Mutant Mouse L‐Cells: A Model for Megaloblastic Anaemia

Dardick, Irving; Sheinin, Rose; Setterfield, G.

doi:10.1111/j.1365-2141.1978.tb03617.x

Cited by 8 publications

(1 citation statement)

References 32 publications

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“…Impairment of DNA chain elongation [25] and of histone biosynthesis [26] have been postulated to cause failure of condensation of nuclear chromatin. Whatever be the underlying biochemical mechanism, the failure of condensation of nuclear chromatin (incomplete contraction or des piralization of chromosomes) may account for the at tenuated reticulated pattern of the nuclear chromatin, as well as for the finely dispersed, lacy appearance of nuclear profile of megaloblasts both under the light and the electron microscope [26,27].…”

Section: Discussionmentioning

confidence: 99%

Cytogenetics in Nutritional Megaloblastic Anaemia: Prolonged Persistence of Chromosomal Abnormalities in Lymphocytes after Remission

Das¹,

Mohanty²,

Garewal³

1986

Acta Haematol

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Chromosomal studies were performed in phytohaemagglutinin-stimulated cultures of lymphocytes and in bone marrow cells without culture from 115 patients with megaloblastic anaemia resulting from nutritional deficiency of folate and vitamin B₁₂ Essentially similar chromosomal abnormalities were observed in the two cell lines. These were characterized by striking morphological aberrations such as elongation and despiralization (uncoiling or incomplete contraction), increased frequency of chromosome breakage and centromere spreading. Numerical abnormalities, chromatid exchanges and translocations were virtually absent. Autoradiographic studies of chromosomes after pulse-labelling with ³H-thymidine during the terminal 6 h of phytohaemagglutinin-stimulated lymphocyte cultures revealed a differential pattern of distribution of the radionucleotide in the chromosomes of megaloblastic lymphocytes as compared to those from normal lymphocytes. Repeated chromosomal studies were done in 65 of these patients at various intervals after starting therapy with the deficient vitamins. After 48 h of therapy, chromosomal abnormalities were remarkably reduced in the bone marrow; but many of these morphological chromosomal changes (i.e. despiralization and breaks) persisted in the lymphocytes of a significant proportion of patients for variable periods up to 6–12 months after haematological remission resulting from therapy with the deficient vitamins. These abnormalities did not, however, persist after remission in those patients who had repeated episodes of infections.

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Section: Discussionmentioning

confidence: 99%

Cytogenetics in Nutritional Megaloblastic Anaemia: Prolonged Persistence of Chromosomal Abnormalities in Lymphocytes after Remission

Das¹,

Mohanty²,

Garewal³

1986

Acta Haematol

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Ultrastructural morphometric image analysis applied to estimation of condensed chromatin in normal and neoplastic lymphocytes

Dardick¹,

Dardick²,

Jeans³

et al. 1985

J. Elec. Microsc. Tech.

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Currently, quantitative studies of malignant lymphoma are being performed in an attempt to improve the classification of non-Hodgkin's lymphoma (NHL) for diagnostic, prognostic, and therapeutic purposes. Morphometric image analysis is one method that can be employed in cases of NIIL to obtain objective data of nuclear parameters; condensed chromatin being a compartment of the nucleus best measured at the ultrastructural level. This report assesses similarities or differences in the amount, distribution, and arrangement of condensed chromatin in nuclear profiles of normal and neoplastic lymphocytes in human surgical biopsy specimens. Morphometric data derived from electron micrographs of lymphocytes in germinal centers of lymph nodes with reactive hyperplasia (three cases) and small cell types of NHL (two examples of malignant lymphoma, well differentiated lymphocytic type (ML,WDL) and three cases of malignant lymphoma, poorly differentiated lymphocytic type (ML, PDL)) are compared. Results indicate that the distribution of condensed chromatin, i.e., the size of aggregates, and their spatial placement within the nucleus varies more than the amount 030th n e a n area per profile or mean volume) of this nuclear parameter, and that this applies to normal as well as neoplastic lymphocytes. When a series of condensed chromatin parameters were statistically compared, no major differences could be detected between lymphocytes in normal tissues and those in ML,WDL and ML,PDL, but considerable differences were found in each of the nuclear morphotypes in the individual cases within the groups. This degree of variation in nuclear characteristics within normal tissues and the two lymphoma categories has not been previously recognized. Clearly, the technique of morphometric analysis, as applied to electron micrographs, can provide new and useful data that must be appreciated if classification schemes currently used in NHL are to improve _ * and reflect biologic considerations.

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Temperature-Sensitive Mutants in the Study of Cell Cycle Progression in Mammalian Cells

Sheinin¹

1980

Nuclear-Cytoplasmic Interactions in the Cell Cycle

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Mutant Mouse L‐Cells: A Model for Megaloblastic Anaemia

Cited by 8 publications

References 32 publications

Cytogenetics in Nutritional Megaloblastic Anaemia: Prolonged Persistence of Chromosomal Abnormalities in Lymphocytes after Remission

Cytogenetics in Nutritional Megaloblastic Anaemia: Prolonged Persistence of Chromosomal Abnormalities in Lymphocytes after Remission

Ultrastructural morphometric image analysis applied to estimation of condensed chromatin in normal and neoplastic lymphocytes

Temperature-Sensitive Mutants in the Study of Cell Cycle Progression in Mammalian Cells

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