Mutation of alkyl hydroperoxide reductase gene ahpC of Xanthomonas oryzae pv. oryzae affects hydrogen peroxide accumulation during the rice–pathogen interaction

X, Li; Qiao, Jie; Yang, Liu; Qiao, Suyu; Pang, Xinyue; Tian, Fang; Chen, Huamin; He, Chenyang

doi:10.1016/j.resmic.2014.07.010

Cited by 2 publications

(3 citation statements)

References 26 publications

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“…In addition, the alkyl hydroperoxide reductase AhpC is also involved in the oxidative stress response. However, it has been reported that AhpC could only detoxify low concentrations of H 2 O 2 [ 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4

Yang

Shi

et al. 2017

PLoS ONE

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Agrobacterium tumefaciens GW4 is a heterotrophic arsenite [As(III)]/antimonite [Sb(III)]-oxidizing strain. The As(III) oxidase AioAB is responsible for As(III) oxidation in the periplasm and it is also involved in Sb(III) oxidation in Agrobacterium tumefaciens 5A. In addition, Sb(III) oxidase AnoA and cellular H2O2 are also responsible for Sb(III) oxidation in strain GW4. However, the deletion of aioA increased the Sb(III) oxidation efficiency in strain GW4. In the present study, we found that the cell mobility to Sb(III), ATP and NADH contents and heat release were also increased by Sb(III) and more significantly in the aioA mutant. Proteomics and transcriptional analyses showed that proteins/genes involved in Sb(III) oxidation and resistance, stress responses, carbon metabolism, cell mobility, phosphonate and phosphinate metabolism, and amino acid and nucleotide metabolism were induced by Sb(III) and were more significantly induced in the aioA mutant. The results suggested that Sb(III) oxidation may produce energy. In addition, without periplasmic AioAB, more Sb(III) would enter bacterial cells, however, the cytoplasmic AnoA and the oxidative stress response proteins were significantly up-regulated, which may contribute to the increased Sb(III) oxidation efficiency. Moreover, the carbon metabolism was also activated to generate more energy against Sb(III) stress. The generated energy may be used in Sb transportation, DNA repair, amino acid synthesis, and cell mobility, and may be released in the form of heat.

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Section: Resultsmentioning

confidence: 99%

Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4

Yang

Shi

et al. 2017

PLoS ONE

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“…Deletion of an alkyl hydroperoxide reductase gene ahpC in Xoo significantly decreased the endogenous H 2 O 2 accumulation. However, the H 2 O 2 scavenging activity was increased with a unknown compensatory mechanism, which led to a lower level of H 2 O 2 accumulation in the ahpC mutant than the wild type during their interactions with rice host plants [28]. These results indicated that AhpC plays the role in the detoxification of endogenous H 2 O 2 in Xoo .…”

Section: Discussionmentioning

confidence: 99%

“…Detoxification of endogenous H 2 O 2 that is generated through normal metabolic processes, such as aerobic respiration, is mediated by OxyR via regulating the ahpC and ahpF genes encoding alkyl hydroperoxide reductase [26, 27]. Deletion of ahpC significantly affected H 2 O 2 accumulation during the rice- Xoo interaction [28]. catB ( PXO_02830 ), katE ( PXO_02109 ) and srpA ( PXO_02864 ), three putative catalase genes were revealed in the genome of Xoo wildtype strain PXO99 A by in silico analysis [29].…”

Section: Introductionmentioning

confidence: 99%

OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae

Wang

MaoSen

et al. 2016

BMC Microbiol

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BackgroundTo facilitate infection, Xanthomonas oryzae pv. oryzae (Xoo), the bacterial blight pathogen of rice, needs to degrade hydrogen peroxide (H2O2) generated by the host defense response via a mechanism that is mediated by the transcriptional regulator OxyR. The catalase (CAT) gene catB has previously been shown to belong to the OxyR regulon in Xoo. However, its expression patterns and function in H2O2 detoxification and bacterial pathogenicity on rice remain to be elucidated.ResultsThe catB gene encodes a putative catalase and is highly conserved in the sequenced strains of Xanthomonas spp. β-galactosidase analysis and electrophoretic mobility shift assays (EMSA) showed that OxyR positively regulated the transcription of catB by directly binding to its promoter region. The quantitative real-time PCR (qRT-PCR) assays revealed that the expression levels of catB and oxyR were significantly induced by H2O2. Deletion of catB or oxyR drastically impaired bacterial viability in the presence of extracellular H2O2 and reduced CAT activity, demonstrating that CatB and OxyR contribute to H2O2 detoxification in Xoo. In addition, ΔcatB and ΔoxyR displayed shorter bacterial blight lesions and reduced bacterial growth in rice compared to the wild-type stain, indicating that CatB and OxyR play essential roles in the virulence of Xoo.ConclusionsTranscription of catB is enhanced by OxyR in response to exogenous H2O2. CatB functions as an active catalase that is required for the full virulence of Xoo in rice.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0887-0) contains supplementary material, which is available to authorized users.

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Mutation of alkyl hydroperoxide reductase gene ahpC of Xanthomonas oryzae pv. oryzae affects hydrogen peroxide accumulation during the rice–pathogen interaction

Cited by 2 publications

References 26 publications

Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4

Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4

OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae

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