Mutants of the Saccharomyces cerevisiae VPS genes CCZ1 and YPT7 are blocked in different stages of sporulation

Piekarska, Iga; Kucharczyk, Róża; Mickowska, Barbara; Rytka, Joanna; Rempoła, Bożenna

doi:10.1016/j.ejcb.2010.06.009

Cited by 17 publications

(14 citation statements)

References 47 publications

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“…Third, it is tempting to speculate that the granular structures that were observed in TA-treated sporulating yeast (Figure 3A) are undigested autophagosomes. Fourth, a recent study showed that the deletion of CCZ1 , a gene important for both sporulation and autophagy, exhibits phenotypes similar to those observed in TA-treated yeast undergoing sporulation: while the meiotic transcriptional program and pre-meiotic DNA synthesis were largely unchanged, the meiotic divisions were strongly inhibited [53]. Finally, we found that the neo1 Δ /NEO1 heterozygous strain is highly sensitized to TA in sporulating cultures.…”

Section: Discussionsupporting

confidence: 70%

Cationic Amphiphilic Drugs Are Potent Inhibitors of Yeast Sporulation

et al. 2012

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Meiosis is a highly regulated developmental process that occurs in all eukaryotes that engage in sexual reproduction. Previous epidemiological work shows that male and female infertility is rising and environmental factors, including pollutants such as organic solvents, are thought to play a role in this phenomenon. To better understand how organic compounds interfere with meiotic development, the model organism Saccharomyces cerevisiae was exposed to 446 bioactive molecules while undergoing meiotic development, and sporulation efficiency was quantified employing two different high-throughput assays. 12 chemicals were identified that strongly inhibited spore formation but did not interfere with vegetative growth. Many of these chemicals are known to bind to monoamine-receptors in higher eukaryotes and are cationic amphiphilic drugs. A detailed analysis of one of these drugs, tripelennamine, revealed that it induces sporulation-specific cytotoxicity and a strong inhibition of meiotic M phase. The drug, however, only mildly interfered with pre-meiotic DNA synthesis and the early meiotic transcriptional program. Chemical-genomic screening identified genes involved in autophagy as hypersensitive to tripelennamine. In addition, we found that growing and sporulating yeast cells heterozygous for the aminophospholipid translocase, NEO1, are haploinsufficient in the presence of the drug.

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Section: Discussionsupporting

confidence: 70%

Cationic Amphiphilic Drugs Are Potent Inhibitors of Yeast Sporulation

et al. 2012

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“…In addition, whether meiotic autophagy functions are conserved in other organisms is another important issue. Critical roles of autophagy in meiosis appear to be conserved in yeast species (Piekarska et al 2010). However, meiosis-specific defects have not been reported in autophagy-defective mice (Mizushima & Levine 2010), suggesting that meiotic autophagy functions are not conserved in mammals.…”

Section: Discussionmentioning

confidence: 99%

Autophagy is required for efficient meiosis progression and proper meiotic chromosome segregation in fission yeast

Matsuhara

Yamamoto

2015

Genes to Cells

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Autophagy is a conserved intracellular degradation system, which contributes to development and differentiation of various organisms. Yeast cells undergo meiosis under nitrogen-starved conditions and require autophagy for meiosis initiation. However, the precise roles of autophagy in meiosis remain unclear. Here, we show that autophagy is required for efficient meiosis progression and proper meiotic chromosome segregation in fission yeast. Autophagydefective strains bearing a mutation in the autophagy core factor gene atg1, atg7, or atg14 exhibit deformed nuclear structures during meiosis. These mutant cells require an extracellular nitrogen supply for meiosis progression following their entry into meiosis and show delayed meiosis progression even with a nitrogen supply. In addition, they show frequent chromosome dissociation from the spindle together with spindle overextension, forming extra nuclei. Furthermore, Aurora kinase, which regulates chromosome segregation and spindle elongation, is significantly increased at the centromere and spindle in the mutant cells. Aurora kinase down-regulation eliminated delayed initiation of meiosis I and II, chromosome dissociation, and spindle overextension, indicating that increased Aurora kinase activity may cause these aberrances in the mutant cells. Our findings show a hitherto unrecognized relationship of autophagy with the nuclear structure, regulation of cell cycle progression, and chromosome segregation in meiosis.

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“…S9A and table S4; (14)]. ATG8 , a gene central to many branches of autophagy (15), was highly expressed from early in the meiotic program, and its deletion caused an early and profound meiotic defect (fig.…”

Section: Evidence For Cellular Remodelingmentioning

confidence: 99%

High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling

Brar

Yassour

Friedman

et al. 2012

Science

509

736

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Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of both mRNA levels and translational efficiency. Monitoring of protein production timing revealed uncharacterized recombination factors and extensive organellar remodeling. Meiotic translation is also shifted toward noncanonical sites, including short open reading frames (ORFs) on unannnotated transcripts and upstream regions of known transcripts (uORFs). Ribosome occupancy at near-cognate uORFs was associated with more efficient ORF translation; in contrast, some AUG uORFs, often exposed by regulated 5′ leader extensions, acted competitively. This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells.

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Mutants of the Saccharomyces cerevisiae VPS genes CCZ1 and YPT7 are blocked in different stages of sporulation

Cited by 17 publications

References 47 publications

Cationic Amphiphilic Drugs Are Potent Inhibitors of Yeast Sporulation

Cationic Amphiphilic Drugs Are Potent Inhibitors of Yeast Sporulation

Autophagy is required for efficient meiosis progression and proper meiotic chromosome segregation in fission yeast

High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling

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