Mutagenicity evaluation of metal oxide nanoparticles by the bacterial reverse mutation assay

Pan, Xiaoping; Redding, James E.; Wiley, Patricia A.; Wen, Lisa; McConnell, J. S.; Zhang, Baohong

doi:10.1016/j.chemosphere.2009.12.056

Cited by 139 publications

(76 citation statements)

References 22 publications

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“…These results were in agreement with those reported by other authors, which till now have recorded no mutagenicity potential of different nanosize metal oxides (Al 2 O 3 , Co 3 O 4 , TiO 2 , ZnO, and Fe 3 O 4 ) to S. typhimurium and E. coli, both in the absence and presence of metabolic activation (Pan et al 2010;Pereira et al 2011;Lopes et al 2012;Szalay et al 2012). However, although the nano-TiO 2 tested in our study was smaller (<25 nm) than those used in previous studies, in Milli Q water (dilution medium in the Ames test), this nanooxide formed aggregates higher than 500 nm in diameter (Table 2), and this could have not favored the cellular uptake and the access to genetic material.…”

Section: Discussionsupporting

confidence: 95%

Assessing the ecotoxicity of metal nano-oxides with potential for wastewater treatment

Nogueira

Lopes

Rocha-Santos

et al. 2015

Environ Sci Pollut Res

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The rapid development of nanotechnology and the increasing use of nanomaterials (NMs) raise concern about their fate and potential effects in the environment, especially for those that could be used for remediation purposes and that will be intentionally released to the environment. Despite the remarkable emerging literature addressing the biological effects of NMs to aquatic organisms, the existing information is still scarce and contradictory. Therefore, aimed at selecting NMs for the treatment of organic and inorganic effluents, we assessed the potential toxicity of NiO (100 and 10-20 nm), Fe2O3 (≈85 × 425 nm), and TiO2 (<25 nm), to a battery of aquatic organisms: Vibrio fischeri, Raphidocelis subcapitata, Lemna minor, Daphnia magna, Brachionus plicatilis, and Artemia salina. Also a mutagenic test was performed with two Salmonella typhimurium strains. Suspensions of each NM, prepared with the different test media, were characterized by dynamic light scattering (DLS) and eletrophoretic light scattering (ELS). For the assays with marine species, no toxicity was observed for all the compounds. In opposite, statistically significant effects were produced on all freshwater species, being D. magna the most sensitive organism. Based on the results of this study, the tested NMs can be classified in a decreasing order of toxicity NiO (100 nm) > NiO (10-20 nm) > TiO2 (<25 nm) > Fe2O3, allowing to infer that apparently Fe2O3 NMs seems to be the one with less risks for receiving aquatic systems.

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Section: Discussionsupporting

confidence: 95%

Assessing the ecotoxicity of metal nano-oxides with potential for wastewater treatment

Nogueira

Lopes

Rocha-Santos

et al. 2015

Environ Sci Pollut Res

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“…The available data indicate that CuONPs are mutagenic and cause DNA strand breaks in bacteria such as Escherichia coli and Salmonella typhimurium [5,11]. In vitro studies indicate that CuONPs can induce DNA damage, DNA strand breaks and chromosome alterations in human lung epithelial, skin, peripheral blood and cancer cell lines [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 96%

Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

Carmona

Inostroza‐Blancheteau

Obando

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…58 ZnO NPs (,100 nm) were negative in the Ames test up to 1000 µg/mL in the absence of S9 metabolic activation and induced only marginal mutagenesis in Escherichia coli WP2 trp uvrA in the presence of 9% S9 fraction. 59 Tetramethylammonium hydroxide-coated ZnO NPs (size before coating was reported to be 5.4 nm) were negative in the Ames test using Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537 and E. coli strain WP2uvrA(-), with and without metabolic activation using S9 pre-incubation. 60 ZnO NPs (,100 nm) induced genotoxicity in HEp-2 human cervix carcinoma cells, using the Comet assay and the cytokinesis-blocked micronucleus assay.…”

Section: Genotoxicity Studies In Vivo and In Vitro In Vivo Studiesmentioning

confidence: 99%

A review of mammalian toxicity of ZnO nanoparticles

Vandebriel¹,

Jong²

2012

NSA

435

247

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This review summarizes the literature on mammalian toxicity of ZnO nanoparticles (NPs) published between 2009 and 2011. The toxic effects of ZnO NPs are due to the compound's solubility. Whether the increased intracellular [Zn 2+ ] is due to the NPs being taken up by cells or to NP dissolution in medium is still unclear. In vivo airway exposure poses an important hazard. Inhalation or instillation of the NPs results in lung inflammation and systemic toxicity. Reactive oxygen species (ROS) generation likely plays an important role in the inflammatory response. The NPs do not, or only to a minimal extent, cross the skin; this also holds for sunburned skin. Intraperitoneal administration induces neurological effects. The NPs show systemic distribution; target organs are liver, spleen, lung, and kidney and, in some cases, the heart. In vitro exposure of BEAS-2B bronchial epithelial cells and A549 alveolar adenocarcinoma cells results in cytotoxicity, increased oxidative stress, increased intracellular [Ca 2+ ], decreased mitochondrial membrane potential, and interleukin (IL)-8 production. Decreased contractility of airway smooth muscle cells poses an additional hazard. In contrast to the results for BEAS-2B and A549 cells, in RKO colon carcinoma cells ZnO NPs and not Zn 2+ induce cytotoxicity and mitochondrial dysfunction. Short-term exposure of skin cells results in apoptosis but not in an inflammatory response, while long-term exposure leads to increased ROS generation, decreased mitochondrial activity, and formation of tubular intercellular structures. Macrophages, monocytes, and dendritic cells are affected; exposure results in cytotoxicity, oxidative stress, intracellular Ca 2+ flux, decreased mitochondrial membrane potential, and production of IL-1β and chemokine CXCL9. The NPs are phagocytosed by macrophages and dissolved in lysosomes. In vitro the Comet assay and the cytokinesis-blocked micronucleus assay show genotoxicity, whereas the Ames test does not. This is, however, not confirmed by in vivo genotoxicity assays. Protein binding results in increased stability.

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Mutagenicity evaluation of metal oxide nanoparticles by the bacterial reverse mutation assay

Cited by 139 publications

References 22 publications

Assessing the ecotoxicity of metal nano-oxides with potential for wastewater treatment

Assessing the ecotoxicity of metal nano-oxides with potential for wastewater treatment

Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

A review of mammalian toxicity of ZnO nanoparticles

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