Mutation of ACX1, a Jasmonic Acid Biosynthetic Enzyme, Leads to Petal Degeneration in Chinese Cabbage (Brassica campestris ssp. pekinensis)

Peng, Shenling; Huang, Shengnan; Li, Yong; Feng, Hui

doi:10.3390/ijms20092310

Cited by 12 publications

(9 citation statements)

References 73 publications

(95 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In wild tobacco, the floral limbs cannot fully open when JA biosynthesis is interrupted ( Stitz et al, 2014 ). A similar pattern is also found in Chinese cabbage ( Brassica campestris ; Peng et al, 2019 ) and rice ( Xiao et al, 2014 ). Recently, a study in tomato suggests that the JA-mediated flower development and opening are mediated by SlMYB21 ( Niwa et al, 2018 ).…”

Section: Introductionsupporting

confidence: 74%

Transcriptomic Profiling Reveals Shared Signalling Networks Between Flower Development and Herbivory-Induced Responses in Tomato

Wang

Schäfer

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Most flowering plants must defend themselves against herbivores for survival and attract pollinators for reproduction. Although traits involved in plant defence and pollinator attraction are often localised in leaves and flowers, respectively, they will show a diffuse evolution if they share the same molecular machinery and regulatory networks. We performed RNA-sequencing to characterise and compare transcriptomic changes involved in herbivory-induced defences and flower development, in tomato leaves and flowers, respectively. We found that both the herbivory-induced responses and flower development involved alterations in jasmonic acid signalling, suppression of primary metabolism and reprogramming of secondary metabolism. We identified 411 genes that were involved in both processes, a number significantly higher than expected by chance. Genetic manipulation of key regulators of induced defences also led to the expression changes in the same genes in both leaves and flowers. Targeted metabolomic analysis showed that among closely related tomato species, jasmonic acid and α-tomatine are correlated in flower buds and herbivory-induced leaves. These findings suggest that herbivory-induced responses and flower development share a common molecular machinery and likely have coevolved in nature.

show abstract

Section: Introductionsupporting

confidence: 74%

Transcriptomic Profiling Reveals Shared Signalling Networks Between Flower Development and Herbivory-Induced Responses in Tomato

Wang

Schäfer

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Collectively, our results indicate that cluster 0 belongs to PCs; cluster 1 belongs to MPCs; cluster 2 belongs to EMs; cluster 3 belongs to GCs; cluster 4 belongs to unknown (u.k.) cells; cluster 5 belongs to LMs; cluster 6 belongs to MMCs; cluster 7 belongs to YGCs; cluster 8 belongs to GMCs. Notably, the expression of some marker genes of the JA signal transduction pathway, such as ACYL-COA OXIDASE 1 ( ACX1 ) (Peng et al, 2019), ABNORMAL INFLORESCENCE MERISTEM ( AIM1 ) (Delker et al, 2007), BLADE ON PETIOLE1 ( BOP1 ) (Canet et al, 2012), CORONATINE INSENSITIVE 1 ( COI1 ) (Xie et al, 1998; Thines et al, 2007), CONSTITUTIVE EXPRESSION OF PR GENES 5 ( CPR5 ) (Clarke et al, 2001), CULLIN 1 ( CUL1 ) (Quint et al, 2005); JASMONATE-ZIM-DOMAIN PROTEIN 10 ( JAZ10 ) (Chung and Howe, 2009), JASMONATE-INDUCED OXYGENASE2 ( JAO2 ), JASMONATE-INDUCED OXYGENASE3 ( JAO3 ) (Caarls et al, 2017), PRODUCTION OF ANTHOCYANIN PIGMENT 1 ( PAP1 ) (Bali et al, 2019), RADICAL-INDUCED CELL DEATH1 ( RCD1 ) (Overmyer et al, 2000), and RIBONUCLEASE 1 ( RNS1 ) (LeBrasseur et al, 2002) was also significant in cluster 2, suggesting that they may function in EMs (Supplemental Figure S3).…”

Section: Resultsmentioning

confidence: 99%

Identification of two bZIP transcription factors that regulate development of pavement and trichome cells in Arabidopsis thaliana by single-cell RNA-sequencing

