Mutagenic activity of glycine upon nitrosation in the presence of chloride and human gastric juice: A possible role in gastric carcinogenesis

Gaspar, Jorge; Laires, A.; Va, Shaginian; Pereira, Sónia; Mariano, Ana; Quina, M; Rueff, José

doi:10.1002/(sici)1520-6866(1996)16:5<275::aid-tcm4>3.0.co;2-f

Cited by 5 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the relevance of this situation in vivo in the human body remains to be established, since the concentration of nitrite is not sufficiently high [18] to allow coffee nitrosation to occur in all its potential extension. On the other hand, the results obtained after nitrosation of instant coffee and after nitrosation of both fractions obtained after the separation through XAD-2 resin showed Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Products derived from soya [5,6], broad beans [7], black beans [8], pickles [6], several fishes and smoked or grilled meats [9,10,11,12], tea [5], or wines [13,14] are examples of some food items with genotoxic activity after nitrosation. Secondary amines [15], amino acids [16,17,18], heterocyclic amines [19], biogenic amines like tyramine [6,14], and several phenolic compounds [14] were identified as some of the precursors responsible for the genotoxic activity of various foodstuffs after nitrosation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotoxicity of instant coffee and of some phenolic compounds present in coffee upon nitrosation

Duarte

Laires

Gaspar

et al. 2000

Teratog. Carcinog. Mutagen.

View full text Add to dashboard Cite

Instant coffee exhibits genotoxic activity upon nitrosation at acidic pH values in the Ames tester strain TA100. Using adsorption chromatography (Amberlit XAD-2) it was observed that the major fraction of molecules responsible for the genotoxic activity upon nitrosation was not retained on this resin, suggesting that the polar molecules present in instant coffee could be responsible for the genotoxicity observed upon nitrosation. Some phenolic molecules present in instant coffee (catechol, caffeic acid, and chlorogenic acid) were also genotoxic upon nitrosation under the same experimental conditions. The concentrations of nitrosatable phenolic compounds in the studied coffee were determined by HPLC and their contributions to the total genotoxicity observed were studied. The results obtained suggest that besides phenolic compounds other molecules were also involved in the genotoxicity of this beverage upon nitrosation. Teratogenesis Carcinog. Mutagen. 20:241-249, 2000.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genotoxicity of instant coffee and of some phenolic compounds present in coffee upon nitrosation

Duarte

Laires

Gaspar

et al. 2000

Teratog. Carcinog. Mutagen.

View full text Add to dashboard Cite

show abstract

Mutagenicity of bisphenol A (4,4′‐isopropylidenediphenol) in vitro: effects of nitrosylation

Schrader

Langlois

Soper

et al. 2002

Teratog Carcinog Mutagen

View full text Add to dashboard Cite

Bisphenol A (4,4'-isopropylidenediphenol) is a common component of polycarbonate plastics and epoxy resins. Since bisphenol A-containing plastics and resins have found uses in food-contact items, its potential migration into foodstuffs and possible health consequences have been the focus of many recent studies. However, the potential mutagenic activation of bisphenol A by nitrosylation has received little attention. Incubation of bisphenol A with sodium nitrite under acidic conditions produced a yellow-brown product. When nitrosylated bisphenol A was tested in the Ames Salmonella/microsome assay at 100 ng to 1 mg/plate, dose-dependent increases in mutagenicity were found in both TA98 and TA100 Salmonella strains. These results indicated the presence of a direct-acting mutagenic activity causing both frameshift and base pair mutations, respectively. When compared to colony formation in untreated controls, the addition of rat liver S9 for metabolic activation had little influence on revertant colony formation. Unreacted bisphenol A dissolved in DMSO, acidic buffer, or inactivated nitrosylation solution showed negligible mutagenicity. When the nature of the mutagenic changes was examined using the Ames II trade mark Assay, a variety of base pair changes was found including T:A to A:T - S9, G:C to A:T +/- S9,C:G to A:T +/- S9 and C:G to G:C +/- S9. Bisphenol A also induced frameshift mutations at G:C sites. In addition, the presence of electrophiles was shown by the production of an intensely coloured orange-red product upon incubation of nitrosylated bisphenol A with the nucleophile 4-(4'-nitrobenzyl)pyridine. These findings suggest that migration of bisphenol A into nitrite containing foodstuffs, or its ingestion in the presence of nitrite, could lead to the formation of mutagenic compounds.

show abstract

Stereochemical effects in the metabolic activation of nitrosopiperidines: correlations with genotoxicity

González‐Mancebo

Gaspar

Calle

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

View full text Add to dashboard Cite

Mutagenic activity of glycine upon nitrosation in the presence of chloride and human gastric juice: A possible role in gastric carcinogenesis

Cited by 5 publications

References 0 publications

Genotoxicity of instant coffee and of some phenolic compounds present in coffee upon nitrosation

Genotoxicity of instant coffee and of some phenolic compounds present in coffee upon nitrosation

Mutagenicity of bisphenol A (4,4′‐isopropylidenediphenol) in vitro: effects of nitrosylation

Stereochemical effects in the metabolic activation of nitrosopiperidines: correlations with genotoxicity

Contact Info

Product

Resources

About