Mutagenesis of in vitro cultures of Miscanthus × giganteus cultivar Freedom and detecting polymorphisms of regenerated plants using ISSR markers

Perera, Dinum; Barnes, Daniel J.; Baldwin, Brian S.; Reichert, Nancy A.

doi:10.1016/j.indcrop.2014.12.005

Cited by 21 publications

(23 citation statements)

References 40 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of genetic divergence corroborate with the results obtained by Cheruvathur et al (2012), where the analysis of ISSR data in nine plants regenerated in vitro from R. nasutus callogenesis and the mother plant, the use of 12 ISSR markers indicated low genetic variability. In a study with ISSR markers in plants regenerated in vitro from the callogenesis, Perera et al (2015) concluded that these are useful in detecting polymorphisms in Miscanthus × Giganteus and can be used for generation and screening of mutants leading to the development of new cultivars.…”

Section: Molecular Analysismentioning

confidence: 99%

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

Machado¹,

Oliveira²,

Oliveira³

et al. 2018

JAS

View full text Add to dashboard Cite

Mangaba tree (Hancornia speciosa Gomes) is a tropical fruit species from Brazil and presents socioeconomic potential. The objective of this study was to determine antioxidant activity, rutin content and genetic similarity among in vivo and in vitro matrices and progenies of six accessions from the Mangaba Active Germplasm Bank of the Embrapa Coastal Tablelands, SE, Brazil. Young leaves of adult matrix plants and in vitro callus methanolic extracts obtained from young leaves resulted in differences between the accessions for the rutin content and high antioxidant activity. The Costa Azul accession, from Bahia, Brazil outstood with values above 3,000 g of dry extract/g of DPPH (2.2-diphenyl-1-picrilhydrazyl) in in vitro callus coming from nodal and internodal segments (3,023.73 and 3,136.80 g of dry extract/g of DPPH, respectively). Rutin was not found in extracts resulting from in vitro callus of mangaba tree. The difference among the values obtained for DPPH analysis are superior when compared with in vivo leaf extract, with the in vitro Costa Azul accession (nodal and internodal) outstanding, concluding that in vitro callus induction can interfere with chemical compounds of the plant. The markers detect genetic similarity under in vitro cultivation conditions. The Costa Azul accession group itself in isolation from others and EC 50 concentrations differentiated between leaf and callus are obtained. O EC 50 is superior in extracts deriving from in vitro callus, with the in vitro Costa Azul accession (nodal and internodal).

show abstract

Section: Molecular Analysismentioning

confidence: 99%

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

Machado¹,

Oliveira²,

Oliveira³

et al. 2018

JAS

View full text Add to dashboard Cite

show abstract

“…For abiotic stress treatments, it is more convenient to control the culture conditions than in the field environment [20,40]. Salt, drought, cold, and heavy metal tolerance have been successfully performed in many plants.…”

Section: Availability To Use Some Selective Agents In Culture Conditionsmentioning

confidence: 99%

“…Morphological and physiological changes should be used in combination to discriminate the mutants. All the parameters such as leaf injury, slower growth, average number of shoots per explant, survival percentage of the plants, fresh weight of the explants, leaf photosynthetic capacity, antioxidant defense system, and accumulation of osmolytes should be investigated in detail especially for stress tolerance studies [40,41].…”

Section: New Insights On Gamma Raysmentioning

confidence: 99%

See 1 more Smart Citation

Applications of Ionizing Radiation in Mutation Breeding

Çelik¹,

Atak²

2017

New Insights on Gamma Rays

View full text Add to dashboard Cite

As a predicted result of increasing population worldwide, improvements in the breeding strategies in agriculture are valued as mandatory. The natural resources are limited, and due to the natural disasters like sudden and severe abiotic stress factors, excessive floods, etc., the production capacities are changed per year. In contrast, the yield potential should be significantly increased to cope with this problem. Despite rich genetic diversity, manipulation of the cultivars through alternative techniques such as mutation breeding becomes important. Radiation is proven as an effective method as a unique method to increase the genetic variability of the species. Gamma radiation is the most preferred physical mutagen by plant breeders. Several mutant varieties have been successfully introduced into commercial production by this method. Combinational use of in vitro tissue culture and mutation breeding methods makes a significant contribution to improve new crops. Large populations and the target mutations can be easily screened and identified by new methods. Marker assisted selection and advanced techniques such as microarray, next generation sequencing methods to detect a specific mutant in a large population will help to the plant breeders to use ionizing radiation efficiently in breeding programs.

show abstract

“…Freedom and Illinois, originated from a single genotype via vegetative propagation. Therefore, indirect regeneration procedures might enable generation of additional desired mutations (Perera et al 2015) and ploidy manipulations (Yu et al 2009), leading to new cultivar development.…”

Section: Introductionmentioning

confidence: 99%

Direct and indirect in vitro regeneration of Miscanthus × giganteus cultivar Freedom: effects of explant type and medium on regeneration efficiency

Perera

Barnes

Baldwin

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

Self Cite

View full text Add to dashboard Cite

Miscanthus × giganteus (giant miscanthus; Mxg) is a seed-sterile, perennial bioenergy crop with the potential to produce liquid fuel from lignocellulosic biomass. A new cultivar, Freedom, is being commercially grown in the USA on increasing acreage. To determine this genotype's regeneration responses in tissue culture, three explant sources were screened on media proven successful for other genotypes. Four callus induction media contained 13.6-22.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) alone or with 0.44-4.4 μM 6-benzyladenine (BA). Callus induction percentages for all explants ranged from 93 to 97%. Media yielding the greatest percentages of explants producing regenerable calli for shoot apices (from in vitro and greenhouse plant sources) were media containing either 13.6 μM 2,4-D plus 0.44 μM BA or 22.6 μM 2,4-D plus 0.44 μM BA. After culture on a regeneration medium containing 22 μM BA plus 1.3 μM naphthaleneacetic acid (NAA), 3.59-3.74 regenerants were obtained per explant. Immature inflorescence explants (from field-maintained plants) gave up to 77% regenerable calli and 6.99 regenerants per explant. Direct regenerants (shoots) arose from immature inflorescence explants on a medium containing 9.0 μM 2,4-D. Intact plants could be generated within 16-18 wk after culture initiation. Extensive visual assessments, and molecular assessments via inter-simple sequence repeat (ISSR) PCR analysis using 21 different primers, did not reveal distinguishable somaclonal variation among regenerants or when compared to rhizome-propagated transplants under field conditions. We believe that this is the first extensive in vitro and ex vitro analysis on a commercially grown Mxg genotype.

show abstract

Mutagenesis of in vitro cultures of Miscanthus × giganteus cultivar Freedom and detecting polymorphisms of regenerated plants using ISSR markers

Cited by 21 publications

References 40 publications

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

Applications of Ionizing Radiation in Mutation Breeding

Direct and indirect in vitro regeneration of Miscanthus × giganteus cultivar Freedom: effects of explant type and medium on regeneration efficiency

Contact Info

Product

Resources

About