Mutagenic and antimutagenic effects ofEugenia unifloraL. by theAllium cepaL. test

Abstract

Eugenia uniflora (Surinam cherry tree) is an important species in traditional medicine and is used to decrease blood pressure and prevent heartburn, bronchitis, colic and stomachaches. The tealeaves have antirheumatic, anti-dysenteric, and febrifuge properties and are used to treat diabetes. This study aimed to assess the antiproliferative, mutagenic and antimutagenic effects of the leaves and fruits of E. uniflora through the Allium cepa test. The infusions were prepared using dried leaves (6.l −1 and 24 gl −1 ) and the oil extraction was undertaken using hydrolyzation (0.25%, in ethanol). For juice preparation, fruit pulp with and without seeds was used. Allium cepa root tips were used for slide preparation following the squashing technique. Analysis of 500 cells for each bulb was undertaken, 250 of each root, summing 2500 cells per treatment, a total of 17,500 and 10,000 cells in the first and second experiments, respectively. The mitotic index was calculated and statistical analyses were performed. We found that the aqueous extract and oil of E. uniflora has mutagenic activity at low concentrations and the extract at a higher than average concentration can be considered antimutagenic. Furthermore, both juice extracts with and without seeds have anti-proliferative capacity.

Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE)

“…In another study, Kuhn et al (2015) evaluated the antimutagenic activity of aqueous extracts of Eugenia uniflora L. (Surinam cherry) and; in this study, the authors also observed that E. uniflora extracts at highest concentration (24 g/l) showed antimutagenic effect.…”

“…Pers., Mikania glomerata Spreng., Mikania cordifolia (L. f.) Willd. and Eugenia uniflora L., investigated by Tedesco (2009), Nora et al (2010), Dias et al (2014) and Kuhn et al (2015), respectively.…”

Toxic and genotoxic effects of aqueous extracts of Poligonum weyrichii Fr. Schmidt on the Allium test taken as an example.

“…To assess these changes is possible by a number of tests on different objects, such as rodents (Carver et al, 1983) In this study we used the Allium test because it is an express-test, and is effective and sensitive when used in biological monitoring. It allows us to assess the environmental pollution, toxicity of different compounds, particles, physical factors and plant extracts (Levan, 1938;Frescura et al, 2012;Frescura et al, 2013;Kuhn et al, 2015;Bolsunovsky et al, 2019;Bernardes et al, 2019). The Allium test allows us to assess the cyto-and genotoxicity of the factors of different nature, not spending a lot of physical and economical resources (Teixeira et al, 2003), to avoid solving the problems connected with ethical use of plant objects; it provides with large amounts of data and the results are correlated with those obtained on cell lines (Tedesco and Laughinghouse, 2012).…”

ANÁLISE PROLIFERATIVA E FITOQUÍMICA DE Commelina erecta L. PELO TESTE Allium cepa L.

“…KUHN et al (2015) e ROSSATO et al (2010, avaliando o efeito das infusões de Eugenia uniflora L e Pluchea sagittalis. concluíram que as mesmas possuem ação antiproliferativa sobre o sistema teste de A. cepa.…”