Mussel-inspired enzyme immobilization and dual real-time compensation algorithms for durable and accurate continuous glucose monitoring

Kim, Kwang Bok; Choi, Hyung Kook; Jung, Hyun Joo; Oh, Young-Sook; Cho, Chul-Ho; Min, Jin Hong; Yoon, Seoyoung; Kim, Jaepil; Cho, Seong Je; Joon, Hyung

doi:10.1016/j.bios.2019.111622

Cited by 38 publications

(23 citation statements)

References 37 publications

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“…By contrast, extraction with hollow MNs preserves ISF composition as far as pertinent precautions are fulfilled to avoid sample evaporation and/or external contamination, which is a common issue whatever the collection method. The main limitation of ISF collection with MNs is that the volume is very low, usually less than 10 μL. , Thus, in view of this problem, most in vivo studies using MN sensors attempt a validation by inducing changes in analyte concentration , or comparing with concentration in blood, which has been widely applied in the case of MNs for glucose detection. , Evidently, these strategies cannot be considered as pure analytical validation methods because they are either qualitative or do not consider the same fluid.…”

mentioning

confidence: 99%

Toward In Vivo Transdermal pH Sensing with a Validated Microneedle Membrane Electrode

et al. 2021

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We present herein the most complete characterization of microneedle (MN) potentiometric sensors for pH transdermal measurements for the time being. Initial in vitro assessment demonstrated suitable analytical performances (e.g., Nernstian slope, linear range of response from 8.5 to 5.0, and fast response time) in both buffer media and artificial interstitial fluid (ISF). Excellent repeatability and reproducibility together with adequate selectivity and resiliency facilitate the appropriateness of the new pH MN sensor for transdermal ISF analysis in healthcare. The ability to resist skin insertions was evaluated in several ex vivo setups using three different animal skins (i.e., chicken, pork, and rat). The developed pH MN sensor was able to withstand from 5 to 10 repetitive insertions in all the skins considered with a minimal change in the calibration graph (<3% variation in both slope and intercept after the insertions). Ex vivo pH measurements were validated by determining the pH with the MN sensor and a commercial pH electrode in chicken skin portions previously conditioned at several pH values, obtaining excellent results with an accuracy of <1% and a precision of <2% in all cases. Finally, pH MN sensors were applied for the very first time to transdermal measurements in rats together with two innovative validation procedures: (i) measuring subcutaneous pH directly with a commercial pH microelectrode and (ii) collecting ISF using hollow MNs and then the pH measurement of the sample with the pH microelectrode. The pH values obtained with pH MN sensors were statistically more similar to subcutaneous measurements, as inferred by a paired sample t -test at 95% of confidence level. Conveniently, the validation approaches could be translated to other analytes that are transdermally measured with MN sensors.

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mentioning

confidence: 99%

Toward In Vivo Transdermal pH Sensing with a Validated Microneedle Membrane Electrode

et al. 2021

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“…In order to demonstrate suprastructure as an enzymatic reaction platform, suprastructures are fabricated with a dispersion mixture of two different silica NPs encapsulating glucose oxidase (GOX) and horseradish peroxidase (HRP), which are useful enzymes for a variety of fields. [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ] GOX oxidizes glucose into gluconic acid and hydrogen peroxide, which has extensively been applied to develop the glucose‐, pH‐, and hydrogen peroxide‐responsive systems. HRP activates various dyes by consuming hydrogen peroxide, therefore, the combination of GOX and HRP have been used for glucose biosensors.…”

Section: Introductionmentioning

confidence: 99%

Multimodal Enzyme‐Carrying Suprastructures for Rapid and Sensitive Biocatalytic Cascade Reactions

