Mutagenic and genotoxic effects induced by PM0.5 of different Italian towns in human cells and bacteria: The MAPEC_LIFE study

Bonetta, Sara; Bonetta, Silvia; Schilirò, Tiziana; Ceretti, Elisabetta; Feretti, Donatella; Covolo, Loredana; Vannini, Samuele; Villarini, Milena; Moretti, Massimo; Verani, Marco; Carducci, Annalaura; Bagordo, Francesco; Donno, Antonella De; Bonizzoni, Silvia; Bonetti, A.; Pignata, Cristina; Carraro, Elisabetta; Gelatti, Umberto; Gilli, Giorgio; Romanazzi, Valeria; Gea, Marta; Festa, Andrea; Viola, Gaia Claudia Viviana; Zani, Claudia; Zerbini, Ilaria; Donato, Francesco; Monarca, Silvano; Fatigoni, Cristina; Levorato, Sara; Salvatori, Tania; Donzelli, Gabriele; Palomba, Giacomo; Casini, Beatrice; Giorgi, Maria Grazia De; Devoti, Gabriele; Grassi, Tiziana; Idolo, Adele; Panìco, Alessandra; Serio, Francesca; Furia, C.; Colombi, Paolo

doi:10.1016/j.envpol.2018.11.017

Cited by 29 publications

(22 citation statements)

References 61 publications

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“…Results obtained in the present study evidenced a possible genotoxic effect of fine PM2.5 on human lymphocytes, at all tested concentrations. Showed data seem to be concordant with results obtained by other authors although these last used different assays (chromosomal aberration test or Comet assay) and observed a significant increase of genotoxicity at generally higher concentrations of PM (for example 33, 100, 300 µg/mL) 14,41,42 . The mechanism by which PM induces genotoxicity, could be linked to the production of ROS.…”

Section: Discussionsupporting

confidence: 90%

In vitro genomic damage induced by urban fine particulate matter on human lymphocytes

Santovito

Gendusa

Cervella

et al. 2020

Sci Rep

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Urban air pollution represents a global problem, since everyday many mutagenic and carcinogens compounds are emitted into the atmosphere, with consequent adverse health effects on humans and biota. Specifically, particulate matter air pollution was associated with increased risks in human mortality and morbidity. In this paper, we analyse the genomic effects on human lymphocytes of different concentrations of annual Turin PM2.5 extract by an in vitro micronuclei assay. Samplings were collected from an urban meteorological-chemical station positioned in Turin (Italy), one of the most polluted cities in Europe. PM2.5 sampled on filters was used for organic extraction in monthly pools and successively aggregated to produce a mixture representative for a full year PM2.5 collection. Lymphocytes were exposed to four concentrations of PM2.5: 5, 10, 15 and 20 μg/mL and micronuclei, nucleoplasmic bridges and nuclear buds were scored. With respect to controls, PM2.5 significantly increased the frequencies of all analysed biomarkers at all tested concentrations, whereas the CBPI index was significantly reduced only at the concentration of 20 μg/mL. Such in vitro effects can both to stimulate local authorities to adopt efficient measures for air pollution mitigation and to improve human monitoring to detect early precancer lesions. Epidemiological studies suggested that particulate matter (PM) air pollution in the urban environment could be associated with an increase in several diseases, including cancer 1 and cardiopulmonary diseases, as well as with a general increased risk in human mortality and morbidity 2-4. Indeed, in 2016, WHO reported that ischaemic heart disease account about 36% of the deaths attributable to ambient air pollution globally 5. Furthermore, IARC classified the outdoor air pollution as carcinogenic to humans (Group 1), particulate matter was evaluated separately and was also classified as Group 1 6,7. PM2.5 levels, size and chemical composition varied in relation to the local main emissions and orographic conformation. The early effects of the PM2.5 human exposure are not clearly understood. It is well known that the PM adverse effects on human depends on its physical characteristics and chemical composition 8. At cellular level, PM can induce oxidative stress as result of cell homeostasis unbalance and subsequent mitochondrial damage 2,9,10 , whereas, at genomic level, an excessive production of reactive oxygen species (ROS) was found to be associated to DNA damage and expression, with consequent increased risk of apoptosis for the cell 11. PM10 and PM2.5 are two of the most discussed parameters into the air pollution evaluation 12,13. Such particles, especially the finest fraction, carried a wide range of mutagenic, genotoxic and carcinogenic compounds. In vitro tests are defined indispensable first-line tools to detect the global mixture DNA-damage effect 14. In particular, organic extracts of urban air particles were found to induce cancer in animals and mutagenic effect in bacteria, plant and mam...

