Mutacytin-1, a New C-Type Lectin-Like Protein from the Venezuelan Cuaima (<i>Lachesis muta muta</i>Linnaeus, 1766) (Serpentes: Viperidae) Snake Venom Inducing Cardiotoxicity in Developing Zebrafish (<i>Danio rerio</i>) Embryos

Zanotty, Yurisbeth; Álvarez, María Inés Fernández; Perdomo, Lourdes; Sánchez, Elda E.; Girón, María E.; Jiménez, Juan Carlos; Suntravat, Montamas; Guerrero, Belsy; Ibarra, Carlos Figueroa; Montero, Yuyibeth; Medina, Rafael; Navarrete, Luis F.; Rodrı́guez-Acosta, Alexis

doi:10.1089/zeb.2019.1731

Cited by 11 publications

(7 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Utilization of the zebrafish model in toxicology research has increased in the latest years, converting it in one of the best advantageous and convenient systems for understanding parameters of toxins on the hemostatic and cardiovascular systems (14). Even though there is considerable evolutionary separation between zebrafish and humans, significant genetic and phenotypic preservation in both systems have permitted substantial progresses in our understanding of how natural toxins act on the different organs and tissues of the human body.…”

Section: Discussionmentioning

confidence: 99%

“…It is also important to note that to date; no other studies are known to use the zebrafish model to assess the toxicity and lethality of the Tityus discrepans scorpion venom. However, we had assayed this methodology, characterizing a cardiotoxic effect in zebrafish larvae, produced by a toxin (Mutacytin-1) present in the venom of the Lachesis muta muta snake (14) and Bothrops venezuelensis venom. This toxin has coagulant activity (verified with the formation of a fibrin meshwork in platelet-poor human plasma and direct observation of clot formation in the heart of the animal model) and induces compromise of the cardiac system (decrease in cardiac frequency and output) (44).…”

Section: Discussionmentioning

confidence: 99%

“…Zebrafish were bred and keep up in the Electronic Microscopy Section, Anatomical Institute ''José Izquierdo,'' Universidad Central de Venezuela (Caracas, Venezuela), by an adjusted method (14). The fishes were preserved in 20-L tanks, previously filled with newly prepared filtered tap water (FTW), at 28°C, pH 6.6 -7.0, with 12-h light/12-h dark cycle.…”

Section: Breeding Of Zebrafish (Danio Rerio) Larvaementioning

confidence: 99%

See 2 more Smart Citations

Clinical cardiac alterations and hemostatic toxicities caused by scorpion (Tityus discrepans) venom and its purified fractions on zebrafish (Danio rerio) larvae

et al. 2021

Self Cite

View full text Add to dashboard Cite

Envenomation by the Venezuelan scorpion Tityus discrepans is typified by local and systemic alterations. The current work investigated the in vivo hemostatic processes, cardiac dysfunction and tissue destruction triggered by Tityus discrepans purified toxins 1 (3 kDa) and 2 (5 kDa) fractions. These fractions were obtained by C-18-HPLC chromatography. The hemostatic and cardiovascular toxicities in zebrafish of both fractions was assessed by means of specific phenotypic expressions and larvae behavior at 5, 15, 30, 40 and 60 min post-venom-treatment. The Tityus discrepans venom fractions 1 and 2 produced disseminated intravascular coagulation (presence of thrombus) in the central vein of the larva, heart rate/rhythm alterations, and necrotic events in more than 90% of all the larvae under their action. The outcomes have established the potential hemostatic and cardiovascular toxicities by Tityus discrepans venom, alerting on the possibility of cardiovascular injuries and thromboembolism in humans after scorpion stings envenomation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Breeding Of Zebrafish (Danio Rerio) Larvaementioning

confidence: 99%

See 1 more Smart Citation

Clinical cardiac alterations and hemostatic toxicities caused by scorpion (Tityus discrepans) venom and its purified fractions on zebrafish (Danio rerio) larvae

