Mutation detection using Surveyor™ nuclease

Qiu, Peter; Shandilya, Harini; D'Alessio, James M.; O’Connor, Kevin; Durocher, Jeffrey A.; Gerard, Gary F.

doi:10.2144/04364pf01

Cited by 287 publications

(237 citation statements)

References 10 publications

(14 reference statements)

Supporting

Mentioning

225

Contrasting

Unclassified

Order By: Relevance

“…Technical progress, such as the procedure reported herein as well as advances in genome-wide screening technologies (40,41), should help in the future to identify more rapidly and more efficiently the preferential target molecules of individual antitumor responses. Their knowledge will allow to comprehensively study tumor-T cell interactions and to improve their therapeutic potential.…”

Section: Discussionmentioning

confidence: 99%

The response of autologous T cells to a human melanoma is dominated by mutated neoantigens

Lennerz

Fatho²,

Gentilini³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

411

318

View full text Add to dashboard Cite

Our understanding of pathways leading to antitumor immunity may depend on an undistorted knowledge of the primary antigenic targets of patients' autologous T cell responses. In the melanoma model derived from patient DT, we applied cryopreserved shortterm autologous mixed lymphocyte-tumor cell cultures (MLTCs) in combination with an IFN-␥ enzyme-linked immunospot (ELISPOT) assay to cDNA expression screening. We identified three previously unknown peptides processed from melanosomal proteins tyrosinase (presented by HLA-A*2601 and -B*3801) and gp100 (presented by HLA-B*07021) and five neoantigens generated by somatic point mutations in the patient's melanoma. The mutations were found in the genes SIRT2, GPNMB, SNRP116, SNRPD1, and RBAF600. Peptides containing the mutated residues were presented by HLA-A*03011, -B*07021, and -B*3801. Mutation-induced functional impairment was so far demonstrated for SIRT2. Within MLTC responder populations that were independently expanded from the patient's peripheral blood lymphocytes of different years, T cells against mutated epitopes clearly predominated. These results document a high degree of individuality for the cellular antitumor response and support the need for individualizing the monitoring and therapeutic approaches to the primary targets of the autologous T cell response, which may finally lead to a more effective cancer immunotherapy.expression cloning ͉ peptide ͉ tumor antigen ͉ mixed lymphocyte-tumor cell culture ͉ cytotoxic T lymphocytes

show abstract

Section: Discussionmentioning

confidence: 99%

The response of autologous T cells to a human melanoma is dominated by mutated neoantigens

Lennerz

Fatho²,

Gentilini³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

411

318

View full text Add to dashboard Cite

show abstract

“…There are several reports that EMC using CEL I nuclease demonstrates a very high sensitivity [70,73]. According to Qiu et al [71], the SURVEYOR Nuclease S corresponds to CEL II nuclease, a putative isozyme of CEL I nuclease [65]. EMC can be applied to longer PCR fragments compared to SSCP and HA [69,70].…”

Section: Discussionmentioning

confidence: 99%

“…Qiu et al analyzed 2.95-kb heteroduplex DNA by Surveyor nuclease [71]. In general, SSCP is thought to be most sensitive in detecting mutations when the fragment size is shorter than 200bp, and the size limit for high sensitivity is 300bp [1], even using CE-SSCP [12].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a simple and highly sensitive mutation screening system by enzyme mismatch cleavage with optimized conditions for standard laboratories

Tsuji

Niida

2008

Electrophoresis

View full text Add to dashboard Cite

Efficient screening of unknown DNA variations is one of the substantive matters of molecular biology even today. Historically, single-strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) are the most commonly used methods for detecting DNA variations everywhere in the world because of their simplicity. However, the sensitivity of these methods is not satisfactory for screening purpose. Recently, several new PCR based mutation screening methods have been developed, but most of them require special instruments and adjustment of conditions for each DNA sequence to attain the maximum sensitivity, eventually becoming as inconvenient as old methods. Enzyme mismatch cleavage (EMC) is potentially an ideal screening method. With high performance nucleases and once experimental conditions are optimized, it requires only conventional staff and conditions remain the same for each PCR product. In this study we tested four commercially available endonucleases for EMC and optimized the electrophoresis and developing conditions. We prepared 25 known DNA variations consisting of 18 single base substitutions (8 transitions and 10 transversions, including all possible sets of mismatches) and 7 small deletions or insertions. The combination of CEL nuclease, 12% polyacrylamide gel electrophoresis and rapid silver staining can detect all types of mutations and achieved 100% sensitivity.

show abstract

“…As shown in Fig. 5a, the principle of the SNP detection is based on cleavage activity of SURVEYOR nuclease which has been shown to recognize and cleave all types of SNP in heteroduplex DNA with high specificity (Qiu et al 2004). Following the immobilization of thiolated DNA probe molecules on the AuNRs surface, WT DNA or MT DNA containing SNP is introduced to bind with the DNA probe via DNA hybridization.…”

Section: Biosensing Detection Of Single Nucleotide Polymorphism Detecmentioning

confidence: 99%

Engineering plasmonic nanorod arrays for colon cancer marker detection

Dodson

Cao

Zaribafzadeh

et al. 2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Mutation detection using Surveyor™ nuclease

Cited by 287 publications

References 10 publications

The response of autologous T cells to a human melanoma is dominated by mutated neoantigens

The response of autologous T cells to a human melanoma is dominated by mutated neoantigens

Development of a simple and highly sensitive mutation screening system by enzyme mismatch cleavage with optimized conditions for standard laboratories

Engineering plasmonic nanorod arrays for colon cancer marker detection

Contact Info

Product

Resources

About