Mutation of CsARC6 affects fruit color and increases fruit nutrition in cucumber

Sun, Weifeng; Li, Xu; Huang, Hongyu; Wei, Jingwei; Zeng, Fang; Huang, Yichao; Sun, Qingqing; Miao, Weili; Tian, Yongqiang; Li, Yuhe; Gao, Lihong; Li, Xin; Gao, Hongbo

doi:10.1007/s00122-023-04337-7

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…The Cucurbitaceae fruits are well known due to their diverse size, shape, and color, and there are a number of studies that investigate their genetic basis and molecular regulation [8]. In cucumber, the CsARC6 mutation led to white fruit color, larger fruit epidermal cells, lower carotenoid content, and smaller fruits [9]. Under low-light stress, genes related to secondary metabolites, carbohydrate and amino acid metabolism, and transcriptional regulation affected the fruit expansion of watermelon [10].…”

Section: Introductionmentioning

confidence: 99%

Multi-Omics Analysis Reveals the Distinct Features of Metabolism Pathways Supporting the Fruit Size and Color Variation of Giant Pumpkin

Xia,

Chen,

Jin

et al. 2024

IJMS

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Pumpkin (Cucurbita maxima) is an important vegetable crop of the Cucurbitaceae plant family. The fruits of pumpkin are often used as directly edible food or raw material for a number of processed foods. In nature, mature pumpkin fruits differ in size, shape, and color. The Atlantic Giant (AG) cultivar has the world’s largest fruits and is described as the giant pumpkin. AG is well-known for its large and bright-colored fruits with high ornamental and economic value. At present, there are insufficient studies that have focused on the formation factors of the AG cultivar. To address these knowledge gaps, we performed comparative transcriptome, proteome, and metabolome analysis of fruits from the AG cultivar and a pumpkin with relatively small fruit (Hubbard). The results indicate that up-regulation of gene-encoded expansins contributed to fruit cell expansion, and the increased presence of photoassimilates (stachyose and D-glucose) and jasmonic acid (JA) accumulation worked together in terms of the formation of large fruit in the AG cultivar. Notably, perhaps due to the rapid transport of photoassimilates, abundant stachyose that was not converted into glucose in time was detected in giant pumpkin fruits, implying that a unique mode of assimilate unloading is in existence in the AG cultivar. The potential molecular regulatory network of photoassimilate metabolism closely related to pumpkin fruit expansion was also investigated, finding that three MYB transcription factors, namely CmaCh02G015900, CmaCh01G018100, and CmaCh06G011110, may be involved in metabolic regulation. In addition, neoxanthin (a type of carotenoid) exhibited decreased accumulation that was attributed to the down-regulation of carotenoid biosynthesis genes in AG fruits, which may lead to pigmentation differences between the two pumpkin cultivars. Our current work will provide new insights into the potential formation factors of giant pumpkins for further systematic elucidation.

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Section: Introductionmentioning

confidence: 99%

Multi-Omics Analysis Reveals the Distinct Features of Metabolism Pathways Supporting the Fruit Size and Color Variation of Giant Pumpkin

Xia,

Chen,

Jin

et al. 2024

IJMS

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“…In the meantime, Sun et al. (2023) discovered that a CsARC6 mutation affects plant development and fruit quality, in addition to causing white fruit color in cucumbers.…”

Section: Introductionmentioning

confidence: 99%

R2R3‐MYB transcription factor CsMYB60 controls mature fruit skin color by regulating flavonoid accumulation in cucumber

Xu,

Zhu,

Yuan

et al. 2024

The Plant Journal

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SUMMARYSkin color is an important trait that determines the cosmetic appearance and quality of fruits. In cucumber, the skin color ranges from white to brown in mature fruits. However, the genetic basis for this important trait remains unclear. We conducted a genome‐wide association study of natural cucumber populations, along with map‐based cloning techniques, on an F2 population resulting from a cross between Pepino (with yellow‐brown fruit skin) and Zaoer‐N (with creamy fruit skin). We identified CsMYB60 as a candidate gene responsible for skin coloration in mature cucumber fruits. In cucumber accessions with white to pale yellow skin color, a premature stop mutation (C to T) was found in the second exon region of CsMYB60, whereas light yellow cucumber accessions exhibited splicing premature termination caused by an intronic mutator‐like element insertion in CsMYB60. Transgenic CsMYB60c cucumber plants displayed a yellow‐brown skin color by promoting accumulation of flavonoids, especially hyperoside, a yellow‐colored flavonol. CsMYB60c encodes a nuclear protein that primarily acts as a transcriptional activator through its C‐terminal activation motif. RNA sequencing and DNA affinity purification sequencing assays revealed that CsMYB60c promotes skin coloration by directly binding to the YYTACCTAMYT motif in the promoter regions of flavonoid biosynthetic genes, including CsF3′H, which encodes flavonoid 3′‐hydroxylase. The findings of our study not only offer insight into the function of CsMYB60 as dominantly controlling fruit coloration, but also highlight that intronic DNA mutations can have a similar phenotypic impact as exonic mutations, which may be valuable in future cucumber breeding programs.

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“…Fruit color is considered one of the primary appearance factors that determine the purchase decision of consumers and is also an important reference for fruit classification [6][7][8][9]. Color is even a core index to evaluate the appearance quality of Korla fragrant pears, and it has an important influence on the commodity value of fresh fruits and processing products.…”

Section: Introductionmentioning

confidence: 99%

Construction of Color Prediction Model for Damaged Korla Pears during Storage Period

Zhang

Liu

et al. 2023

Applied Sciences

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In this work, to scientifically predict the color of damaged Korla fragrant pears during the storage period with lower economic loss and improved added value of the fragrant pears, eight pericarp color prediction models of damaged Korla fragrant pears during the storage period were established. These models had different membership functions, which were based on the adaptive neuro-fuzzy inference system (ANFIS). The optimal model was chosen and verified. Finally, the pericarp color of fragrant pears was accurately predicted through the degree of damage and storage time. According to the acquired test results, the pericarp brightness (L*) decreased, while both the red–green (a*) and yellow–blue (b*) values increased as the storage time prolonged. In addition, the pericarp color of the damaged fragrant pears during the storage period could be well predicted by using the ANFIS model. More specifically, the model with a membership function of trimf showed the optimal prediction effects of L*, a*, and b* (RMSE = 0.1089, R2 = 0.9773; RMSE = 0.5894, R2 = 0.9853; and RMSE = 0.2360, R2 = 0.9772). Our work provides valuable insights for the prediction of the quality of Korla fragrant pears during the storage period.

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Mutation of CsARC6 affects fruit color and increases fruit nutrition in cucumber

Cited by 5 publications

References 41 publications

Multi-Omics Analysis Reveals the Distinct Features of Metabolism Pathways Supporting the Fruit Size and Color Variation of Giant Pumpkin

Multi-Omics Analysis Reveals the Distinct Features of Metabolism Pathways Supporting the Fruit Size and Color Variation of Giant Pumpkin

R2R3‐MYB transcription factor CsMYB60 controls mature fruit skin color by regulating flavonoid accumulation in cucumber

Construction of Color Prediction Model for Damaged Korla Pears during Storage Period

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