Mutation Analysis of KRAS and BRAF Genes in Metastatic Colorectal Cancer: a First Large Scale Study from Iran

koochak, Aghigh; Rakhshani, Naser; Niya, Mohammad Hadi Karbalaie; Tameshkel, Fahimeh Safarnezhad; Sohrabi, Masoudreza; Babaee, Mohammad Reza; Rezvani, Hamid; Bahar, Burhanuddin; Imanzade, Farid; Zamani, Farhad; Khonsari, Mohammad Reza; Ajdarkosh, Hossein; Hemmasi, Gholamreza

doi:10.7314/apjcp.2016.17.2.603

Cited by 17 publications

(18 citation statements)

References 38 publications

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“…BRAF belongs to the family of RAF proteins and is an intracellular effector of the mitogen-activated protein kinase-signaling cascade ( 55 ). The BRAF V600E mutation is a molecular marker that has been reported in thyroid carcinoma ( 56 – 64 ). BRAF mutation rate was previously reported to be 29–83% in PTC, 37.5–77% in PTMC and 0.2–5.7% in benign thyroid disease ( 19 , 33 – 35 , 65 – 68 ).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic significance of CK19, galectin-3, CD56, TPO and Ki67 expression and BRAF mutation in papillary thyroid carcinoma

et al. 2018

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The aim of the present study was to examine the rate of BRAF mutation and the expression profiles of CK19, galectin-3, CD56, thyroid peroxidase (TPO) and Ki67 in papillary thyroid carcinoma (PTC) and papillary thyroid micro-carcinoma (PTMC). A total of 246 cases of thyroid disease were collected, including PTC, PTMC, nodular goiter (NG) and Hashimoto thyroiditis (HT). The results revealed that CK19 expression was 116/120 in PTC, 61/64 in PTMC, 2/34 in NG and 1/28 in HT. Galectin-3 positive expression was 115/120 in PTC, 60/64 in PTMC, 6/34 in NG and 4/28 in HT. TPO positive expression was 8/120 in PTC, 1/64 in PTMC, 30/34 in NG and 25/28 in HT. CD56-positive expression was 12/120 in PTC, 3/64 in PTMC, 33/34 in NG and 26/28 in HT. Ki67 labeling index was 2.52±0.46% in PTC (120 cases), 2.62±0.52% in PTMC (64 cases), 2.55±0.44% in NG (34 cases) and 2.58±0.48% in HT (28 cases). BRAF mutation rate was 93/120 in PTC, 47/64 in PTMC, 3/34 in NG and 2/28 in HT. These results suggested that expression patterns of CK19, galectin-3, CD56 and TPO and BRAF mutation exhibit diagnosis value in thyroid disease. However, Ki67-positive rate exhibits no notable diagnosis value in thyroid disease.

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Section: Discussionmentioning

confidence: 99%

Diagnostic significance of CK19, galectin-3, CD56, TPO and Ki67 expression and BRAF mutation in papillary thyroid carcinoma

et al. 2018

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“…A thin 20-μm tissue section was obtained from the blocks, and deparaffinization was performed with xylene. Then, series of distilled water and graded ethanol solutions were used for rehydration, according to previous works [2] , [24] , [25] . The DNA purification was performed by using QIAamp DNA FFPE Tissue Kit (QIAGEN, Hilden, Germany), according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

Human Papillomavirus Type 16 Integration Analysis by Real-time PCR Assay in Associated Cancers

Niya

Keyvani

Tameshkel

et al. 2018

Translational Oncology

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Human papillomavirus (HPV) is a common viral infection worldwide associated with a variety of cancers. The integration of the HPV genome in these patients causes chromosomal instability and triggers carcinogenesis. The aim of this study was to investigate the HPV-16 genome physical status in four major cancers related to HPV infection. Formalin-fixed paraffin-embedded blocks from our previous projects on head and neck, colorectal, penile, and cervical cancers were collected, and HPV-16–positive specimens were used for further analysis. The DNA extraction copy number of E2 and E7 genes was calculated by qualitative real-time PCR method. Serially diluted standards that were cloned in PUC57 plasmid were used. Standard curve and melting curve analysis was used for quantification. Of the 672 specimens studied, 76 (11.3%) were HPV-16 positive. We found that 35.6% (16/45) were integrated. Statistical analysis showed that there were significant correlations between integration of HPV-16 and cervical cancer end-stage carcinogenesis (P < .0001), episomal form, and ASCUS lesions (P = .045). Significant correlation in penile cancer patients was seen between the episomal form and high-grade cancer stage (P = .037). Integration is a major factor in the carcinogenesis mechanism of HPV and has different prevalence in various cancers with a higher rate in progression except in penile cancer.

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“…KRAS mutation was observed in 33.6% of CRC cases, which involves mutations at codon 12 and 13. There was a significant correlation between KRAS mutations and older ages and tumor type (non-mucinous) [68]. Incidence of mutation in codons 12 and 13 of KRAS was screened by another group among Iranian CRC subjects, showed that the codon 13 mutations had lower frequencies compared with codon 12 (8% vs. 24%).…”

Section: Main Textmentioning

confidence: 99%

Genetic and molecular origins of colorectal Cancer among the Iranians: an update

Abbaszadegan

Moghbeli

2018

Diagn Pathol

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BackgroundColorectal cancer (CRC) is one the leading causes of cancer related deaths among Iranians. Despite the various progresses in new therapeutic methods, it has still a low rate of survival. This high ratio of mortality is mainly related to the late diagnosis, in which the patients refer for treatment in advanced stages of tumor.Main bodycolorectal cancer progression is largely associated with molecular and genetic bases. Although Iran has a high ratio of CRC mortality, there is not an efficient genetic panel for detection and prognosis. Therefore, it is critical to introduce new diagnostic markers with ability to detect in early stages.ConclusionPresent review summarizes all of the genetic and epigenetic factors which are reported in CRC until now among the Iranian patients to pave the way of incorporation of new ethnic specific markers into the clinical practice and development of new targeted therapeutic methods.

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Mutation Analysis of KRAS and BRAF Genes in Metastatic Colorectal Cancer: a First Large Scale Study from Iran

Cited by 17 publications

References 38 publications

Diagnostic significance of CK19, galectin-3, CD56, TPO and Ki67 expression and BRAF mutation in papillary thyroid carcinoma

Diagnostic significance of CK19, galectin-3, CD56, TPO and Ki67 expression and BRAF mutation in papillary thyroid carcinoma

Human Papillomavirus Type 16 Integration Analysis by Real-time PCR Assay in Associated Cancers

Genetic and molecular origins of colorectal Cancer among the Iranians: an update

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