Mutant Mice and Animal Models of Airway Allergic Disease

Brassard, Julyanne; Marsolais, David; Blanchet, Marie‐Renée

doi:10.1007/978-1-0716-1095-4_6

Cited by 2 publications

(2 citation statements)

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“…Thickening of the bronchial wall, and swelling of airway epithelium, could also be found in the OVA group. The aforementioned infiltration was suppressed by ART, with a lower HE scores compared to the OVA-treated group ( Brassard et al, 2021 ) ( Figures 4A, B ). Meanwhile, mice with OVA showed a significantly obvious mucus production in epithelial layers.…”

Section: Resultsmentioning

confidence: 94%

“…The degree of inflammatory cell infiltration surrounding the tiny airways was used to quantify the level of inflammation, which was rated on a scale from 0 to 4. The criteria ( Brassard et al, 2021 ) were as follows: 0, no cells; 1, a little inflammatory cell infiltration around the airway; 2, 1-2 layers of inflammatory cells infiltrating around the airway; three points, 3-5 layers of infiltration; 4, more than five layers of infiltration. Inflammation scores were completed by one investigator in a single-blind manner.…”

Section: Methodsmentioning

confidence: 99%

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Artesunate inhibits airway remodeling in asthma via the MAPK signaling pathway

Zhang

Lin²,

Zhang³

et al. 2023

Front. Pharmacol.

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Background: Artesunate (ART), is a semi-synthetic water-soluble artemisinin derivative extracted from the plant Artemisia annua, which is often used to treating malaria. In vivo and in vitro studies suggested it may help decrease inflammation and attenuate airway remodeling in asthma. However, its underlying mechanism of action is not elucidated yet. Herein, an attempt is made to investigate the ART molecular mechanism in treating asthma.Methods: The BALB/c female mice sensitized via ovalbumin (OVA) have been utilized to establish the asthma model, followed by carrying out ART interventions. Lung inflammation scores by Haematoxylin and Eosin (H&E), goblet cell hyperplasia grade by Periodic Acid-Schiff (PAS), and collagen fibers deposition by Masson trichrome staining have been utilized for evaluating how ART affected asthma. RNA-sequencing (RNA-seq) analyses were performed to identify differentially expressed genes (DEGs). The DEGs were analyzed by Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and Protein-Protein interaction (PPI) function analyses. Hub clusters were found by Cytoscape MCODE. Subsequently, Real-Time quantitative PCR (RT-qPCR) verified the mRNA expression profiles of DEGs. Finally, immunohistochemistry (IHC) and western blots have validated the relevant genes and potential pathways.Results: ART considerably attenuated inflammatory cell infiltration, mucus secretion, and collagen fibers deposition. KEGG pathway analysis revealed that the ART played a protective role via various pathways including the mitogen-activated protein kinase (MAPK) pathway as one of them. Moreover, ART could alleviate the overexpression of found in inflammatory zone 1(FIZZ1) as revealed by IHC and Western blot analyses. ART attenuated OVA-induced asthma by downregulating phosphorylated p38 MAPK.Conclusion: ART exerted a protective function in a multitarget and multi-pathway on asthma. FIZZ1 was a possible target for asthma airway remodeling. The MARK pathway was one of the key pathways by which ART protected against asthma.

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Section: Resultsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%