Mussel‐Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene‐Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment

Low, Wei Ching; Rujitanaroj, Pim-on; Lee, Dong-Keun; Kuang, Jinghao; Messersmith, Phillip B.; Chan, Jerry Kok Yen; Chew, Sing Yian

doi:10.1002/mabi.201500101

Cited by 33 publications

(48 citation statements)

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“…Unmodified polydopamine and DHI coatings have been used to support the proliferation and differentiation of human neuronal stem cells and murine embryonic stem cells, respectively. Modified polycatecholamine coatings have also been used for this task with l ‐DOPA coatings modified with siRNA, enhancing neuronal differentiation of human mesenchymal stem cell . Also, polydopamine coatings on decellularized vein matrix and titanium have been modified with cell‐adhesion peptides and bovine serum albumin with a peptide aptamer, respectively .…”

Section: Applications Of Catecholamine Polymersmentioning

confidence: 99%

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Barclay

Hegab

Clarke

et al. 2017

Adv Materials Inter

278

223

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Almost ten years ago, Lee et al. [13] formulated a universal adhesive coating based on observation of the strong attachment by mussels through their byssal threads to virtually all types of inorganic and organic surfaces. The amino acid compositions of the mussel foot proteins that generate the attachment are rich in catechol and amine groups. [13][14][15] These groups associate under marine conditions, forming strong covalent and noncovalent interactions with substrates. [13] This led to the idea that both catechol and amine groups were crucial in forming robust, wide spectrum adhesive nanolayers [16,17] and consequently dopamine was conceived as a powerful building block for spontaneous deposition of thin polymer films. The dopamine monomer is deposited onto unadulterated surfaces through self-assembly and oxidative cross-linking from mild pH aqueous solution in a rapid reaction. This results in a durable, nanoscale, conformal, and hydrophilic coating that can be readily modified to introduce specific surface chemistries for a particular application. [18][19][20][21][22] These bioinspired, polydopamine materials are chemically and structurally indistinguishable from eumelanins found in the human body, [23,24] providing excellent biocompatibility. Additionally, polydopamine has broad electromagnetic absorption and excellent photothermal energy conversion [25] as well as having ionic-electronic hybrid conductivity. [26] Along with the excellent coating performance, these properties provide a vast array of potential applications for polydopamine materials.In this article, we explain what is known about polydopamine and related catecholamine coatings; their formation, the adhesion mechanism, and their physicochemical properties and we also review the applications of these materials (Figure 1). Since 2011, there have been a number of reviews on this subject matter, including general reviews on the physicochemical properties of polydopamine coatings [11,20,[27][28][29] and their applications. [20,27,30] There are also a number of more specific reviews on the chemical synthesis and modulation of polycatecholamine coatings, [31] the biomedical applications of these coatings, [8,[32][33][34] their electronic properties, [26,35] the use of polydopamine to make films at fluid interfaces, [36] in hierarchically constructed particles, [33] and capsules, [30] exploitation of polycatecholamine coatings in membrane filtration, [37] polydopamine-derived carbon materials, [38] and the use of coordination chemistry in catechol-based materials. [39] Nonetheless, this area of research is growing so rapidly [25,27] that many recent Polydopamine and related polycatecholamines can be easily deposited onto almost any surface from mild, aqueous solution. This results in durable, nanoscale coatings that exhibit high biocompatibility and have useful chemical and electronic properties. Additionally, these materials can be readily chemically and physically modified, and consequently, they are used extensively for surface modification. This ...

