Mutants of
            <i>Rhodospirillum rubrum</i>
            Obtained After Long-Term Anaerobic, Dark Growth

Uffen, R L; Sybesma, Christiaan; Wolfe, R. S.

doi:10.1128/jb.108.3.1348-1356.1971

Cited by 38 publications

(30 citation statements)

References 18 publications

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“…Dark, respiring Gl cells formed nonpigmented colonies; dark, fermenting cells developed light-pink colonies. As reported earlier (44), only a fraction of mutant Gl cells in anaerobic, dark cultures grew in light. Similarly, under conditions inside the anaerobic glove chamber, about one out of 10' Gl cells grew in light and formed deep-red (photosynthesizing) colonies ( Table 1, experiment 7).…”

Section: Resultssupporting

confidence: 79%

“…Organisms and growth media. R. rubrum mutants Gl and C were used (44). In anaerobic, darkgrown cultures of mutant C a small fraction of the cells formed nonpigmented colonies similar to mutant Gi.…”

Section: Methodsmentioning

confidence: 99%

“…Cells were grown in a complex medium with sodium pyruvate as described earlier (44,45). Liquid 'Published as journal article no.…”

Section: Methodsmentioning

confidence: 99%

“…In some experiments, sodium pyruvate was replaced by 0.3 g of sodium malate per 100 ml of medium, or different amounts of sodium acetate plus sodium pyruvate were used. Liquid medium for fermentation studies was prepared by adding 0.02 g of sodium thioglycollate (Difco) per 100 ml of distilled water instead of cysteine-sulfide reducing agent (44). The same liquid medium described earlier (45) for aerobic growth was modified by addition of 0.5 g of sodium pyruvate per 100 ml of medium.…”

Section: Methodsmentioning

confidence: 99%

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Growth Properties of Rhodospirillum rubrum Mutants and Fermentation of Pyruvate in Anaerobic, Dark Conditions

Uffen

1973

J Bacteriol

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Mutant C and G1 were obtained earlier from Rhodospirillum rubrum S 1 during growth in the dark under strict anaerobic conditions in medium containing sodium pyruvate. Mutant C and mutant G1 grew in the dark with generation times of 5.8 h and 4.6 h, respectively. Mutant C cells grew equally well when switched between anaerobic (dark or light) or aerobic, dark conditions. Mutant G1 cells grew only in the dark (anaerobic or aerobic conditions), but a fraction of cells in anaerobic, dark cultures grew when placed in light. This number increased about 3,000-fold when G1 cells were incubated aerobically in the dark. During anaerobic, dark growth, C and G1 organisms incorporated similar amounts of [2- 14 C ]sodium pyruvate. About 34% of the incorporated radioactivity was found in lipid fractions from C cells that developed chromatophores during dark growth. Similar results were obtained using G1 cells, which formed only trace amounts of photosynthetic structures. Both mutants fermented sodium pyruvate and produced acetate, formate, carbon dioxide, and hydrogen gas. Molar growth yield coefficients indicated that the cells obtained about 1 mol of adenosine triphosphate per mol of sodium pyruvate fermented. Results suggested that pyruvate fermentation during dark growth occurred via a pyruvate formate-lyase or the pyruvate ferredoxin-oxidoreductase pathway, or both.

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Section: Resultssupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

“…Cells were grown in a complex medium with sodium pyruvate as described earlier (44,45). Liquid 'Published as journal article no.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Growth Properties of Rhodospirillum rubrum Mutants and Fermentation of Pyruvate in Anaerobic, Dark Conditions

Uffen

1973

J Bacteriol

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“…Nitrogenase activity was stimulated by the addition of ferredoxin, indicating the presence of a pyruvate-dependent ferredoxin reductase. Finally, mutants with altered pigmentation accumulate after long-term anaerobic growth in the dark, and these mutants appear to metabolize pyruvate differently from the wild-type [114,118].…”

Section: Fermentatite Carbon Metabolismmentioning

confidence: 99%

Biochemical genetics revisited: the use of mutants to study carbon and nitrogen metabolism in the photosynthetic bacteria

Willison¹

1993

FEMS Microbiology Letters

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The biochemical genetics approach is defined as the use of mutants, in comparative studies with the wild‐type, to obtain information about biochemical and physiological processes in complex metabolic systems. This approach has been used extensively, for example in studies on the bioenergetics of the photosynthetic bacteria, but has been applied less frequently to studies of intermediary carbon and nitrogen metabolism in phototrophic organisms. Several important processes in photosynthetic bacteria—the regulation of nitrogenase synthesis and activity, the control of intracellular redox balance during photoheterotrophic growth, and chemotaxis—have been shown to involve metabolism. However, current understanding of carbon and nitrogen metabolism in these organisms is insufficient to allow a complete understanding of these phenomena. The purpose of the present review is to give an overview of carbon and nitrogen metabolism in the photosynthetic bacteria, with particular emphasis on work carried out with mutants, and to indicate areas in which the biochemical genetics approach could be applied successfully. In particular, it will be argued that, in the case of Rhodobacter capsulatus and Rb. sphaeroides, two species which are fast‐growing, possess a versatile metabolism, and have been extensively studied genetically, it should be possible to obtain a complete, integrated description of carbon and nitrogen metabolism, and to undertake a qualitative and quantitative analysis of the flow of carbon and reducing equivalents during photoheterotrophic growth. This would require a systematic biochemical genetic study employing techniques such as HPLC, NMR, and mass spectrometry, which are briefly discussed. The review is concerned mainly with Rb. capsulatus and Rb. sphaeroides, since most studies with mutants have been carried out with these organisms. However, where possible, a comparison is made with other species of purple non‐sulphur bacteria and with purple and green sulphur bacteria, and recent literature relevant to these organisms has been cited.

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Vergärung von Pyruvat durch sieben Arten phototropher Purpurbakterien

Gürgün¹,

Kirchner²,

Pfennig³

1976

J of Basic Microbiology

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zum 80. Geburtstag gewidmet (Eingegangen a m 3.2.1976) The dark, anaerobic fermentation of pyruvate under growth conditions was examined with the following species of phototrophic purple bacteria : Rhodospirillum rubrum strains Ha and S1, Rhodopseudomonas gelatinosa strain 2150, Rhodopseudomonas acidophila strain 7050, Rhodopseudomonas palustris strain ATCC 17001, Rhodopseudomonas capsulata strains K b l and 6950, Rhodopseudomonas sphaeroides strain ATCC 17023, and Chromatium vinosum strain D. Fermentation balances were established for all experiments.Under fermentative conditions cell protein and dry weight increased only slightly, if a t all.

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Mutants of Rhodospirillum rubrum Obtained After Long-Term Anaerobic, Dark Growth

Cited by 38 publications

References 18 publications

Growth Properties of Rhodospirillum rubrum Mutants and Fermentation of Pyruvate in Anaerobic, Dark Conditions

Growth Properties of Rhodospirillum rubrum Mutants and Fermentation of Pyruvate in Anaerobic, Dark Conditions

Biochemical genetics revisited: the use of mutants to study carbon and nitrogen metabolism in the photosynthetic bacteria

Vergärung von Pyruvat durch sieben Arten phototropher Purpurbakterien

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