Mutagenic treatment of microspore‐derived embryos of turnip rape (Brassica rapa) to increase oleic acid content

Abstract

The article presents the results of a study on obtaining mutant doubled haploids of turnip rape. The culture medium for producing embryos in isolated microspore culture was optimized. The optimal medium formula was NLN + 13% S + 0.05 mg/L NAA + 0.05 mg/L BA. Embryos derived from isolated microspore culture were treated with EMS mutagen in three concentrations (4 mM, 8 mM, and 12 mM). Mutant doubled haploid plants and their seeds were obtained. Agronomic evaluation and analysis of fatty acid composition in mutant lines seeds showed that they had valuable traits in terms of seed oil quality and yield. Analysis of the fatty acid composition of the seeds of the obtained mutant doubled haploids showed an increase in the percentage of oleic acid (~11%–12%) in comparison with donor cultivars. Our results showed that mutagenesis of embryos from a culture of isolated microspores has potential for improving the qualitative traits of turnip rape.

The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

“…Recently, Kitashiba et al (2016) have identified loci associated with embryo yield in microspore culture of Brassica rapa. Daurova et al changed microspore-derived embryos (MDE) fatty acid composition (Daurova et al, 2020). Genes associated with the induction and the establishment of embryogenesis in isolated microspores were studied by Malik et al (2007), and Tsuwamoto et al (2007).…”