Mutation in a chlorophyll-binding motif of Brassica ferrochelatase enhances both heme and chlorophyll biosynthesis

Liu, Mengyang; Ma, Wei; Su, Xiangjie; Zhang, Xiaomeng; Lu, Yin; Zhang, Shaowei; Yan, Jinghui; Feng, Dingqing; Ma, Lisong; Taylor, Aoife; Ge, Yunjia; Cheng, Qi; Xu, Kedong; Wang, Yanhua; Li, Na; Gu, Aiqin; Zhang, Ju; Luo, Sushan; Xuan, Shuxin; Chen, Xueping; Scrutton, Nigel S.; Li, Chengwei; Zhao, Jianjun; Shen, Shuxing

doi:10.1016/j.celrep.2022.111758

Cited by 11 publications

(15 citation statements)

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“…The phenomenon of anthocyanin accumulation induced by heme has also been reported in some plants [11]. VIT [12] and FC [13] are closely related to the contents of iron ions and heme in plant cells, respectively, and they may participate in anthocyanin metabolism in plants.…”

Section: Introductionmentioning

confidence: 73%

Deciphering the Anthocyanin Metabolism Gene Network in tea plant(Camellia sinensis) through Structural Equation modeling

Xia,

Chen,

Chen

et al. 2024

Preprint

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Background：Tea is an important cash crop that significantly contributes to rural development, poverty reduction and food security in many developing countries. It provides livelihoods for millions of smallholder producers and aids their economic stability. Anthocyanins in tea leaves provides excellent commercial quality and germplasm exploration potential. These compounds give tea leaves vibrant colors and increase health benefits. The current understanding of the synergistic regulation mechanisms responsible for color changes in purple tea, attributed to anthocyanin degradation, remains unclear. Results: In this study, we have identified 30 gene families within the tea genome that are associated related to with anthocyanin metabolism from tea genome. These gene families play distinct roles in the biosynthesis of anthocyanin including the formation of the core, structure, modification of the molecular framework, facilitation of transport process, regulation of gene expression, breakdown pathways, sugar transportation and iron ion respectively. The transcriptome expression pattern of gene families associated with anthocyanin accumulation in tea leaves of two varieties (N61 and Zikui) and natural development fading of these pigments in the tea leaves of one variety (ZJ). Subsequently, we investigated the synergistic mechanisms of anthocyanin metabolism related gene families within tea leaves using structural equation modeling. The results showed that sugar transport positively affects anthocyanin transportation, and promotes anthocyanin degradation during leaf pigmentation, whereas, it inhibits anthocyanin degradation during the fading of leaf color. Further, Iron ions facilitate the degradation of anthocyanins during their deposition and conversely, impede this degradation process during digestion. These finding suggests that tea plants may regulate the synthesis and degradation of anthocyanins through sugar transport and iron ions. And ensures healthy levels and vibrant colors. Conclusions：Our study contributes valuable information into the dynamic equilibrium anthocyanin mechanism and sheds light on complex regulatory mechanisms that govern the synthesis, transport and degradation of these pigments. These insights could be further used to develop strategies for enhancing anthocyanins content in unique tea germplasm to aid tea industry in producing new tea products with increased health benefits and aesthetic appeals.

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Section: Introductionmentioning

confidence: 73%

Deciphering the Anthocyanin Metabolism Gene Network in tea plant(Camellia sinensis) through Structural Equation modeling

Xia,

Chen,

Chen

et al. 2024

Preprint

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“…Under increasing N, the upregulated expression of this gene was observed in N36, and although both up/downregulation of this gene were observed in N60, it was less upregulated than in N36, which led us to speculate that the expression of a magnesium-chelating enzyme during N processing is beneficial for chlorophyll synthesis over heme synthesis. Similarly, Liu et al [ 41 ] found in the study of Brassica that the CAB domain in BrFC2 was not the structure to maintain the catalytic activity of heme enzyme, and only after the single base mutation of dBrFC2 could both increase the content of chlorophyll and heme in plants. It can be seen that N36 treatment is likely to change the domain of one or more genes in the chlorophyll synthesis of N .…”

Section: Discussionmentioning

confidence: 98%

“…Thus, we predicted that the expression levels of these two enzyme genes in N. tangutorum would increase with increased N addition. Previous reports have shown that the expression of ALA-synthesis-related genes can affect the chlorophyll contents of plants [ 41 ] and that GluTR can regulate ALA synthesis at the transcriptional level, thereby affecting chlorophyll synthesis [ 75 , 76 ]. Here, we obtained similar results and speculated that glutamyl tRNA reductase is likely to be a key regulatory site for ALA synthesis when N. tangutorum receive external N input.…”

