Mutagenesis: A coherent technique to develop biotic stress resistant plants

Bhoi, Anita; Yadu, Bhumika; Chandra, Jipsi; Keshavkant, S.

doi:10.1016/j.stress.2021.100053

Cited by 17 publications

(2 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the rice cultivar ‘Zhefu 802’ which was produced through gamma-ray induction was widely planted in China in the 1990s, due to its excellent properties (increased tolerance to rice blast 17 ). In addition, gamma-induced Saccharum officinarum mutants have been reported to show resistance towards the sugarcane mosaic virus, increased growth performance (improved plant height, number of green leaves per plant and higher tiller number), increased drought tolerance as well as producing higher yield and sugar content 18 , 19 .…”

Section: Discussionmentioning

confidence: 99%

Genetic and phenotype recovery of Ananas comosus var. MD2 in response to ionizing radiation

Raffi

Rosli

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Due to their sessile nature, plants are exposed to various environmental stressors such as exposure to high levels of harmful ultraviolet (UV), ionizing, and non-ionizing radiations. This exposure may result in various damages, ranging from DNA and chromosomal aberrations to phenotypic abnormalities. As an adaptation, plants have evolved efficient DNA repair mechanisms to detect and repair any damage caused by exposure to these harmful stressors to ensure their survival. In this study, the effects of gamma radiation (as a source of ionizing radiation) on clonal Ananas comosus var. MD2 was evaluated. The morphology and physiology of the clonal plantlets before and after exposure to gamma radiation were monitored at specific time intervals. The degree of genetic variation between the samples pre- and post-irradiation was also analyzed by using inter-simple sequence repeat (ISSR) markers. The resulting data revealed that the heights of the irradiated plantlets were significantly reduced (compared to control), but improved with the recovery period. Irradiated samples also exhibited relatively good photosynthetic efficiency that further improved as the plantlets recover. These observations were supported by the ISSR analysis, where the genetic dissimilarities between the irradiated samples and control were reduced by 0.1017, after 4 weeks of recovery. Overall, our findings suggested that the phenotype recovery of the clonal A. comosus var. MD2 plantlets was contributed by their ability to detect and repair the DNA lesions (as exemplified by the reduction in genetic dissimilarity after 4 weeks) and hence allow the plantlets to undergo phenotype reversion to normal plant stature.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic and phenotype recovery of Ananas comosus var. MD2 in response to ionizing radiation

Raffi

Rosli

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Mutagenesis is a powerful tool for creating new germplasm resources and to clarify the function of plant genes ( Holme et al., 2019 ; Bhoi et al., 2022 ). Various artificial mutagenic techniques, such as T-DNA insertion mutations and physical mutagens, have been widely used in many kinds of plants.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of mutations induced by carbon ion beam irradiation in cotton

Liu

Zhao²,

Geng³

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Carbon ion beam (CIB) irradiation is a powerful way to create mutations in animals, plants, and microbes. Research on the mutagenic effects and molecular mechanisms of radiation is an important and multidisciplinary issue. However, the effect of carbon ion radiation on cotton is uncertain. In this study, five different upland cotton varieties and five CIB doses were used to identify the suitable irradiation dose for cotton. Three mutagenized progeny cotton lines from the wild-type Ji172 were re-sequenced. The effect of half-lethal dose on mutation induction indicated that 200 Gy with LETmax of 226.9 KeV/μm was the most effective heavy-ion dose for upland cotton and a total of 2,959-4,049 single-base substitutions (SBSs) and 610-947 insertion-deletion polymorphisms (InDels) were identified among the three mutants by resequencing. The ratio of transition to transversion in the three mutants ranged from 2.16 to 2.24. Among transversion events, G:C>C:G was significantly less common than three other types of mutations (A:T>C:G, A:T>T:A, and G:C>T:A). The proportions of six types of mutations were very similar in each mutant. The distributions of identified SBSs and InDels were similar with unevenly distributed across the genome and chromosomes. Some chromosomes had significantly more SBSs than others, and there were “hotspot” mutation regions at the ends of chromosomes. Overall, our study revealed a profile of cotton mutations caused by CIB irradiation, and these data could provide valuable information for cotton mutation breeding.

show abstract

Targeted Gene Editing in Pome Fruit Genetics and Breeding: State-of-the-Art, Application Potential and Perspectives

Claessen,

Aert,

De Storme

2023

A Roadmap for Plant Genome Editing

View full text Add to dashboard Cite

Even though traditional breeding of perennial fruit trees such as apple and pear has resulted in high performing cultivars in the past, it is a very lengthy and costly process that is unable to keep up with the increasing demands for improved yield, resistance and fruit quality posed by the growing world population and the rapidly changing climate. In the last decade, significant research advances have been made that can revolutionize pome fruit breeding to meet current needs, including the sequencing of apple and pear genomes, the increased understanding of associations between gene(s) and traits of interest, and the advancement in genetic engineering tools. In particular the emergence of genome-editing tools such as the CRISPR/Cas9 technology can significantly improve the speed and accuracy of pome fruit breeding programs. This chapter reviews the progress, opportunities and challenges of genome editing tools in apple and pear, and discusses the genetic basis of several important breeding goals to find possible targets for new gene-editing applications.

show abstract

Mutagenesis: A coherent technique to develop biotic stress resistant plants

Cited by 17 publications

References 83 publications

Genetic and phenotype recovery of Ananas comosus var. MD2 in response to ionizing radiation

Genetic and phenotype recovery of Ananas comosus var. MD2 in response to ionizing radiation

Genome-wide analysis of mutations induced by carbon ion beam irradiation in cotton

Targeted Gene Editing in Pome Fruit Genetics and Breeding: State-of-the-Art, Application Potential and Perspectives

Contact Info

Product

Resources

About