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, the zebrafish model was used to isolate the cardiotoxic molecule from Lachesis muta venom. First, crude venom was fractionated using SEC; then, the active fractions were fractionated using AEX, and fraction 8 with the highest cardiotoxicity was further purified using an RP-HPLC C18 column, generating a pure protein "mutacytin-1" [62]. Moreover, isolated rat mesenteric arteries might also be used to evaluate the vasorelaxant effect of fractions.…”

Section: Bioassay-guided Fractionationmentioning

confidence: 99%

Separation and Analytical Techniques Used in Snake Venomics: A Review Article

et al. 2022

View full text Add to dashboard Cite

The deleterious consequences of snake envenomation are due to the extreme protein complexity of snake venoms. Therefore, the identification of their components is crucial for understanding the clinical manifestations of envenomation pathophysiology and for the development of effective antivenoms. In addition, snake venoms are considered as libraries of bioactive molecules that can be used to develop innovative drugs. Numerous separation and analytical techniques are combined to study snake venom composition including chromatographic techniques such as size exclusion and RP-HPLC and electrophoretic techniques. Herein, we present in detail these existing techniques and their applications in snake venom research. In the first part, we discuss the different possible technical combinations that could be used to isolate and purify SV proteins using what is known as bioassay-guided fractionation. In the second part, we describe four different proteomic strategies that could be applied for venomics studies to evaluate whole venom composition, including the mostly used technique: RP-HPLC. Eventually, we show that to date, there is no standard technique used for the separation of all snake venoms. Thus, different combinations might be developed, taking into consideration the main objective of the study, the available resources, and the properties of the target molecules to be isolated.

show abstract

“…But, on the other hand, these proteases represent substantial tools for coagulation and thrombosis treatments and research of the mechanism implicated in circulatory system injury. This effective dose must be related with low molecular mass metalloproteases such as α and β ( ~22 kDa LHTa and ~23 kDa LHTb), proteases with potent haemorrhagic activity found in the crude venom of Lmm [16,27]. In the assessment of the haemorrhagic lesions were very well perceived on the skin of treated mice.…”

Section: (Cms-5) Showed Intense Fibrinolytic Activitymentioning

confidence: 99%

The Bushmaster (Cuaima) snake (Lachesis muta muta) of the Neotropical forest: the description of several haemostatic and biological activities in its venom

et al. 2021

Self Cite

View full text Add to dashboard Cite

Cuaima (Lachesis muta muta)(Lmm) is responsible for particular envenomations that occur mainly in people who are exploring forest areas, in logging activities, mining exploitation (mainly gold and diamonds) or indigenous people who live in small communities and develop their vital activity in tropical and subtropical jungles of the Venezuelan Lmm territory. A fibrinolytic toxin has been purified from the venom of bushmaster cuaima by size exclusion chromatography Sephacryl 300 chromatography column. Fractions collected with high fibrinolytic and scarce haemorrhagic activities were further fractionated by a cationic exchange Carboxymetil-Sepharose (CMS) chromatography column. From CMS was obtained a molecular mass ~26 kDa single band by SDS-PAGE, called Yuyilysin, which had fibrinolytic activity that was completely lost after treatment with ethylene di-amine tetra acetic acid (EDTA). In conclusion, Yuyilysin showed a broader spectrum of activities, with moderate haemorrhagic activity, non-oedematogenic achievement, but strong fibrinolytic and coagulant actions.

show abstract

Mutacytin-1, a New C-Type Lectin-Like Protein from the Venezuelan Cuaima (Lachesis muta mutaLinnaeus, 1766) (Serpentes: Viperidae) Snake Venom Inducing Cardiotoxicity in Developing Zebrafish (Danio rerio) Embryos

Cited by 11 publications

References 41 publications

Clinical cardiac alterations and hemostatic toxicities caused by scorpion (Tityus discrepans) venom and its purified fractions on zebrafish (Danio rerio) larvae

Clinical cardiac alterations and hemostatic toxicities caused by scorpion (Tityus discrepans) venom and its purified fractions on zebrafish (Danio rerio) larvae

Separation and Analytical Techniques Used in Snake Venomics: A Review Article

The Bushmaster (Cuaima) snake (Lachesis muta muta) of the Neotropical forest: the description of several haemostatic and biological activities in its venom

Contact Info

Product

Resources

About