Mutagensensitivität von Patienten mit Larynx- bzw. Oropharynxkarzinomen1

Kleinsasser, N.; Ua, Harréus; Bc, Wallner; Er, Kastenbauer

doi:10.1055/s-1999-8767

Cited by 7 publications

(4 citation statements)

References 5 publications

(6 reference statements)

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“…Moreover, supplementation by antioxidants results to an increased resistance to oxidative damage of lymphocytes obtained from healthy subjects (25). Antioxidants may also prevent smoke-induced DNA single-strand breaks in mouse organs (26).…”

Section: Discussionmentioning

confidence: 99%

Effects of cigarette smoking on the antioxidant defence in young healthy male volunteers

Charalabopoulos

Assimakopoulos

Karkabounas

et al. 2004

International Journal of Clinical Practice

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Cigarette smoking induces a significant oxidant effect related to variety of free radical-related diseases often affecting the upper respiratory tract, unless it is effectively compensated by the antioxidant barriers of the humans. In the present study, the evaluation of the antioxidant compensatory mechanisms, by estimating the antioxidant capacity of extracellular defence (saliva and plasma) and the intracellular resistance of peripheral lymphocytes to oxidative stress in young healthy smokers, was investigated. Twenty young healthy male smokers and 20 age-matched non-smokers with similar dietary profiles were enrolled in the study. Total saliva and plasma samples were collected from both groups, and total antioxidant capacity (TAC) and lag time were estimated. The latter was also repeated in smokers just after a cigarette smoking. Peripheral lymphocytes isolated from the subjects of both groups were also tested for their inherent DNA damage as well as for their ability to resist H2O2-induced DNA damage by using the comet assay. TAC of plasma was found significantly higher in smokers compared to non-smokers (p <0.004), whereas no difference was recorded in plasma lag time values. Lymphocytes of smokers manifested a significantly decreased oxidant resistance (increased DNA fragmentation) to H2O2, in comparison to non-smokers. Our results indicate that young smokers do not manifest different salivary antioxidant defence than non-smokers. They exhibit, however, a higher plasma antioxidant capacity, but a significantly reduced ability of blood lymphocytes, to resist to H2O2-induced DNA damage.

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Section: Discussionmentioning

confidence: 99%

Effects of cigarette smoking on the antioxidant defence in young healthy male volunteers

Charalabopoulos

Assimakopoulos

Karkabounas

et al. 2004

International Journal of Clinical Practice

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“…Um den DNA-Schaden zu quantifizieren, wird das Ausmaß der DNA-Migration mit dem Olive-Tailmoment beschrieben [11]. Einzelheiten zu Material und Methodik können früheren Veröffentlichungen an dieser Stelle entnommen werden [9,25,29].…”

Section: Der Mikrokerntestunclassified

“…Auch die DNA-Schädigung durch Insektizide wie Pentachlorphenol und Lindan [22] sowie Genotoxizitäts-und Zytotoxizitätsstudien an Olaflur wurden an oropharyngealer Schleimhaut und an Lymphozyten mit dem Comet Assay durchgeführt [23]. Aufgrund der unterschiedlichen Ergebnisse von einzelnen Spendern in den ersten Untersuchungen an humaner Schleimhaut [9,20] [25,26]. Auch andere Messungen an laryngealer Schleimhaut von Larynxkarzinompatienten ergaben erhöhte Schädigungsraten verglichen mit Spendern ohne Malignom [27].…”

Section: Diskussionunclassified

Stellenwert der Einzelzell Mikrogelelektrophorese in der Ökogenotoxikologie

Ua¹,

Er²,

Bc³

et al. 2002

Laryngorhinootologie

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The microgel electrophoresis technique is a sensitive method to detect genotoxic effects and DNA repair in human epithelia of the upper aerodigestive tract. The assay offers considerable advantages to other common genotoxicity tests. However, combining of the Comet assay with mini organ cultures allows to use repetitive incubations with xenobiotics. Furthermore, signalling selected chromosomal material by the combination of the assay with the fluorescence in situ hybridisation, DNA-damage and -repair mechanisms within comets can be identified.

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“…Ohne gegenüber zusätzlichen Noxen im Akuttest zu ex− ponieren, kann der Assay außerdem zur Beurteilung von chroni− schen Beanspruchungen der Individuen in vivo als Testverfahren im Sinne eines Biomonitorings eingesetzt werden[91]. Darüber hinaus gibt er über spezifische Empfindlichkeiten gegenüber Schadstoffen, die so genannte Mutagensensitivität, Auskunft[82,92,93]. Damit ist dieses Testverfahren ebenfalls ex vivo ein− setzbar.…”

unclassified

Toxikologische Bewertung von Inhalationsnoxen: Testmethoden, Einschätzung der toxischen Wirksamkeit und des Gefahrenpotenzials, Grenzwerte

2004

Laryngorhinootologie

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Mutagensensitivität von Patienten mit Larynx- bzw. Oropharynxkarzinomen1

Cited by 7 publications

References 5 publications

Effects of cigarette smoking on the antioxidant defence in young healthy male volunteers

Effects of cigarette smoking on the antioxidant defence in young healthy male volunteers

Stellenwert der Einzelzell Mikrogelelektrophorese in der Ökogenotoxikologie

Toxikologische Bewertung von Inhalationsnoxen: Testmethoden, Einschätzung der toxischen Wirksamkeit und des Gefahrenpotenzials, Grenzwerte

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