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Section: Applications Of Catecholamine Polymersmentioning

confidence: 99%

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Barclay

Hegab

Clarke

et al. 2017

Adv Materials Inter

278

223

View full text Add to dashboard Cite

show abstract

“…Accordingly, random nanofibers have been recently reported to significantly improve rat MSC proliferation compared to aligned nanofibers [99]. The [96] or in combination with sonic Hedgehog [105], graphene oxide [106], bioceramics [107], growth factors [108], acellular pericardium [109], platelet-rich plasma [110], collagen [104], fibronectin [111], and mussel inspired DOPA-melanin coating for the adsorption of siRNA targeting RE-1 silencing transcription factor [112]. In all cases, depending on the desired application, nanostructural surfaces (whether scaffolds or membranes) have been reported to provide a suitable system to support adhesion and growth of the cell type of interest, and to influence progenitor population activity and function, holding promise for tissue regeneration applications.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Nanotechnology for mesenchymal stem cell therapies

Corradetti

Ferrari

2016

Journal of Controlled Release

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“…[8,29] Scaffold-mediated RNA interference has been carried out through the surface immobilization or direct bulk encapsulation of siRNA in hydrogels, [30] nanofibers, [11] and sponge-based constructs. [8,29] Scaffold-mediated RNA interference has been carried out through the surface immobilization or direct bulk encapsulation of siRNA in hydrogels, [30] nanofibers, [11] and sponge-based constructs.…”

Section: Wwwadvancedsciencecommentioning

confidence: 99%

“…[8,9] Such systems are constructed through surface immobilization or direct bulk incorporation of siRNA, with or without a transfection agent, in nanofibers. [8,9] Such systems are constructed through surface immobilization or direct bulk incorporation of siRNA, with or without a transfection agent, in nanofibers.…”

Section: Introductionmentioning

confidence: 99%

“…[10,11] Among these systems, nanoparticles, formed by the electrostatic self-assembly of siRNA and loaded in electrospun fibers, are potentially promising for the provision of advanced sustainable gene silencing, but complex techniques of electrospinning are usually also required. [8,13] Moreover, certain types of nanoparticle, such as polyethylenimine (PEI), polyamidoamine dendrimers, polylysine, and chitosan, were shown to suffer from low efficiency and/or toxicity issues when incorporated into scaffolds. [8,13] Moreover, certain types of nanoparticle, such as polyethylenimine (PEI), polyamidoamine dendrimers, polylysine, and chitosan, were shown to suffer from low efficiency and/or toxicity issues when incorporated into scaffolds.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gene Silencing via PDA/ERK2‐siRNA‐Mediated Electrospun Fibers for Peritendinous Antiadhesion

Liu

et al. 2018

Advanced Science

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Sustained delivery of small interfering RNA (siRNA) is a challenge in gene silencing for managing gene‐related disorders. Although nanoparticle‐mediated electrospun fibers enable sustainable gene silencing, low efficiency, loss of biological activity, toxicity issues, and complex electrospinning techniques are all bottlenecks of these systems. Preventing peritendinous adhesion is crucial for their successful use, which involves blocking cellular signaling via physical barriers. Here, a multifunctional, yet structurally simple, cationic 2,6‐pyridinedicarboxaldehyde‐polyethylenimine (PDA)‐mediated extracellular signal‐regulated kinase (ERK)2‐siRNA polymeric delivery system is reported, in the form of peritendinous antiadhesion electrospun poly‐l‐lactic acid/hyaluronan membranes (P/H), with the ability to perform sustained release of bioactive siRNA for long‐term prevention of adhesions and ERK2 silencing. After 4 days of culture, the cell area and proliferation rate of chicken embryonic fibroblasts on siRNA+PDA+P/H membrane are significantly less than those on P/H and siRNA+P/H membranes. The in vivo results of average optical density of collagen type III (Col III) and gene expression of ERK2 and its downstream SMAD3 in the siRNA+PDA+P/H group are less than those of P/H and siRNA+P/H groups. Consequently, siRNA+PDA+P/H electrospun membrane can protect the bioactivity of ERK2‐siRNA and release it in a sustained manner. Moreover, adhesion formation is inhibited by reducing fibroblast proliferation and Col III deposition, and downregulating ERK2 and its downstream SMAD3.

show abstract

Mussel‐Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene‐Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment

Cited by 33 publications

References 46 publications

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Nanotechnology for mesenchymal stem cell therapies

Gene Silencing via PDA/ERK2‐siRNA‐Mediated Electrospun Fibers for Peritendinous Antiadhesion

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