Muscular dystrophy in a dish: engineered human skeletal muscle mimetics for disease modeling and drug discovery

Smith, André; Davis, Jennifer; Lee, Gabsang; Mack, David L.; Kim, Deok Ho

doi:10.1016/j.drudis.2016.04.013

Cited by 48 publications

(42 citation statements)

References 114 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Through development of nanotopographical substrates, a novel disease model for Duchenne muscular dystrophy has been discovered [72, 73]. By using patient specific hiPSCs, this particular model can more accurately test disease phenotypes without the effort of in vivo testing.…”

Section: Biophysical Regulation Of Cell Reprogrammingmentioning

confidence: 99%

A biomaterial approach to cell reprogramming and differentiation

Long

Kim

et al. 2017

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

Cell reprogramming of somatic cells into pluripotent states and subsequent differentiation into certain phenotypes has helped progress regenerative medicine research and other medical applications. Recent research has used viral vectors to induce this reprogramming; however, limitations include low efficiency and safety concerns. In this review, we discuss how biomaterial methods offer potential avenues for either increasing viability and downstream applicability of viral methods, or providing a safer alternative. The use of non-viral delivery systems, such as electroporation, micro/nanoparticles, nucleic acids and the modulation of culture substrate topography and stiffness have generated valuable insights regarding cell reprogramming.

show abstract

Section: Biophysical Regulation Of Cell Reprogrammingmentioning

confidence: 99%

A biomaterial approach to cell reprogramming and differentiation

Long

Kim

et al. 2017

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

show abstract

“…Conversely, directed differentiation approaches have employed several methodologies, each aimed at recapitulating the early differentiation stages that take place in the embryo to generate skeletal muscle (Table S2). Reviews of the field have so far been mostly comparative, focusing on the different methods used to generate skeletal muscles in vitro and their relative success (Abujarour and Valamehr, 2015;Baker and Lyons, 1996;Smith et al, 2016). In the following sections, we will discuss these studies in the context of developmental and cell biology and consider the extent to which they recapitulate in vivo myogenesis.…”

Section: Skeletal Myogenesis In the Dish: Learning From Developmentmentioning

confidence: 99%

Making muscle: skeletal myogenesisin vivoandin vitro

2017

View full text Add to dashboard Cite

Skeletal muscle is the largest tissue in the body and loss of its function or its regenerative properties results in debilitating musculoskeletal disorders. Understanding the mechanisms that drive skeletal muscle formation will not only help to unravel the molecular basis of skeletal muscle diseases, but also provide a roadmap for recapitulating skeletal myogenesis in vitro from pluripotent stem cells (PSCs). PSCs have become an important tool for probing developmental questions, while differentiated cell types allow the development of novel therapeutic strategies. In this Review, we provide a comprehensive overview of skeletal myogenesis from the earliest premyogenic progenitor stage to terminally differentiated myofibers, and discuss how this knowledge has been applied to differentiate PSCs into muscle fibers and their progenitors in vitro.

show abstract

“…[96] Similar studies studying disease onset in other engineered tissues and organoids, such as skeletal muscle, brain, gut, kidney, and liver, have likewise contributed significantly to the scientific community's understanding of biological design principles. [97][98][99] …”

Section: Environmental Feedback and Adaptation In Engineered Biologicmentioning

confidence: 99%

Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence and Evolution of Biological Design

Raman

Bashir

2017

Adv Healthcare Materials

View full text Add to dashboard Cite

how the concept of bioinspiration, or imitating biological design and functionality in synthetic materials, created a new class of "smart" biomimetic materials. Then, we shall discuss how the continuing development of enabling manufacturing technologies, such as 3D printing and microfluidics, has established the separate subdiscipline of biofabrication for tissue engineering, or "building with biology." Finally, we shall investigate the convergence of these two fields into the emerging discipline of biohybrid design, the use of biological materials to power non-natural functional behaviors in synthetic machines. Throughout this report, we will revisit a single class of materials, hydrogels, as a case study of biological design in the context of each subfield, and discuss how the constraints, underlying principles, and end-use applications differ in each case. We will also discuss ethical considerations of biological design, with a special focus on forward engineering non-natural or hypernatural functional behaviors in biohybrid systems. We will conclude with recommendations for implementing biological design into educational curricula, ensuring effective and responsible practices for the next generation of engineers and scientists. Biomimicry and Bioinspired DesignA deeper understanding of the underlying design principles that govern biological systems has inspired the field of biomimicry. [1,2] Observing adaptive phenomena in nature, scientists and engineers have sought to extract the components of biological design responsible for this behavior and replicate the behavior in synthetic materials. Fundamentally, this involves understanding the building blocks or base units from which a biological material is built, the hierarchical assembly of these building blocks, and the interactions and interfaces between them.In this section, we will discuss strategies that engineers and scientists have employed to engineer bioinspired hierarchy, from bottom-up self-assembly and top-down engineered assembly. We will present several key demonstrations of stimulus-responsive hydrogels ranging from the micro-to the macroscale, and investigate novel demonstrations in biomimetic actuation and movement. We will conclude with the remaining challenges in the field of biomimicry and discuss the potential future impact of bioinspired design.The discipline of biological design has a relatively short history, but has undergone very rapid expansion and development over that time. This Progress Report outlines the evolution of this field from biomimicry to biofabrication to biohybrid systems' design, showcasing how each subfield incorporates bioinspired dynamic adaptation into engineered systems. Ethical implications of biological design are discussed, with an emphasis on establishing responsible practices for engineering non-natural or hypernatural functional behaviors in biohybrid systems. This report concludes with recommendations for implementing biological design into educational curricula, ensuring effective and responsible practice...

show abstract

Muscular dystrophy in a dish: engineered human skeletal muscle mimetics for disease modeling and drug discovery

Cited by 48 publications

References 114 publications

A biomaterial approach to cell reprogramming and differentiation

A biomaterial approach to cell reprogramming and differentiation

Making muscle: skeletal myogenesisin vivoandin vitro

Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence and Evolution of Biological Design

Contact Info

Product

Resources

About