Section: Discussionmentioning

confidence: 99%

“…The GATA58 gene of Glycine max can participate in the regulation of chlorophyll biosynthesis, and this regulation also responds to nitrogen addition [ 40 ]. Moreover, Sheng et al [ 41 ] found that ferrochelatase 2 ( BrFC 2 ), a single base mutation ( dBrFC2 ) involved in plant heme synthesis, can simultaneously improve the heme and chlorophyll content of Brassica . However, the identification of transcription factors involved in regulating chlorophyll biosynthesis is still limited to herbaceous plants and remains poor for N-treated woody plants.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Profiling and Chlorophyll Metabolic Pathway Analysis Reveal the Response of Nitraria tangutorum to Increased Nitrogen

Liu

Duan

Chen

et al. 2023

Plants

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To identify genes that respond to increased nitrogen and assess the involvement of the chlorophyll metabolic pathway and associated regulatory mechanisms in these responses, Nitraria tangutorum seedlings were subjected to four nitrogen concentrations (N0, N6, N36, and N60: 0, 6, 36, and 60 mmol·L−1 nitrogen, respectively). The N. tangutorum seedling leaf transcriptome was analyzed by high-throughput sequencing (Illumina HiSeq 4000), and 332,420 transcripts and 276,423 unigenes were identified. The numbers of differentially expressed genes (DEGs) were 4052 in N0 vs. N6, 6181 in N0 vs. N36, and 3937 in N0 vs. N60. Comparing N0 and N6, N0 and N36, and N0 and N60, we found 1101, 2222, and 1234 annotated DEGs in 113, 121, and 114 metabolic pathways, respectively, classified in the Kyoto Encyclopedia of Genes and Genomes database. Metabolic pathways with considerable accumulation were involved mainly in anthocyanin biosynthesis, carotenoid biosynthesis, porphyrin and chlorophyll metabolism, flavonoid biosynthesis, and amino acid metabolism. N36 increased δ-amino levulinic acid synthesis and upregulated expression of the magnesium chelatase H subunit, which promoted chlorophyll a synthesis. Hence, N36 stimulated chlorophyll synthesis rather than heme synthesis. These findings enrich our understanding of the N. tangutorum transcriptome and help us to research desert xerophytes’ responses to increased nitrogen in the future.

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“…Chlorophyll a-b binding proteins play an essential role in absorbing light and transferring energy [ 72 ]. In previous studies, Zm00001d033132 (lhcb12) was reported to be involved in the photosynthetic system response to low nitrogen supply and to be important for chlorophyll fluorescence [ 73 ]. Zm00001d021906 (lhca2) is important for maize to improve photosynthesis under abiotic stresses, such as drought and low temperature [ 74 , 75 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Transcriptome, Proteome, and Phosphoproteome Reveals Potential Roles of Photosynthesis Antenna Proteins in Response to Brassinosteroids Signaling in Maize

Gao

et al. 2023

Plants

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Brassinosteroids are a recently discovered group of substances that promote plant growth and productivity. Photosynthesis, which is vital for plant growth and high productivity, is strongly influenced by brassinosteroid signaling. However, the molecular mechanism underlying the photosynthetic response to brassinosteroid signaling in maize remains obscure. Here, we performed integrated transcriptome, proteome, and phosphoproteomic analyses to identify the key photosynthesis pathway that responds to brassinosteroid signaling. Transcriptome analysis suggested that photosynthesis antenna proteins and carotenoid biosynthesis, plant hormone signal transduction, and MAPK signaling in CK VS EBR and CK VS Brz were significantly enriched in the list of differentially expressed genes upon brassinosteroids treatment. Consistently, proteome and phosphoproteomic analyses indicated that photosynthesis antenna and photosynthesis proteins were significantly enriched in the list of differentially expressed proteins. Thus, transcriptome, proteome, and phosphoproteome analyses showed that major genes and proteins related to photosynthesis antenna proteins were upregulated by brassinosteroids treatment in a dose-dependent manner. Meanwhile, 42 and 186 transcription factor (TF) responses to brassinosteroid signals in maize leaves were identified in the CK VS EBR and CK VS Brz groups, respectively. Our study provides valuable information for a better understanding of the molecular mechanism underlying the photosynthetic response to brassinosteroid signaling in maize.

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Mutation in a chlorophyll-binding motif of Brassica ferrochelatase enhances both heme and chlorophyll biosynthesis

Cited by 11 publications

References 68 publications

Deciphering the Anthocyanin Metabolism Gene Network in tea plant(Camellia sinensis) through Structural Equation modeling

Deciphering the Anthocyanin Metabolism Gene Network in tea plant(Camellia sinensis) through Structural Equation modeling

Transcriptome Profiling and Chlorophyll Metabolic Pathway Analysis Reveal the Response of Nitraria tangutorum to Increased Nitrogen

Integrative Analysis of Transcriptome, Proteome, and Phosphoproteome Reveals Potential Roles of Photosynthesis Antenna Proteins in Response to Brassinosteroids Signaling in Maize

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