Mutation of an Arginine Biosynthesis Gene Causes Reduced Pathogenicity in <i>Fusarium oxysporum</i> f. sp. <i>melonis</i>

Namiki, Fumio; Matsunaga, Michiko; Okuda, Mitsuru; Inoue, Itaru; Nishi, Kazufumi; Fujita, Yoshikatsu; Tsuge, Takashi

doi:10.1094/mpmi.2001.14.4.580

Cited by 242 publications

(164 citation statements)

References 25 publications

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“…Plasmid pPN82 (9.7 kb), for the constitutive expression of GFP, was prepared by sequentially ligating into pBluescriptII KSþ a 0.7-kb BamHI/ SalI fragment of enhanced GFP (EGFP) from pEGFP (Clontech), a 1.4-kb HindIII fragment of hph under the control of the trpC promoter from pCB1004 (Carroll et al, 1994), a 2.3-kb EcoRI/NcoI fragment of gpd promoter from pAN7-1 (Punt et al, 1987), and a 0.6-kb NotI/SacII fragment of trpC terminator from pII99 (Namiki et al, 2001). The gpd promoter fragment was prepared by digesting a PCR product amplified with primer set M13-reverse and pgpdNc using pAN7-1 as template.…”

Section: Preparation Of Enhanced Gfp Vectormentioning

confidence: 99%

Reactive Oxygen Species Play a Role in Regulating a Fungus–Perennial Ryegrass Mutualistic Interaction

et al. 2006

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Although much is known about the signals and mechanisms that lead to pathogenic interactions between plants and fungi, comparatively little is known about fungus-plant mutualistic symbioses. We describe a novel role for reactive oxygen species (ROS) in regulating the mutualistic interaction between a clavicipitaceous fungal endophyte, Epichloe¨festucae, and its grass host, Lolium perenne. In wild-type associations, E. festucae grows systemically in intercellular spaces of leaves as infrequently branched hyphae parallel to the leaf axis. A screen to identify symbiotic genes isolated a fungal mutant that altered the interaction from mutualistic to antagonistic. This mutant has a single-copy plasmid insertion in the coding region of a NADPH oxidase gene, noxA. Plants infected with the noxA mutant lose apical dominance, become severely stunted, show precocious senescence, and eventually die. The fungal biomass in these associations is increased dramatically, with hyphae showing increased vacuolation. Deletion of a second NADPH oxidase gene, noxB, had no effect on the E. festucaeperennial ryegrass symbiosis. ROS accumulation was detected cytochemically in the endophyte extracellular matrix and at the interface between the extracellular matrix and host cell walls of meristematic tissue in wild-type but not in noxA mutant associations. These results demonstrate that fungal ROS production is critical in maintaining a mutualistic fungus-plant interaction.

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Section: Preparation Of Enhanced Gfp Vectormentioning

confidence: 99%

Reactive Oxygen Species Play a Role in Regulating a Fungus–Perennial Ryegrass Mutualistic Interaction

et al. 2006

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“…For the transformation experiments using geneticin (Sigma-Aldrich) as the selective agent, we transferred each gene cassette of Co ATG26, including the short promoter and terminator regions of SCD1, into pII99 carrying a geneticin resistance gene (Namiki et al, 2001). The gene cassettes released from pBATG26FL, pBATG26DCAT, pBATG26DPBD, and pBATG26CAT were introduced into pII99, which resulted in pIIATG26FL, pIIATG26DCAT, pIIATG26DPBD, and pIIATG26CAT, respectively.…”

Section: Plasmid Constructsmentioning

confidence: 99%

Atg26-Mediated Pexophagy Is Required for Host Invasion by the Plant Pathogenic Fungus Colletotrichum orbiculare

et al. 2009

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The number of peroxisomes in a cell can change rapidly in response to changing environmental and physiological conditions. Pexophagy, a type of selective autophagy, is involved in peroxisome degradation, but its physiological role remains to be clarified. Here, we report that cells of the cucumber anthracnose fungus Colletotrichum orbiculare undergo peroxisome degradation as they infect host plants. We performed a random insertional mutagenesis screen to identify genes involved in cucumber pathogenesis by C. orbiculare. In this screen, we isolated a homolog of Pichia pastoris ATG26, which encodes a sterol glucosyltransferase that enhances pexophagy in this methylotrophic yeast. The C. orbiculare atg26 mutant developed appressoria but exhibited a specific defect in the subsequent host invasion step, implying a relationship between pexophagy and fungal phytopathogenicity. Consistent with this, its peroxisomes are degraded inside vacuoles, accompanied by the formation of autophagosomes during infection-related morphogenesis. The autophagic degradation of peroxisomes was significantly delayed in the appressoria of the atg26 mutant. Functional domain analysis of Atg26 suggested that both the phosphoinositide binding domain and the catalytic domain are required for pexophagy and pathogenicity. In contrast with the atg26 mutant, which is able to form appressoria, the atg8 mutant, which is defective in the entire autophagic pathway, cannot form normal appressoria in the earlier steps of morphogenesis. These results indicate a specific function for Atg26-enhanced pexophagy during host invasion by C. orbiculare.

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“…No obstante, es conveniente reconocer que algunas cepas de F. oxysporum no poseen acción patogénica (Namiki et al, 2001) por lo que la patogenicidad específica del aislamiento obtenido en este estudio requiere de una caracterización detallada.…”

Section: Resultados Y Discusiónunclassified

AISLAMIENTO E IDENTIFICACIÓN DE HONGOS PATÓGENOS DE NARANJACitrus sinensisL. OSBECK CULTIVADA EN BAJA CALIFORNIA SUR, MÉXICO ISOLATION AND IDENTIFICATION OF PATHOGENIC FUNGI FROM ORANGECitrus sinensisL. OSBECK CULTURED IN BAJA CALIFORNIA SUR, MEXICO

Ochoa¹,

Hernández-Montiel²,

Latisnere-Barragán³

et al. 2007

Ciencia y Tecnologia Alimentaria

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Mutation of an Arginine Biosynthesis Gene Causes Reduced Pathogenicity in Fusarium oxysporum f. sp. melonis

Cited by 242 publications

References 25 publications

Reactive Oxygen Species Play a Role in Regulating a Fungus–Perennial Ryegrass Mutualistic Interaction

Reactive Oxygen Species Play a Role in Regulating a Fungus–Perennial Ryegrass Mutualistic Interaction

Atg26-Mediated Pexophagy Is Required for Host Invasion by the Plant Pathogenic Fungus Colletotrichum orbiculare

AISLAMIENTO E IDENTIFICACIÓN DE HONGOS PATÓGENOS DE NARANJACitrus sinensisL. OSBECK CULTIVADA EN BAJA CALIFORNIA SUR, MÉXICO ISOLATION AND IDENTIFICATION OF PATHOGENIC FUNGI FROM ORANGECitrus sinensisL. OSBECK CULTURED IN BAJA CALIFORNIA SUR, MEXICO

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