Musculoskeletal tissue engineering: Adipose derived stromal cell implementation for the treatment of osteoarthritis

Tevlin, Ruth; Talbott, Heather E.; Huber, Julika; DiIorio, Sarah E.; Longaker, MT; Wan, Derrick C.

doi:10.1016/j.biomaterials.2022.121544

Cited by 20 publications

(14 citation statements)

References 152 publications

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“…This population, isolated and locally injected, might secrete numerous trophic and growth factors enhancing the regeneration of both bone (via BMP-2 production) and cartilage (involving direct engraftment and trophic factors), although biological mechanisms are not completely understood. 54,55 On the other hand, inflammatory processes might be effectively targeted by other medical procedures, including ozone therapy which has been progressively gaining interest in recent years. In more detail, oxygen-ozone (O 2 O 3 ) therapy has been recently applied in patients with MSDs with promising antinociceptive and anti-inflammatory effects.…”

Section: Biophysics and Medical Procedures In Musculoskeletal Disordersmentioning

confidence: 99%

Section: Biophysics and Medical Procedures In Musculoskeletal Disordersmentioning

confidence: 99%

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Myths and truths on biophysics-based approach in rehabilitation of musculoskeletal disorders

Sire

Lippi

Marotta

et al. 2023

Therapeutic Advances in Musculoskeletal

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Musculoskeletal disorders (MSD) are a crucial issue in current literature due to their impact on physical function, social, and economic costs. Rehabilitation plays a pivotal role in the therapeutic management of these disabling conditions with growing evidence underlining positive effects in improving functional outcomes. However, to date, several questions are still open about the mechanisms underpinning functional improvements while recent research is now focusing on a deeper understanding of the biophysical processes underpinning the macroscopical effects of these treatments. Thus, this narrative review aims at providing a comprehensive overview about the state of the art of biophysical dimensions of currently available treatments for MSD. PubMed, Scopus, CENTRAL, PEDro, and Web of Science were searched between March 2022 and October 2022 for in vitro and in vivo studies, clinical trials, systematic reviews, and meta-analysis addressing the issue of biophysics-based approach in rehabilitation of MSD. Our findings showed that a biophysical approach might be integrated into regenerative rehabilitation, aiming at enhancing regenerative processes by mechanical and biophysical stimuli. In addition, a biophysical-based approach has been proposed to improve knowledge about several instrumental physical therapies, including shock wave therapies, low-level laser therapy, ultrasound, short-wave diathermy, electrical stimulation, pulsed electromagnetic field, and vibration therapy. In accordance, emerging research is now focusing on the biophysical properties of several medical procedures to improve pain management in patients with MSD. Taken together, our results showed promising results of the integration of a biophysical-based approach in rehabilitation, albeit several limitations currently limit its implementation in routine clinical setting. Unfortunately, the state of the art is still inconclusive, and the low quality of clinical studies based on the biophysical approach did not provide clear treatment protocols. Further studies are needed to promote a precise rehabilitation approach targeting biological modification and enhancing the functional improvement of patients with MSDs.

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Section: Biophysics and Medical Procedures In Musculoskeletal Disordersmentioning

confidence: 99%

Section: Biophysics and Medical Procedures In Musculoskeletal Disordersmentioning

confidence: 99%

Myths and truths on biophysics-based approach in rehabilitation of musculoskeletal disorders

Sire

Lippi

Marotta

et al. 2023

Therapeutic Advances in Musculoskeletal

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“…(Kimiz-Gebologlu and Oncel, 2022;Paskeh et al, 2022;Yu et al, 2022) The potential of exosomes in tissue engineering has been valued as a promising strategy. (Alvarez-Erviti et al, 2011;Tevlin et al, 2022) For bone tissue engineering, the applications of exosomes are attracting significant attention. (Li et al, 2018) Various studies have demonstrated that stem cell derived exosomal-based nanomaterials could effectively restore the critical-sized bone defects and promote bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…(Li et al, 2018) Various studies have demonstrated that stem cell derived exosomal-based nanomaterials could effectively restore the critical-sized bone defects and promote bone regeneration. (Li et al, 2018;Tevlin et al, 2022) Exosomal-based therapeutic strategies for various bone defects are promising. Some studies have proven that exosomes played significant parts in various physiological or pathological progresses of bone tissues regeneration, such as immunomodulation, angiogenesis, and wound healing.…”

Section: Introductionmentioning

confidence: 99%

The biological applications of exosomal-based materials in bone/cartilage tissue engineering

Chen

Cheng²,

Zhang³

et al. 2023

Front. Mater.

