Mutation at residue 376 of ALS confers tribenuron-methyl resistance in flixweed (Descurainia sophia) populations from Hebei Province, China

Xu, Xiaogang; Liu, Guiqiao; Chen, Silong; Li, Binghua; Liu, Xiaomin; Wang, Xiaoyun; Cao, Fan; Wang, Guiqi; Ni, Hanwen

doi:10.1016/j.pestbp.2015.05.008

Cited by 21 publications

(14 citation statements)

References 34 publications

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“…In addition, tribenuron-methyl can be absorbed by roots, stems, leaves, and transfer in weeds. Flixweed populations across the country have evolved high level resistance to tribenuron-methyl, and ALS gene mutation at Pro197 or Asp376 was found to decrease the enzyme sensitivity, which is mainly responsible for resistance to tribenuron-methyl in flixweed [ 14 – 19 ]. However, NTSR mechanisms endowing tribenuron-methyl-resistance in flixweed have not previously investigated.…”

Section: Introductionmentioning

confidence: 99%

Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.)

Yang

Deng

et al. 2016

BMC Genomics

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BackgroundFlixweed (Descurainia sophia L.) is a troublesome and widespread broadleaf weed in winter fields in China, and has evolved high level resistance to acetolactate synthase (ALS)-inhibiting sulfonylurea herbicide tribenuron-methyl.ResultsWe identified a resistant flixweed population (N11) exhibiting 116.3-fold resistance to tribenuron-methyl relative to the susceptible population (SD8). Target-site ALS gene mutation Pro-197-Thr was identified in resistant plants. Moreover, the resistance can be reversed to 28.7-fold by the cytochrome P450 inhibitor malathion. The RNA-Sequencing was employed to identify candidate genes involved in non-target-site metabolic resistance in this population. Total 26 differentially expressed contigs were identified and eight of them (four P450s, one ABC transporter, three glycosyltransferase) verified by qRT-PCR. Consistent over-expression of the two contigs homology to CYP96A13 and ABCC1 transporter, respectively, were further qRT-PCR validated using additional plants from the resistant and susceptible populations.ConclusionsTribenuron-methyl resistance in flixweed is controlled by target-site ALS mutation and non-target-site based mechanisms. Two genes, CYP96A13 and ABCC1 transporter, could play an important role in metabolic resistance to tribenuron-methyl in the resistant flixweed population and justify further functional studies.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2915-8) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.)

Yang

Deng

et al. 2016

BMC Genomics

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“…We also confirmed tribenuron-methyl-resistant flixweed accessions with different Pro197 mutations exhibited different cross-resistance patterns to AHAS-inhibiting herbicides (Deng et al 2014). In this study, we identified and purified a flixweed assession (HB25) with an Asp-376-Glu mutation, which was firstly reported by Xu et al (2015). Therefore, there is no research on the cross-resistance patterns and biochemical mechanisms of flixweed accessions with Asp-376-Glu mutation to AHAS-inhibiting herbicides.…”

Section: Introductionmentioning

confidence: 68%

“…The amino acid at site of Pro197 could be substituted by Ala Leu, Ser, His, Thr, and Tyr in flixweed (Cui et al 2008(Cui et al , 2011Deng et al 2015). Recently, Xu et al (2015) indentified an Asp-376-Glu mutation in tribenuron-methyl-resistant flixweed accession, which was novel in flixweed. Indeed, the pHB25 accession carrying the same mutation, which was studied here, was collected and purified in our lab in 2014.…”

Section: Discussionmentioning

confidence: 99%

“…To date, the Asp-376-Glu mutation was identified only in plants of flix-weed, wild radish, false cleavers (Galium spurium), horseweed (Conyza canadensis), smooth pigweed (Amaranthus powellii), kochia (Kochia scoparia). The smooth pigweed (R11) and horseweed (P116) accessions, which carried an Asp-376-Glu mutation, exhibited high resistance to all four AHAS-inhibiting herbicides (Whaley et al 2007;Warwick et al 2008;Ashigh et al 2009;Zheng et al 2011;Beckie and Tardif 2012;Yu et al 2012;Xu et al 2015). There are many factors, which cause weeds evolve different cross-resistance patterns to AHAS-inhibiting herbicides, such as the weed species, AHAS structures, mutation sites and specific mutation amino acids.…”