Liu

Wang

et al. 2022

Preprint

View full text Add to dashboard Cite

Epidermal cells are the main avenue for signal and material exchange between plants and the environment. Leaf epidermal cells primarily include pavement cells (PCs), guard cells, and trichomes cells (TCs), which differentiate from protodermal cells or meristemoids. The development and distribution of different epidermal cells are tightly regulated by a complex transcriptional regulatory network mediated by phytohormones, including jasmonic acid (JA), and transcription factors. Understanding how the fate of leaf epidermal cells is determined, however, is still largely unknown due to the diversity of cell types and the complexity of its regulation. Here, we characterized the transcriptional profiles of epidermal cells in 3-day-old true leaves of Arabidopsis thaliana using single-cell RNA-sequencing. We identified two genes encoding BASIC LEUCINE-ZIPPER (bZIP) transcription factors, namely the bZIP25 and bZIP53, which are highly expressed in PCs and early-stage meristemoid cells. Densities of PCs and TCs were found to increase and decrease, respectively, in bzip25 and bzip53 mutants, compared with wild-type plants. This trend was more pronounced in the presence of JA, suggesting that these transcription factors regulate the development of TCs and PCs in response to JA.IN A NUTSHELLBackgroundLeaf epidermal cells, comprised of trichome cells (TCs), guard cells (GCs), and pavement cells (PCs), are responsible for exchanging materials and information between plants and the surrounding aerial environment. Many genes have been identified in Arabidopsis thaliana and confirmed to be involved in the initiation and differentiation of TCs and PCs. The fate determination of TCs and PCs is tightly regulated by positive and negative regulators at the cellular level. The precise underlying molecular mechanisms responsible for the fate determination of TCs and PCs, however, are still unclear at this time.QuestionWhat are the transcriptomic profiles of different leaf epidermal cell types? Can we dissect the genes that are specifically expressed in certain epidermal cell types? What kinds of transcription factors are involved in regulating the fate determination of TCs and PCs?FindingsWe performed single cell RNA-seq to investigate the transcriptomic profiles of different leaf epidermal cell types and identified differentially expressed genes in each cell type. We found that genes that are involved in jasmonic acid signaling are highly expressed in early-stage meristemoid (EM) cells which can act as the precursor of PCs and perhaps of TCs. To investigate the regulatory mechanisms underlying EM development, we identified the transcription factors (TFs) in EM cells and found that two bZIP TF genes, bZIP25 and bZIP53, are highly expressed in EMs. Further analyses of these two genes using both loss-of-function and gain-of-function approaches indicated that bZIP25 and bZIP53 are functionally involved in promoting trichome formation but inhibit pavement cell development in response to jasmonic acid.Next stepsBesides of bZIP25 and bZIP53, we also identified other key genes, for example FES1B, in leaf epidermal cells. Our next step will be to explore the regulation of other key genes involved in the fate determination of different cell types in leaf epidermis.

show abstract

“…This is consistent with a previous report that the ectopic expression of AtJAZ1 lacking the Jas domain caused sterile pollen in Arabidopsis since the Jas domain is important for JA signaling transduction and protein interaction [ 75 ]. The JA biosynthesis gene ACX1 and DAD1 also affect the flower development and petal expansion in Chinese cabbage and Arabidopsis [ 71 , 73 , 75 , 81 ]. The function of PpJAZ1 -mediated cleistogamy in camelina will shed light on the potential application of the JAZ1 gene in the PMGF in plants.…”

Section: Discussionmentioning

confidence: 99%

Engineered Cleistogamy inCamelina sativafor bioconfinement

Huang

Gao

McAdams

et al. 2022

Horticulture Research

View full text Add to dashboard Cite

Summary Camelina sativa is a self-pollinating and facultative outcrossing oilseed crop. Genetic engineering has been used to improve camelina yield potential for altered fatty acid composition, modified protein profiles, improved seed and oil yield, and enhanced drought resistance. The deployment of transgenic camelina in the field posits high risks related to the introgression of transgenes into non-transgenic camelina and wild relatives. Thus, effective bioconfinement strategies need to be developed to prevent pollen-mediated gene flow (PMGF) from transgenic camelina. In the present study, we overexpressed the cleistogamy (i.e. floral petal non-openness)-inducing PpJAZ1 gene from peach in transgenic camelina. Transgenic camelina overexpressing PpJAZ1 showed three levels of cleistogamy, affected pollen germination rates after anthesis but not during anthesis, and caused a minor silicle abortion only on the main branches. We also conducted field trials to examine the effects of the overexpressed PpJAZ1 on PMGF in the field, and found that the overexpressed PpJAZ1 dramatically inhibited PMGF from transgenic camelina to non-transgenic camelina under the field conditions. Thus, the engineered cleistogamy using the overexpressed PpJAZ1 is a highly effective bioconfinement strategy to limit PMGF from transgenic camelina, and could be used for bioconfinement in other dicot species.

show abstract

Mutation of ACX1, a Jasmonic Acid Biosynthetic Enzyme, Leads to Petal Degeneration in Chinese Cabbage (Brassica campestris ssp. pekinensis)

Cited by 12 publications

References 73 publications

Transcriptomic Profiling Reveals Shared Signalling Networks Between Flower Development and Herbivory-Induced Responses in Tomato

Transcriptomic Profiling Reveals Shared Signalling Networks Between Flower Development and Herbivory-Induced Responses in Tomato

Identification of two bZIP transcription factors that regulate development of pavement and trichome cells in Arabidopsis thaliana by single-cell RNA-sequencing

Engineered Cleistogamy inCamelina sativafor bioconfinement

Contact Info

Product

Resources

About