Abstract:Site‐specific mutagenesis techniques, also known as site‐directed mutagenesis (SDM), aim to introduce precise alterations in any coding or noncoding
deoxyribonucleic acid (DNA)
sequence, usually
in vitro
. These modifications could be as small as a nucleotide or several hundreds; in one site or in multisite in the same DNA sequence. Recently, these alterations have been also developed
in vivo
. SDM success depends on how change… Show more
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