Mutation of barley HvPDIL5-1 improves resistance to yellow mosaic virus disease without growth or yield penalties

Cheng, Chunyuan; Kan, Jinhong; Li, Shanshan; Jiang, Congcong; He, Xiaoyan; Shen, Huiquan; Xu, Ran; Li, Boqun; Feng, Zongyun; Yang, Ping

doi:10.3389/fpls.2022.1018379

Cited by 3 publications

(1 citation statement)

References 64 publications

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“…However, the above cultivars were naturally selected, and their effectiveness in the field is unstable. Genetic engineering can be used to screen resistant cultivars, improve their stability in field production, and significantly reduce the time required for traditional breeding (Li et al, 2010; Mackelprang and Lemaux, 2020; Rahman et al, 2023). However, the use genetic engineering to improve the economic and ecological traits of Pinus spp.…”

Section: Introductionmentioning

confidence: 99%

The molecular basis of pine wilt disease resistance inPinus massoniana

Wang,

Cai,

Zeng

et al. 2024

Preprint

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Pine wilt disease (PWD), caused by the pine wood nematode (PWN)Bursaphelenchus xylophilus, results in significant economic and ecological damage toPinusforests and plantations worldwide.Pinus massonianais the primary host of PWD in southern China, but its response to the PWN remains largely unstudied. Previously, we observed PWD in aP. massoniananursery that contained over 71 commonly used cultivars. Through field phenotyping, we identified two groups of PWN resistant cultivars. Resistant cultivars (RC) exhibited very low PWN carrying amounts (PCA) and had relatively low mortality, and tolerant cultivars (TC) had high PCA but low mortality. In this study, we confirmed via PWN inoculation assays that the resistant and tolerant cultivars had lower mortality rates, 10% and 11%, respectively, than other cultivars (which had a mortality rate of 83.3%). The RC had a PCA that was significantly lower than that of other cultivars, while the TC exhibited a higher PCA. To explore the molecular mechanisms underlying the response ofP. massonianato PWN, the transcriptome and metabolome of the above cultivars were profiled by high throughput sequencing. As no reference genome is available forP. massoniana, we generated a new full-length transcriptome library with iso-seq. Using the transcriptome and metabolome from differentP. massonianacultivars inoculated with PWN, we found three major PWD resistance strategies. 1) The common response strategy involved three important molecular pathways. First, synthesis of indole-3-acetic acid (IAA) and abcisic acid (ABA) were suppressed during PWN invasion, thus suppressing the synthesis of polysaccharides (especially myo-inositol and trehalose) and (-)-riboflavin through ABC transporters. Second, by inhibiting aspartic acid, the synthesis of arginine and proline through APS5 (aspartate aminotransferase 5) were suppressed. Third, by reducing cysteine, GERD (germacrene D synthase) was up-regulated, and sesquiterpenoid and triterpenoid metabolites were accumulated to resist PWN invasion. 2) The most effective resistance strategy involved the accumulation of reactive oxygen species (ROS), which enhanced jasmonic acid (JA) accumulation and highly induced the expression of chitinase, thus improving resistance to PWN. 3) The tolerance strategy involved the induction of phosphatidylcholine, which promoted flavonoid and anthocyanin synthesis via LPIN (phosphatidate phosphatase), LOX2S (lipoxygenase), and LOX1_5 (linoleate 9S-lipoxygenase), thus significantly inhibiting the pathogenicity of the PWN but not the PCA. These results illustrate the molecular mechanisms by whichP. massonianacultivars resist or tolerate PWN and are of great significance in the prevention and control of PWD.

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Section: Introductionmentioning

confidence: 99%

The molecular basis of pine wilt disease resistance inPinus massoniana

Wang,

Cai,

Zeng

et al. 2024

Preprint

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Application of Mutagenesis and Genome Editing in Crop Plants

Hussain,

Sagar,

Kapoor

et al. 2024

Sustainable Landscape Planning and Natural Resources Management

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Host Specificity of Soilborne Pathogens inHordeumSpecies and Their Relatives

Jin

Chen²,

Xu³

et al. 2023

Plant Disease

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Soil-borne pathogens destabilize the yields of Triticeae crops, including barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.). Although genetic resistance derived from relatives of these species has been utilized to prevent rust diseases (i.e. in the wheat-rye 1BL-1RS translocation line), research on resistance against soil-borne pathogens remains limited. Here, we performed field trials using 76 genotypes representing 28 Hordeum, six Triticum, and two Aegilops species to examine resistance against three soil-borne bymoviruses: barley yellow mosaic virus (BaYMV), barley mild mosaic virus (BaMMV), and wheat yellow mosaic virus (WYMV). We also performed greenhouse tests using the soil-borne fungal pathogen Fusarium pseudograminearum, which causes Fusarium crown rot (FCR). Using RT-PCR, we detected BaMMV and BaYMV in several Hordeum species, whereas WYMV induced systemic infection in the Triticum and Aegilops species. The identification of FCR susceptibility in all species examined suggests that F. pseudograminearum is a facultative fungal pathogen in Triticeae. Intra-species variation in FCR disease severity was observed for several species, pointing to the possibility of exploring host resistance mechanisms. Therefore, by unlocking the host specificity of four soil-borne pathogens in Hordeum species and their relatives, we obtained insights for the further exploration of wild sources of soil-borne pathogen resistance for future wheat and barley improvement programs.

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Mutation of barley HvPDIL5-1 improves resistance to yellow mosaic virus disease without growth or yield penalties

Cited by 3 publications

References 64 publications

The molecular basis of pine wilt disease resistance inPinus massoniana

The molecular basis of pine wilt disease resistance inPinus massoniana

Application of Mutagenesis and Genome Editing in Crop Plants

Host Specificity of Soilborne Pathogens inHordeumSpecies and Their Relatives

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