Mutagenic Potential of a 193-nm Excimer Laser on Fibroblasts in Tissue Culture

Excimer laser "corneal shaping": a new technique for customized trephination in penetrating keratoplasty

“…The positive results on corneal wound healing were confirmed by other authors [3,14,16,33]. There is also ample evidence that excimer laser irradiation does not have a relevant mutagenic potential on corneal or adjacent tissue [12,27,36]. Since we used a commercially available excimer laser with only slightly increased pulse energy, a clinical effect on other ocular structures than the cornea during irradiation is unlikely.…”

Excimer laser treatment of corneal surface pathology: a laboratory and clinical study.

“…19 21 Finally the putative mutagenic effects of ultraviolet radiation on the cornea have been assessed by both unscheduled DNA repair and tissue culture, coupled with enzyme poisoning techniques. 22 23 None of these studies has highlighted areas of concern that would preclude the beginning of clinical trials with the excimer laser at 193 nm (Sliney D, personal communication).…”

Transmission of 308 nm Excimer Laser Radiation for Ophthalmic Microsurgery — Medical, Technical and Safety Aspects - Übertragung gepulster 308 nm Excimer-Laser-Strahlung für mikrochirurgische Anwendungen in der Augenheilkunde - medizinische, technische und Sicherheitsaspekte

“…The mutagenic Potential of 308 nm radiation is small äs compared to shorter UV wavelengths [49]. Irradiation of mouse f ibroblasts did not induce significant mutagenic effects [42,57]. Mutagenesis experiments using 193 nm excimer laser radiation have led to contradictary results.…”