf Ammar Semysim

2018

Kufa Jour. Bio.

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Malo-ethanolic fermentation in Saccharomyces and Schizosaccharomyces

2003

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Yeast species are divided into the K(+) or K(-) groups, based on their ability or inability to metabolise tricarboxylic acid (TCA) cycle intermediates as sole carbon or energy source. The K(-) group of yeasts includes strains of Saccharomyces, Schizosaccharomyces pombe and Zygosaccharomyces bailii, which is capable of utilising TCA cycle intermediates only in the presence of glucose or other assimilable carbon sources. Although grouped together, these yeasts have significant differences in their abilities to degrade malic acid. Typically, strains of Saccharomyces are regarded as inefficient metabolisers of extracellular malic acid, whereas strains of Sch. pombe and Z. bailii can effectively degrade high concentrations of malic acid. The ability of a yeast strain to degrade extracellular malic acid is dependent on both the efficient transport of the dicarboxylic acid and the efficacy of the intracellular malic enzyme. The malic enzyme converts malic acid into pyruvic acid, which is further metabolised to ethanol and carbon dioxide under fermentative conditions via the so-called malo-ethanolic (ME) pathway. This review focuses on the enzymes involved in the ME pathway in Sch. pombe and Saccharomyces species, with specific emphasis on the malate transporter and the intracellular malic enzyme.

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Cloning, characterisation, and heterologous expression of the Candida utilis malic enzyme gene

2006

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The Candida utilis malic enzyme gene, CME1, was isolated from a cDNA library and characterised on a molecular and biochemical level. Sequence analysis revealed an open reading frame of 1,926 bp, encoding a 641 amino acid polypeptide with a predicted molecular weight of approximately 70.2 kDa. The inferred amino acid sequence suggested a cytosolic localisation for the malic enzyme, as well as 37 and 68% homologies with the malic enzymes of Schizosaccharomyces pombe and Saccharomyces cerevisiae, respectively. Expression of the CME1 gene was subject to carbon catabolite repression and substrate induction, similar to the regulatory mechanisms observed for the C. utilis dicarboxylic acid permease. The CME1 gene was successfully expressed in S. cerevisiae under control of the S. cerevisiae PGK1 promoter and terminator. When coexpressed with the S. pombe malate permease gene (mae1), it resulted in a recombinant S. cerevisiae strain able to completely degrade 90% of the extracellular L-malate within 24 h.

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Mutation of Gly-444 inactivates theS. pombemalic enzyme

Cited by 8 publications

References 20 publications

Antibacterial Activity of Essential oils Isolated from Eucalyptus globulus Labill and Eugnia caryophyllata Thunbery (Family Myrtaceae): A comparative study

Antibacterial Activity of Essential oils Isolated from Eucalyptus globulus Labill and Eugnia caryophyllata Thunbery (Family Myrtaceae): A comparative study

Malo-ethanolic fermentation in Saccharomyces and Schizosaccharomyces

Cloning, characterisation, and heterologous expression of the Candida utilis malic enzyme gene

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