Mussel-inspired triblock functional protein coating with endothelial cell selectivity for endothelialization

Zheng, Weiwei; Liu, Min; Qi, Haishan; Wen, Chiyu; Zhang, Chen; Mi, Jiali; Zhang, Lei; Fan, Daidi

doi:10.1016/j.jcis.2020.04.116

Cited by 22 publications

(13 citation statements)

References 56 publications

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“…One of the ECM proteins is laminin which affects cell attachment and spreading [3,30]. The reason is that cells with high surface adhesion and spreading rate are more prone to forming advanced tissues [32][33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Regulatory effects of laminin derived peptide on microtissue formation for tissue engineered scaffold-free constructs

Çevik

Ordek

Karaman

2022

The European Research Journal

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Objectives: Vascularization is an important stage for tissues and organs. The vascular network is succeeded by the attachment, spreading, proliferation of endothelial cells, and the completion of endothelialization. Endothelization can be mediated by laminin-derived peptides on microtissues. It is known that laminin-derived Tyr-Ile-Gly-Ser-Arg (YIGSR) peptide contributes to endothelial microtissue formation by promoting increased adhesion and proliferation of endothelial cells. This study aims to determine the efficacy of the laminin-derived YIGSR peptide in Human Umbilicial Vein Endothelial Cell (HUVEC) scaffold free microtissues (SFMs). Methods: After solid phase synthesis of YIGSR, microtissues were formed as SFMs. SFMs were cultured with 0 mM (control group), 1.5 mM and 3 mM YIGSR peptide. Diameters and viability analysis of HUVEC SFMs were performed on the 1 st , 4 th and 7 th days. Results: The diameters of control SFMs group decreased day by day. Diameters of 3 mM YIGSR SFMs increased on the 1 st and 4 th days but significantly decreased on the 7 th day. On the other hand, 1.5 mM YIGSR had a tendency on tissue formation because of increased diameter. As a result of the viability, YIGSR peptide increased cell viability. Conclusions: It has been determined that 1.5 mM YIGSR is the optimum amount for enlargement and viability of HUVEC SFMs. The concentration has contributed to proliferation and viability of endothelial SFMs. Thus, 1.5 mM YIGSR has been found as the most promising peptide concentration for increasing vascularization.

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Section: Discussionmentioning

confidence: 99%

Regulatory effects of laminin derived peptide on microtissue formation for tissue engineered scaffold-free constructs

Çevik

Ordek

Karaman

2022

The European Research Journal

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“…The synthetic nonbiodegradable polymers, such as PTFE, PU, and PET, with high biostable and biocompatible properties, have been mainly utilized to prepare large-diameter catheters and vessels. In order to avoid unspecific adsorption of proteins and improve endothelialization, antifouling modification [47][48][49][50] and peptides/proteins conjugation [51][52][53] are commonly used to tailor the surface properties of synthetic vascular grafts.…”

Section: Synthetic Nonbiodegradable Materialsmentioning

confidence: 99%

Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

Zhao

Feng

2020

Adv Healthcare Materials

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Cardiovascular devices have been widely applied in the clinical treatment of cardiovascular diseases. However, poor hemocompatibility and slow endothelialization on their surface still exist. Numerous surface engineering strategies have mainly sought to modify the device surface through physical, chemical, and biological approaches to improve surface hemocompatibility and endothelialization. The alteration of physical characteristics and pattern topographies brings some hopeful outcomes and plays a notable role in this respect. The chemical and biological approaches can provide potential signs of success in the endothelialization of vascular device surfaces. They usually involve therapeutic drugs, specific peptides, adhesive proteins, antibodies, growth factors and nitric oxide (NO) donors. The gene engineering can enhance the proliferation, growth, and migration of vascular cells, thus boosting the endothelialization. In this review, the surface engineering strategies are highlighted and summarized to improve hemocompatibility and rapid endothelialization on the cardiovascular devices. The potential outlook is also briefly discussed to help guide endothelialization strategies and inspire further innovations. It is hoped that this review can assist with the surface engineering of cardiovascular devices and promote future advancements in this emerging research field.

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“…In this method, autologous vascular endothelial cells (ECs) are implanted on the luminal surface of biomaterial scaffolds, and a monolayer of ECs is formed before implantation [ 6 ]. Nevertheless, in small-diameter vascular graft applications, the proliferation of vascular ECs along the biomaterial scaffolds luminal surface is impaired, which leads to the failure of vascular graft patency in the early stage of implantation [ 7 ]. To promote the efficiency of endothelialization, a variety of biomaterials, such as extracellular matrix (ECM)—based proteins [ 8 – 10 ] and glycosaminoglycans (GAGs) [ 11 ], are widely used to prepare vascular scaffolds promoting the proliferation of vascular ECs [ 10 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model

Niu

et al. 2021

J Nanobiotechnol

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One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia. The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model. Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method. The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood. Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency. At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery. This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction. Graphical Abstract

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Mussel-inspired triblock functional protein coating with endothelial cell selectivity for endothelialization

Cited by 22 publications

References 56 publications

Regulatory effects of laminin derived peptide on microtissue formation for tissue engineered scaffold-free constructs

Regulatory effects of laminin derived peptide on microtissue formation for tissue engineered scaffold-free constructs

Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model

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