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Exosomes are secreted by various cells including stem cells, dendritic cells, and tumor cells, also known as the cell-derived extracellular vesicles. Exosomes, can carry informative cargos from host cells, thus have been employed as potential nanomaterials for their multifarious biological functions in biomedical fields, such as drug and genes delivery, tumor targeting, and disease treatment. Recently, the biological applications of exosomes in bone tissue engineering have gained increasing attention. Some important progress has been made while the tissue regeneration and functional recovery of boneremain as the key challenges to be addressed. In this article, we first made a summary of exosomes and their applications in the regeneration of bone and cartilage tissue. Then, modification approaches used for exosomes to equip them with excellent capacities are summarized. Finally, current concerns and future outlooks of exosomes in bone/cartilage tissue engineering and regeneration are discussed.

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“…Wnt10b-overexpressed OP-ASCs dramatically improved the repair of the CSCD of ovariectomized osteoporotic mice in vivo.ASCs are favoured by a wide range of researchers because of their easily available and abundant content. Compared with BMSCs, ASCs are still easier to cultivate and preserve, grow faster, and are more resistant,42,43 which has made them a point of interest in recent research. In the study, we discovered that the OP-ASCs' osteogenic differentiation capabilities, as measured by Runx2 and Opn mRNA and protein expression, as well as the number of mineralized nodules generated, were much lower than that of ASCs from normal mice.The Wnt signalling pathway is regarded as an important pathway to regulate bone balance in osteoporotic mice 44,45.…”

mentioning

confidence: 99%

Wnt10b regulates osteogenesis of adipose‐derived stem cells through Wnt/β‐catenin signalling pathway in osteoporosis

Huang

Cai

et al. 2023

Cell Proliferation

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Our previous finding revealed that the Wnt10b RNA expression of osteoporotic adipose‐derived stem cells (OP‐ASCs) with impaired osteogenic capacity was significantly reduced than that of ASCs. There are no ideas that the relationship between the OP‐ASCs' impaired osteogenic potential and Wnt10b expression. This study aimed to indicate the potential molecular mechanisms and functional role of Wnt10b in OP‐ASCs, as well as to investigate a potential application to reverse the OP‐ASCs' impaired osteogenic differentiation potential. The OP‐ASCs and ASCs were harvested from the inguinal fat of osteoporosis (OP) mice with bilateral ovariectomy (OVX) and normal mice. qPCR and WB were used to detect the different levels of the expression of the Wnt10b RNA in both OP‐ASCs and ASCs. Lentiviral‐mediated regulation of Wnt10b expression was employed for OP‐ASCs, and the detection of the expression levels of key molecules in the Wnt signalling pathway and key osteogenic factors was performed through qPCR and WB in vitro experiments. The capacity of OP‐ASCs to osteogenesis was determined using alizarin red staining. Lastly, the repair effect of the BCP scaffolds incorporating modified OP‐ASCs on the critical‐sized calvarial defects (CSCDs) in OP mice was scanned and detected by micro‐computed tomography, haematoxylin and eosin staining, Masson's trichrome staining and immunohistochemistry. First, we discovered that both the RNA and protein expression levels of Wnt10b were significantly lower in OP‐ASCs than that in ASCs. In vitro experiments, upregulation of Wnt10b could activate the Wnt signalling pathway, and increase expression of β‐catenin, Lef1, Runx2 and osteopontin (Opn), thereby enhancing the osteogenic ability of OP‐ASCs. In addition, the OP‐ASCs with Wnt10b‐overexpressing could promote the repair of CSCD in osteoporotic mice with increasing new bone volume, bone mineral density, and increased expression of Opn in new bone in vivo. Taken together, overexpression of Wnt10b could partially facilitate the differentiation of OP‐ASCs towards osteogenesis and accelerated the healing of bone defects by activating the Wnt/β‐catenin signalling pathway in vitro and in vivo experiments. This study confirmed the important role of Wnt10b in regulating the osteogenic differentiation capability of OP‐ASCs and indicated Wnt10b could be a potential therapeutic target for reversing the impaired osteogenic capabilities of OP‐ASCs to therapy bone defects of OP patients.

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Musculoskeletal tissue engineering: Adipose derived stromal cell implementation for the treatment of osteoarthritis

Cited by 20 publications

References 152 publications

Myths and truths on biophysics-based approach in rehabilitation of musculoskeletal disorders

Myths and truths on biophysics-based approach in rehabilitation of musculoskeletal disorders

The biological applications of exosomal-based materials in bone/cartilage tissue engineering

Wnt10b regulates osteogenesis of adipose‐derived stem cells through Wnt/β‐catenin signalling pathway in osteoporosis

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