Mutant analysis, a key tool for the study of metabolism and development

Cove, David J.

doi:10.1111/j.1365-313x.1993.tb00181.x

Cited by 6 publications

(6 citation statements)

References 14 publications

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“…The characterization of nit mutants [Cove, 1976[Cove, , 1993 and their use for studying fungal populations allowed Joaquim and Rowe [1990] to identify only four VCGs, among 22 V. dahliae isolates previously assigned to 15 VCGs by Puhalla and Hummel [2983]. Subsequent work [Strausbaugh et al, 1992] has also revealed only four VCGs (1, 2, 4, and 5) in V. dahliae.…”

Section: Discussionmentioning

confidence: 99%

Differentiation ofVerticillium dahliae populations on the basis of vegetative compatibility and pathogenicity on cotton

1995

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Complementary auxotrophic nitrate-nonutilizing (nit) mutants were used to investigate vegetative compatibility within 27 strains of Verticillium dahliae isolated from several hosts originating from Africa, Asia, Europe and the United States. Using about 500 nit mutants generated from these strains, three vegetative compatibility groups, 1, 2 and 4, were identified. Simultaneously, virulence of each strain was assessed on cultivars of Gossypium hirsutum, G. barbadense and G. arboreum, based upon Foliar Alteration Index (FAI) and Browning Index (BI) estimation. The strains in VCG1 were of both the cottondefoliating pathotype and race 3 (on cotton) but were non pathogenic on tomato; those in VCG2 and VCG4 were of the nondefoliating pathotype and belonged to different races on cotton and on tomato. Hyaline mutants deriving from parental wild-type strain showed differences in pathogenicity but were always assigned to the parental VCG. A relationship was established between VCGs and the taxonomic position of host plants. Data from nit pairings indicated that the sub-populations of V. dahliae (VCGs) may not be completely isolated genetically.

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Section: Discussionmentioning

confidence: 99%

Differentiation ofVerticillium dahliae populations on the basis of vegetative compatibility and pathogenicity on cotton

1995

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“…Usefulness of such mutants has also been proven in current molecular biology, especially for isolation and characterization of plant genes (Sussex, 1989;Cove, 1993). It is, however, not always easy to obtain a mutant affecting a certain character.…”

Section: Introductionmentioning

confidence: 99%

Specific developmental stages of gametogenesis for radiosensitivity and mutagenesis in rice

et al. 1994

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Developmental stages during gametogenesis of rice were histologically examined in the period from differentiation of reproductive organs to anthesis. Plants were exposed to acute X-rays of 20 Gy. Radiosensitivity and mutation frequency were investigated in relation to the developmental stages of reproductive organs. The most radiosensitive stage, as measured by reduction of the M1 pollen-and seed-fertilities, was the last premeiotic interphase. Mutations induced at different developmental stages were scored in M3 strains. Sterility mutants and short-culm mutants were most frequently observed. Grain shape, panicle morphology, heading-date and endosperm character mutants were induced at a relatively low frequency. The overall mutation frequency varied with the developmental stage at the time of irradiation. The highest overall mutation frequency was observed when radiation was applied 10 days before anthesis, the late tetrad stage of microspores. Radiation exposure of florets at the late tetrad stage was found to be a more efficient method of inducing a large number of mutations than radiations applied to seeds and fertilized egg cells.

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“…In general, single-gene mutants represent useful tools for studying biological processes providing material for more detailed molecular analyses (Cowe, 1993). Epi is an ethylene overproducer tomato mutant that displays leaf epinasty, stem thickening and constitutive triple response in dark-grown seedlings resembling an ethylenetreated wild type plant (Fujino et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Supraoptimal ethylene acts antagonistically with exogenous gibberellins during Solanum lycopersicum (Solanaceae) hypocotyl growth.

Mignolli¹,

Rojas²,

Vidoz³

2016

Bol. Soc. Argent. Bot.

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In many plant species, ethylene and gibberellins interact to regulate plant growth and development. In some cases, these hormones can act in a synergistic way whereas in others they can be antagonistic. To date, the control of hypocotyl elongation by ethylene and gibberellins has been poorly explored in tomato. In this paper, we report that, application of exogenous ethylene to tomato seedlings or high endogenous ethylene production, as in the epinastic mutant, strongly prevent the effect of gibberellic acid (GA3) application. Moreover, constitutive activation of gibberellin signal in a DELLA deficient mutant is not able to counteract the inhibitory effect of ethylene on hypocotyl elongation, suggesting that ethylene acts independently from DELLA-mediated gibberellin response. Interestingly, when ethylene perception is blocked, the GA3 promotive effect on hypocotyl length is less effective, indicating that the presence of a basal level of ethylene could synergistically enhance hypocotyl growth. Taken together, these observations may suggest that, in tomato, supraoptimal concentrations of ethylene are able to antagonize gibberellin effect but normal levels seem to promote gibberellin-induced hypocotyl elongation.

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Mutant analysis, a key tool for the study of metabolism and development

Cited by 6 publications

References 14 publications

Differentiation ofVerticillium dahliae populations on the basis of vegetative compatibility and pathogenicity on cotton

Differentiation ofVerticillium dahliae populations on the basis of vegetative compatibility and pathogenicity on cotton

Specific developmental stages of gametogenesis for radiosensitivity and mutagenesis in rice

Supraoptimal ethylene acts antagonistically with exogenous gibberellins during Solanum lycopersicum (Solanaceae) hypocotyl growth.

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