Mutagenic Effect on Seed Germination, Seedling Growth and Seedling Survival of Pigeon Pea (&lt;i&gt;Cajanus cajan&lt;/i&gt; (L.) Millsp)

Ariraman, M.; Gnanamurthy, S.; Dhanavel, D.; Bharathi, T. Usha; Murugan, S. Pazhani Bala

doi:10.56431/p-g8xcn6

Cited by 6 publications

(5 citation statements)

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“…Further these reductions are caused due to that the seed absorbs the mutagen that subsequently reaching the meristem region and impact the germ cell line (Serrat et al, 2014). Same observation has been reported by Ariramana et al (2014), Kumar and Mishra (2004) and Baghery et al (2016).…”

Section: Resultsmentioning

confidence: 57%

Assessment on Seedling Traits of Black Gram (Vigna mungo L. Hepper) Mutants Raised during M2 Generation

Nayak,

Naik,

Padiya

et al. 2023

IJEP

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The present study was conducted at department of Genetics, Osmania University, Hyderabad, Telangana, India, during September to November, 2021 with the blackgram accession- IC-436524 obtained from NBPGR regional centre, Hyderabad, Telangana, India and the check T9 was collected from ICAR-CRIDA Hyderabad. The present study aimed to estimate the mutagenic sensitivity of black gram accession- IC-436524 with Ethyl methane sulfonate. Accordingly, this study checked whether the mutagenic activity has been inherited to the next generation M2. Data analysis has been performed for the determination of significance in germination percentage, seedling length (cm), seedling vigor index, root length (cm) and seed survival rate (Day 1 to Day 15) that were observed to be highly significant (p<0.01) for above characters except root length (cm) for treatments. It clearly indicated that there was adequate amount of variability among the black gram mutant due to treatments. The collected data for 7th day observed that seed germination percentage was higher at 0.2% and 0.3% EMS treated mutants and lowest germination percentage was observed in 0.5% EMS treated mutants. Seedling length (cm) was recorded highest in 0.2% EMS treated mutants and lowest seedling length (cm) was recorded in control. Maximum seedling vigor index was recorded in 0.2% EMS treated mutants and lowest seedling vigor index was showed for 0.5% EMS treated mutants. Seedling survival rate data was recorded for 15th day showed higher in 0.2% EMS treated mutants. Data showed EMS has induced mutations during M1 generation and inherited to offspring (M2 generation).

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Section: Resultsmentioning

confidence: 57%

Assessment on Seedling Traits of Black Gram (Vigna mungo L. Hepper) Mutants Raised during M2 Generation

Nayak,

Naik,

Padiya

et al. 2023

IJEP

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“…Earlier research work has shown that in okra ( Abelmoschus esculentus ), germination percentage generally decreased with increasing dose concentrations of gamma rays and the EMS levels ( Gupta et al, 2016 ). Reduced germination percentage with increasing doses of gamma radiation has also been reported in pinus ( Ariraman et al, 2014 ), rye ( Khah & Verma, 2015 ), and chickpea plants ( Shah et al, 2008 ). A gradual reduction in germination percentage was also observed with an increase in the concentration of mutagen, reaching more than 50% lethality at 0.5% EMS level in two genotypes of tobacco plants ( Dhakshanamoorthy, Selvaraj & Chidambaram, 2010 ).…”

Section: Discussionmentioning

confidence: 85%

“…In the greenhouse, the seedlings were irrigated with distilled water. After two weeks, the shoot and root lengths were measured using the sandwich blotter technique ( Ariraman et al, 2014 ). After sown on the nursery bed, the emergence was recorded for each dose after germination.…”

Section: Methodsmentioning

confidence: 99%

Effect of ethyl methanesulfonate mediated mutation for enhancing morpho-physio-biochemical and yield contributing traits of fragrant rice

Shamshad,

Rashid,

Jankuloski

et al. 2023

PeerJ

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Background Chemical mutagenesis has been successfully used for increasing genetic diversity in crop plants. More than 800 novel mutant types of rice (Oryza sativa L.) have been developed through the successful application of numerous mutagenic agents. Among a wide variety of chemical mutagens, ethyl-methane-sulfonate (EMS) is the alkylating agent that is most commonly employed in crop plants because it frequently induces nucleotide substitutions as detected in numerous genomes. Methods In this study, seeds of the widely consumed Basmati rice variety (Super Basmati, Oryza sativa L.) were treated with EMS at concentrations of 0.25%, 0.50%, 0.75%, 1.0%, and 1.25% to broaden its narrow genetic base. Results Sensitivity to a chemical mutagen such as ethyl methanesulfonate (EMS) was determined in the M1 generation. Results in M1 generation revealed that as the levels of applied EMS increased, there was a significant reduction in the germination percent, root length, shoot length, plant height, productive tillers, panicle length, sterile spikelet, total spikelet, and fertility percent as compared to the control under field conditions. All the aforementioned parameters decreased but there was an increase in EMS mutagens in an approximately linear fashion. Furthermore, there was no germination at 1.25% of EMS treatment for seed germination. A 50% germination was recorded between 0.50% and 0.75% EMS treatments. After germination, the subsequent parameters, viz. root length and shoot length had LD50 between 05.0% and 0.75% EMS dose levels. Significant variation was noticed in the photosynthetic and water related attributes of fragrant rice. The linear increase in the enzymatic attributes was noticed by the EMS mediated treatments. After the establishment of the plants in the M1 generation in the field, it was observed that LD50 for fertility percentage was at EMS 1.0% level, for the rice variety. Conclusion Hence, it is concluded that for creating genetic variability in the rice variety (Super Basmati), EMS doses from 0.5% to 0.75% are the most efficient, and effective.

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“…Biological damages induced by mutagen could be considered an indicator of mutagenic sensitivity of the genotypes [ 46 ]. In the present study, higher mutagen doses induced more reduction in bio-physiological traits that might be attributed to cellular disturbances [ 47 ]. The results were in accordance with the findings of [ 48 ] in Cicer arietinum and are discussed below:…”

Section: Discussionmentioning

confidence: 90%

Gamma rays and sodium azide induced variations in bio-physiological and agronomical traits in linseed (Linum usitatissimum L.)

Jahan,

Raina,

Malik

et al. 2024

Heliyon

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Mutagenic Effect on Seed Germination, Seedling Growth and Seedling Survival of Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp)

Cited by 6 publications

References 0 publications

Assessment on Seedling Traits of Black Gram (Vigna mungo L. Hepper) Mutants Raised during M2 Generation

Assessment on Seedling Traits of Black Gram (Vigna mungo L. Hepper) Mutants Raised during M2 Generation

Effect of ethyl methanesulfonate mediated mutation for enhancing morpho-physio-biochemical and yield contributing traits of fragrant rice

Gamma rays and sodium azide induced variations in bio-physiological and agronomical traits in linseed (Linum usitatissimum L.)

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