Mutation in shoot-to-root mobile transcription factor, ELONGATED HYPOCOTYL 5, leads to low nicotine levels in tobacco

Singh, Deeksha; Singh, Hiteshwari; Singh, Nivedita; Dwivedi, Shweta; Trivedi, Prabodh Kumar

doi:10.1101/2022.01.05.475064

Cited by 5 publications

(9 citation statements)

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“…In a previous investigation, we explored the role of the light signaling component NtHY5 in nicotine biosynthesis. Our findings unveiled the positive regulatory function of NtHY5, as mutations in HY5 resulted in a significant decrease in nicotine biosynthesis and accumulation (Singh et al, 2023). However, the involvement of other light-associated networks has not been studied as yet.…”

Section: Introductionmentioning

confidence: 64%

“…In our recent study (Singh et al, 2023), we found that NtHY5 positively regulates nicotine biosynthesis, as evidenced by lower nicotine levels in NtHY5 CR plants. Building on this, in the current study, we developed CRISPR/Cas9-based NtCOP1 mutant lines in tobacco (Supplementary Fig.…”

Section: Ntcop1 Interact With Nthy5 To Antagonistically Regulate the ...mentioning

confidence: 75%

“…Extensive research has suggested role of COP1 as a repressor across various biological processes, including photomorphogenic development, flavonoid biosynthesis, embryo development, the transition from vegetative to reproductive phases, and anthocyanin biosynthesis, spanning plant species such as Arabidopsis thaliana, Oryza sativa, Pyrus bretschneideri, and Malus domestica (Li et al, 2013;Maier et al, 2012;Tanaka et al, 2011;Kim et al, 2017;Wu et al, 2019;Bhatia et al, 2021). While our previous work elucidated the role of NtHY5 in nicotine biosynthesis (Singh et al, 2023), there remains a gap in understanding the involvement of COP1 in the biosynthesis of these molecules. Addressing this gap, our study investigates and unveils the role of NtCOP1 in nicotine and flavonoid biosynthesis.…”

Section: Introductionmentioning

confidence: 85%

“…Earlier studies in Arabidopsis and other plant species have established HY5 as a master regulator of photomorphogenesis and a crucial signaling component in light response, promoting photomorphogenesis across a broad spectrum of wavelengths (Ulm et al, 2004;Lee et al, 2007;Nawkar et al, 2017). However, our recent study (Singh et al, 2023) focuses on the identification and functional characterization of tobacco HY5. In this study, we revealed that NtHY5 functions as a shoot-root mobile signal, orchestrating the light-regulated synthesis of nicotine in the root.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, we delineated the involvement of NtHY5 in light-mediated nicotine biosynthesis in tobacco (Singh et al, 2023). Given the pivotal role of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) in modulating HY5 activity through ubiquitination and subsequent degradation (Bhatia et al, 2021), it emerges as a central regulator in light signaling cascades.…”

Section: Identification and Homolog Analysis Of Ntcop1mentioning

confidence: 99%

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HY5 and COP1 function antagonistically in the light-dependent regulation of nicotine biosynthesis in tobacco

Singh,

Dwivedi,

Singh

et al. 2024

Preprint

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Nicotine constitutes approximately 90% of the total alkaloid content within theNicotianaspecies, rendering it the most prevalent alkaloid. While the majority of genes responsible for nicotine biosynthesis express in root tissue, the influence of light on this process through shoot-to-root mobile ELONGATED HYPOCOTYL 5 (HY5) has been recognized. CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), a key regulator of light-associated responses, known for its role in modulating HY5 accumulation, remains largely unexplored in its relationship to light-dependent nicotine accumulation. Here, we identified NtCOP1, a COP1 homolog inNicotiana tabacum, and demonstrated its ability to complement thecop1mutant inArabidopsis thalianaat molecular, morphological, and biochemical levels. Through the development of NtCOP1 overexpression (NtCOP1OX) plants, we observed a significant reduction in nicotine and flavonol content, inversely correlated with the down-regulation of nicotine and phenylpropanoid pathway. Conversely, CRISPR/Cas9-based knockout mutant plants (NtCOP1CR) exhibited an increase in nicotine levels. Further investigations, including yeast-two hybrid assays, grafting experiments, and western blot analyses, revealed that NtCOP1 modulates nicotine biosynthesis by targeting NtHY5, thereby impeding its transport from shoot-to-root. We conclude that the interplay between HY5 and COP1 functions antagonistically in the light-dependent regulation of nicotine biosynthesis in tobacco.HighlightCharacterization of CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) overexpressing and CRISPR/Cas9-based mutant plants suggests the intricate role of COP1 in modulating nicotine biosynthesis in tobacco.