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Section: Discussionsupporting

confidence: 90%

In vitro genomic damage induced by urban fine particulate matter on human lymphocytes

Santovito

Gendusa

Cervella

et al. 2020

Sci Rep

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“…Many studies have shown that urban fine particulate matter (PM) has mutagenic and genotoxic activities in different short-term tests on bacteria and in vitro and in vivo human cells [ 1 , 2 , 3 , 4 , 5 , 6 ]. The genotoxic and mutagenic activity of PM varies according to its size and physical and chemical composition that depends mainly on local emissions [ 3 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Genotoxic Activity of Particulate Matter and In Vivo Tests in Children Exposed to Air Pollution

Zani

Donato

Ceretti

et al. 2021

IJERPH

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The aim of this paper was to investigate the relationship between micronuclei and DNA damage in children’s buccal mucosa cells and the genotoxicity and mutagenicity of the different sized fractions of particulate matter as well as the concentration of PAHs (polycyclic aromatic hydrocarbons) and metals in particulate matter. Air particulate matter was collected by high volume samplers located near the schools attended by the children on the same days of biological samplings. The mutagenic activity was assessed in different cells in in vitro tests (Ames test on bacteria and comet test on leukocytes). Our study showed weak positive correlations between (a) the mutagenicity of the PM0.5 fraction and PAHs and (b) the micronuclei test of children’s buccal cells and PAHs detected in PM0.5 and PM0.5–3 fractions. A positive correlation was also found between in vitro comet test on leukocytes and PAHs in the PM3–10 fraction. No correlation was observed for metal concentrations in each PM fraction.

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“…Particulate matter (PM), and, in particular, PM 10 , is well known to have toxic and carcinogenic effects on the human population [12]. However, in the most recent studies investigating the frequency of MN in populations exposed to atmospheric pollution, the effects of fine particulates (PM 2.5 -PM 1 ), quasi ultrafine particulates (PM 0.5 -PM 0.3 ), and ultrafine particulates (PM 0.1 ) have been studied more extensively than PM 10 [13,14].…”

Section: Introductionmentioning

confidence: 99%

Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

Panìco

Grassi

Bagordo

et al. 2020

IJERPH

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Micronuclei (MN) are biomarkers of early biological effect often used for detecting DNA damage in human population exposed to genotoxic agents. The aim of this study was to evaluate the frequency of MN in exfoliated buccal cells of children living in an industrialized (impacted) area compared with that found in children living in a control area without significant anthropogenic impacts. A total of 462 6–8-year-old children (206 in the impacted area, 256 in the control area) attending primary school were enrolled. A questionnaire was administered to the parents of the recruited children to obtain information about personal data, lifestyles, and food habits of their children. Atmospheric particulate fractions were collected near the involved schools to assess the level of environmental exposure of the children. The presence of MN was highlighted in 68.4% of children living in the impacted area with a mean MN frequency of 0.66‰ ± 0.61‰. MN positivity and frequency were significantly lower in the control area (37.1% and 0.27‰ ± 0.43‰, respectively). The frequency of MN was positively associated with quasi-ultrafine particulate matter (PM0.5), traffic near the home, and consuming barbecued food; while adherence to the Mediterranean diet and practicing sport were negatively associated.

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Mutagenic and genotoxic effects induced by PM0.5 of different Italian towns in human cells and bacteria: The MAPEC_LIFE study

Cited by 29 publications

References 61 publications

In vitro genomic damage induced by urban fine particulate matter on human lymphocytes

In vitro genomic damage induced by urban fine particulate matter on human lymphocytes

Genotoxic Activity of Particulate Matter and In Vivo Tests in Children Exposed to Air Pollution

Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

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