Section: Discussionmentioning

confidence: 99%

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Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS

Deng

Yang

HongTao

et al. 2016

Journal of Integrative Agriculture

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Flixweed (Descurainia sophia L.) is a problematic and widespread weed in winter wheat fields and has been controlled by tribenuron-methyl for more than twenty years in China. In this study, a flixweed accession (HB25) with an Asp-376-Glu mutation in acetohydroxy acid synthase (AHAS) was identified and purified. The purified HB25 accession (pHB25) developed 758.1-fold resistance to tribenuron-methyl and exhibited obvious cross-resistance to four AHAS-inhibiting herbicides. The R/S ratios of GR 50 to herbicides of halosulfuron-methyl (SU), flumetsulam (TP), imazethapyr (IMI) and pyribenzoxim (PTB) were 346.1 15.7, 8.1 and 7.1, respectively. The reduced AHAS sensitivities to four different AHAS-inhibiting herbicides, which were caused by the Asp-376-Glu mutation, were responsible for the resistance and cross-resistance to AHAS-inhibiting herbicides. The R/S ratios of I 50 to tribenuron-methyl, halosulfuron-methyl, flumetsulam, imazethapyr and pyribenzoxim were 844.5, 532.9, 74.5, 13.3 and 5.5 respectively. The results of AHAS activity in vitro were highly correlated with that of whole-plant response experiments.

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“…Tribenuron-methyl (TBM), an SU herbicide, was produced by the Dupont Company in the early 1980s and introduced to China in 1988 [16]. At present, TBM is used in broadleaf weed control across wheat fields in China and accounts for over half of the total herbicide usage due to its high efficacy at low dosage, low effect on nontarget organisms and high selectivity [17]. However, conventional rapeseed varieties are sensitive to TBM due to the lack of resistant genes.…”

Section: Introductionmentioning

confidence: 99%

Inheritance and Molecular Characterization of a Novel Mutated AHAS Gene Responsible for the Resistance of AHAS-Inhibiting Herbicides in Rapeseed (Brassica napus L.)

Huang

Sun

et al. 2020

IJMS

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The use of herbicides is an effective and economic way to control weeds, but their availability for rapeseed is limited due to the shortage of herbicide-resistant cultivars in China. The single-point mutation in the acetohydroxyacid synthase (AHAS) gene can lead to AHAS-inhibiting herbicide resistance. In this study, the inheritance and molecular characterization of the tribenuron-methyl (TBM)-resistant rapeseed (Brassica napus L.) mutant, K5, are performed. Results indicated that TBM-resistance of K5 was controlled by one dominant allele at a single nuclear gene locus. The novel substitution of cytosine with thymine at position 544 in BnAHAS1 was identified in K5, leading to the alteration of proline with serine at position 182 in BnAHAS1. The TBM-resistance of K5 was approximately 100 times that of its wild-type ZS9, and K5 also showed cross-resistance to bensufuron-methyl and monosulfuron-ester sodium. The BnAHAS1544T transgenic Arabidopsis exhibited higher TBM-resistance than that of its wild-type, which confirmed that BnAHAS1544T was responsible for the herbicide resistance of K5. Simultaneously, an allele-specific marker was developed to quickly distinguish the heterozygous and homozygous mutated alleles BnAHAS1544T. In addition, a method for the fast screening of TBM-resistant plants at the cotyledon stage was developed. Our research identified and molecularly characterized one novel mutative AHAS allele in B. napus and laid a foundation for developing herbicide-resistant rapeseed cultivars.

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Mutation at residue 376 of ALS confers tribenuron-methyl resistance in flixweed (Descurainia sophia) populations from Hebei Province, China

Cited by 21 publications

References 34 publications

Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.)

Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.)

Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS

Inheritance and Molecular Characterization of a Novel Mutated AHAS Gene Responsible for the Resistance of AHAS-Inhibiting Herbicides in Rapeseed (Brassica napus L.)

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