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Section: Introductionmentioning

confidence: 64%

Section: Ntcop1 Interact With Nthy5 To Antagonistically Regulate the ...mentioning

confidence: 75%

Section: Introductionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Identification and Homolog Analysis Of Ntcop1mentioning

confidence: 99%

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HY5 and COP1 function antagonistically in the light-dependent regulation of nicotine biosynthesis in tobacco

Singh,

Dwivedi,

Singh

et al. 2024

Preprint

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Plant salt response: Perception, signaling, and tolerance

Xiao

Zhou

2023

Front. Plant Sci.

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Salt stress is one of the significant environmental stressors that severely affects plant growth and development. Plant responses to salt stress involve a series of biological mechanisms, including osmoregulation, redox and ionic homeostasis regulation, as well as hormone or light signaling-mediated growth adjustment, which are regulated by different functional components. Unraveling these adaptive mechanisms and identifying the critical genes involved in salt response and adaption are crucial for developing salt-tolerant cultivars. This review summarizes the current research progress in the regulatory networks for plant salt tolerance, highlighting the mechanisms of salt stress perception, signaling, and tolerance response. Finally, we also discuss the possible contribution of microbiota and nanobiotechnology to plant salt tolerance.

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Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in Dracaena cambodiana Response to Ultraviolet-B Radiation

Liang

Zhang

Zhu

et al. 2023

Forests

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Ultraviolet-B (UV-B) radiation is a major abiotic stress that dragon trees are exposed to during their growth and development; however, it is also an environmental signal perceived by plants that affects the flavonoid pathway. Previous studies have demonstrated that amounts of flavonoids are contained in dragon tree resin, otherwise known as dragon’s blood. However, the traits and mechanism involved in the UV-B-mediated increase in flavonoids in dragon trees are still unknown. Here, we studied the response of Dracaena cambodiana under full solar UV-B radiation. The results showed that the contents of total flavonoids in D. cambodiana significantly increased after UV-B radiation exposure. Then, the transcriptome was used for determining the interactive mechanism of flavonoid accumulation and UV-B stress. Differential expression analyses identified 34 differentially expressed genes (DEGs) involved in flavonoid synthesis; specifically, 24 of the identified DEGs were significantly up-regulated after UV-B radiation exposure. In addition, 57 DEGs involved in Ca2+/kinase sensors, 58 DEGs involved in ROS scavenging and the plant hormone pathway, and 116 DEGs transcription factors in 5 families were further identified and analyzed. Finally, we deduced the potential mechanism of UV-B-promoting flavonoid formation to neutralize ROS damage derived from UV-B radiation in D. cambodiana based on the gene co-expression network and previous studies from other plants. Considering that wild dragon tree populations are currently highly threatened by anthropogenic and natural stressors, the interactive studies between D. cambodiana plants and UV-B radiation provide valuable information toward understanding the mechanism of dragon’s blood formation and help us reveal the evolution of D. cambodiana, with the eventual goal of aiding in the global conservation of this precious biological resource.

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Mutation in shoot-to-root mobile transcription factor, ELONGATED HYPOCOTYL 5, leads to low nicotine levels in tobacco

Cited by 5 publications

References 158 publications

HY5 and COP1 function antagonistically in the light-dependent regulation of nicotine biosynthesis in tobacco

HY5 and COP1 function antagonistically in the light-dependent regulation of nicotine biosynthesis in tobacco

Plant salt response: Perception, signaling, and tolerance

Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in Dracaena cambodiana Response to Ultraviolet-B Radiation

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