Kim

Lee

et al. 2021

Advanced Science

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Colloidal assemblies of mesoporous suprastructures provide effective catalysis in an advantageousvolume-confined environment. However, typical fabrication methods of colloidal suprastructures are carried out under toxic or harmful conditions for unstable biomolecules, such as, biocatalytic enzymes. For this reason, biocatalytic enzymes have rarely been used with suprastructures, even though biocatalytic cascade reactions in confined environments are more efficient than in open conditions. Here, multimodal enzyme-and photocatalyst-carrying superstructures with efficient cascade reactions for colorimetric glucose detection are demonstrated. The suprastructures consisting of various functional nanoparticles, including enzyme-carrying nanoparticles, are fabricated by surface-templated evaporation driven suprastructure synthesis on polydimethylsiloxane-grafted surfaces at ambient conditions. For the fabrication of suprastructures, no additional chemicals and reactions are required, which allows maintaining the enzyme activities. The multimodal enzymes (glucose oxidase and peroxidase)-carrying suprastructures exhibit rapid and highly sensitive glucose detection via two enzyme cascade reactions in confined geometry. Moreover, the combination of enzymatic and photocatalytic cascade reactions of glucose oxidase to titanium dioxide nanoparticles is successfully realized for the same assay. These results show promising abilities of multiple colloidal mixtures carrying suprastructures for effective enzymatic reactions and open a new door for advanced biological reactions and enzyme-related works.

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“…The range of glucose in ISF is the closest to the range of blood glucose in both healthy and diabetic, which provides a theoretical basis for the development of an ISF glucose sensor. However, a large number of research works have shown that there is a time-lag phenomenon between the ISF glucose and blood glucose, that is, ISF reflects the change of blood glucose level with a certain delay, which ranges from about 4-10 min [13][14][15][16]. Transdermal biofluid extraction generally adopts reverse iontophoresis (RI) technology, which can achieve the purpose of rapid extraction of ISF.…”

Section: Non-invasive Blood Glucose Monitoringmentioning

confidence: 99%

“…Numerous technological innovations (including material, structural, and electrochemical analysis) have opened the door to a wide range of electrochemical glucose biosensors, making glucose sensors highly successful not only in invasive systems, but also in an increasing number of applications in non-invasive monitoring systems. In terms of enzymes, in addition to the common GOx-based [15,28,29], glucose sensors such as glucose dehydrogenase(GDH)-based [14,30], GOx/horseradish peroxidase (HRP) dual enzyme systems [31], β-hydroxybutyrate dehydrogenase (HBD) [11], and non-enzymes [32] have also been heavily researched and developed. The application of new materials, such as porous materials, nanomaterials, and electrocatalytic materials, plays a good role in enhancing the effect of immobilized enzymes, reducing the influence of external environment, and increasing the electron transfer rate, which further improve the sensor stability, sensitivity, and response time.…”

Section: Third-generation Glucose Biosensors-direct Gox Electrodementioning

confidence: 99%

Non-Invasive Blood Glucose Monitoring Technology: A Review

Liu

Chang

Chen

et al. 2020

Sensors

270

157

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In recent years, with the rise of global diabetes, a growing number of subjects are suffering from pain and infections caused by the invasive nature of mainstream commercial glucose meters. Non-invasive blood glucose monitoring technology has become an international research topic and a new method which could bring relief to a vast number of patients. This paper reviews the research progress and major challenges of non-invasive blood glucose detection technology in recent years, and divides it into three categories: optics, microwave and electrochemistry, based on the detection principle. The technology covers medical, materials, optics, electromagnetic wave, chemistry, biology, computational science and other related fields. The advantages and limitations of non-invasive and invasive technologies as well as electrochemistry and optics in non-invasives are compared horizontally in this paper. In addition, the current research achievements and limitations of non-invasive electrochemical glucose sensing systems in continuous monitoring, point-of-care and clinical settings are highlighted, so as to discuss the development tendency in future research. With the rapid development of wearable technology and transdermal biosensors, non-invasive blood glucose monitoring will become more efficient, affordable, robust, and more competitive on the market.

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Mussel-inspired enzyme immobilization and dual real-time compensation algorithms for durable and accurate continuous glucose monitoring

Cited by 38 publications

References 37 publications

Toward In Vivo Transdermal pH Sensing with a Validated Microneedle Membrane Electrode

Toward In Vivo Transdermal pH Sensing with a Validated Microneedle Membrane Electrode

Multimodal Enzyme‐Carrying Suprastructures for Rapid and Sensitive Biocatalytic Cascade Reactions

Non-Invasive Blood Glucose Monitoring Technology: A Review

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