Mussel-Inspired Dual-Cross-linking Hyaluronic Acid/ε-Polylysine Hydrogel with Self-Healing and Antibacterial Properties for Wound Healing

Abstract

Physicians have long been calling for an inherent antimicrobial wound dressing, which will be a great progress for treating complicated infections. Here, we report a novel bioadhesive hydrogel with inherent antibacterial properties prepared by mixing modified hyaluronic acid (HA) and ε-polylysine (EPL). This hydrogel can effectively kill Gram (+) and (−) bacteria for its high positive charge density on the surface. The sol−gel transition occurs within seconds via horseradish peroxidase enzymatic crosslinking and Schiff base reaction, which also allows the hydrogel to recover completely from destruction quickly within 5 min. In an infected rat wound model, histological studies indicated that the hydrogels effectively killed bacteria on the surface of wounds and accelerated wound healing. Histological analysis indicated that the thickness of the newborn skin, the density of the newborn microvascular, granulation tissue, and the collagen of rats treated with hydrogel dressings were twice as high as those treated by commercial fibrin glue. These results indicate that the HA/EPL hydrogel has great potential as an antibacterial wound dressing for future clinical applications.

An Artificial Phase‐Transitional Underwater Bioglue with Robust and Switchable Adhesion Performance

“…Many coacervates exhibit bulk adhesive behaviors, [30,31] but the influence of coacervation state on bulk adhesive strength has not been elucidated systematically.Inthis regard, the V40K72-SDBS wet adhesion under different temperatures was studied (Figure 3A;S upporting Information, Figure S10). At 25 8 8Cincoacervation state,the bulk adhesive strengths were 607 AE 47 kPa and 498 AE 75 kPa on PVC and glass substrates,respectively.Then at 4 8 8C, the coacervation of V40K72-SDBS complex disappeared and the underwater adhesion strength reduced to 56 AE 11 kPa and 80 AE 11 kPa, respectively.W ea lso did ac ycle regarding the adhesion experiments from 25 8 8Ct o48 8Ca nd then back to 25 8 8C.…”

Biomimetic Glycopolypeptide Hydrogels with Tunable Adhesion and Microporous Structure for Fast Hemostasis and Highly Efficient Wound Healing

“…The critical gelation concentration ( C cgc ) of a hydrogel was determined by a widely‐used inverted vial method, that is, at which concentration a hydrogel did not flow for 30 s when the vial inverted. [ 24 ] As for the preparation of R‐Gels, the non‐glycosylated P 80 D 20 (the subscript denotes the number of repeating units and/or grafting units) and the glycosylated P 60 G 20 D 20 and P 40 G 40 D 20 derived from PLL 100 instantly formed hydrogels at a C cgc of 12 wt%, that is, R‐Gels‐1, 2, 3, when mixed with a tiny of FeCl 3 (0.4 m m ). This suggests that multiple Fe 3+ ‐catechol coordination bonds formed to crosslink those catechol‐grafted polymer chains in PBS, and the glycosylation modification had no apparent effect on the gelation.…”

“…[ 20–22 ] Those biocompatible polymer hydrogels are of great interest in wound adhesives and dressings due to their high water contents, injectable/adaptable, and mechanical properties, wound adhesive and healing effects, and lower toxic risk. [ 14,23,24 ] To enhance wet tissue adhesion, various mussel‐inspired polymer hydrogels have been constructed by covalent, and/or non‐covalent bonds for wound adhesives and dressings, [ 16,25 ] which include natural polysaccharides such as chitosan, alginate, hyaluronic acid, and dextran, [ 9,14,26–28 ] microbial and/or synthetic polypeptides, [ 29–32 ] PEG derivatives, [ 5,15,33 ] and so on. In those cases, chitosan based hydrogels have blood‐clotting and antibacterial properties yet lower adhesion and relatively high hemolysis.…”

Membrane-disruptive peptides/peptidomimetics-based therapeutics: Promising systems to combat bacteria and cancer in the drug-resistant era

“…Thus, it is suitable to deliver cyapte (a PTA for biofilm EPS damage) and proline (procollagen component for improved cell proliferation) for synergistic biofilm eradication and subsequent healing cascade activation. Furthermore, hydrogels that used EPL or its derivative as the main matrix materials also exhibit high drug encapsulation and superior antimicrobial efficacy in promoting wound healing 277 , 278 , 279 .…”