Muscodor albus MOW12 an Endophyte of Piper nigrum L. (Piperaceae) Collected from North East India Produces Volatile Antimicrobials

Abstract

Muscodor albus MOW12, an endophytic fungus isolated from Piper nigrum in Mawlong, Meghalaya, India, resembles some cultural and hyphal characteristics of previous isolates of Muscodor sp. In addition, it possesses about 99 % similarity in its ITS rDNA with other M. albus isolates and thus is nicely centered within the genetic tree to other Muscodor spp. This xylariaceae fungus effectively inhibits and kills certain plant pathogenic fungi by virtue of a mixture of volatile compounds that it produces. The majority of these compounds were identified by gas chromatography/mass spectrometry as small molecular weight esters, alcohols, and acids. The main ester components of this isolate of M. albus in its volatile mixture are acetic acid, ethyl ester; propanoic acid, 2-methyl-, methyl ester and acetic acid, 2-methylpropyl ester. This appears to be the first report of any M. albus strain from India.

IAA Producing Enterobacter sp. I-3 as a Potent Bio-herbicide Candidate for Weed Control: A Special Reference with Lettuce Growth Inhibition

“…Plant growth inhibition of bacteria is caused by various mechanisms. Volatile materials arise from bacterial culture inhibit the plant growth and pathogens [6][7][8]. For instant, some species of Pseudomonas and Chromobacterium genus have a negative effect on plant growth due to the production of hydrogen cyanide.…”

The same host but a different Muscodor: A new Muscodor albus isolate from wild pineapple (Ananas ananassoides) with potential application in agriculture

“…These traits clearly distinguished the isolate aa3 from the reddish colonies of M. crispans (Mitchell et al, 2008), M. roseus (Worapong et al, 2002), M. oryzae and M. suthepensis (Suwannarach et al, 2013), and from the odd cauliflower-like structures present in M. crispans (Mitchell et al, 2008), M. cinnamomi (Suwannarach et al, 2010), M. tigerii (Saxena et al, 2015) and M. darjeelingensis ; and also the bud like structure described for M. strobelii . However, aa3 hyphae resembled those earlier depicted for M. albus (Banerjee et al, 2014(Banerjee et al, , 2010Ezra et al, 2004;Strobel et al, 2007Strobel et al, , 2001. Analyses of VOCs composition demonstrated that the isolate aa3 produces naphthalene and azulene derivatives (e.g.…”

“…In addition, the sesquiterpene with the higher RI (compound 33, M þ 222) and tentatively identified as bulnesol (Table 2), has never been reported as part of volatile mixture from M. albus isolates. It is also remarkable that VOCs produced by M. albus strain aa3 lacks esters, which are major compounds detected in the volatiles of all M. albus isolates (Banerjee et al, 2014(Banerjee et al, , 2010Ezra et al, 2004;Strobel et al, 2007Strobel et al, , 2001. Production of a volatile mixture with a divergent pattern from the consensus described for M. albus, has also been observed for the isolate GBA, originally collected from a Ginkgo biloba plant in USA (Banerjee et al, 2010).…”

“…Several strains of M. albus have been isolated from tropical trees and vine species around the world (Atmosukarto et al, 2005;Banerjee et al, 2014Banerjee et al, , 2010Ezra et al, 2004;Sopalun et al, 2003;Strobel et al, 2007Strobel et al, , 2001, indicating a wide distribution range of this species. In contrast, M. crispans has been merely isolated from an Ananas ananassoides (Baker) L.B.Sm (wild pineapple) plant found in the Bolivian Amazon (Mitchell et al, 2008).…”

Screening of Endophyte Piper betle Bacteria from the Forests of HPPB University Andalas as Antibiotics Producer

“…2). Exploration of betel endophytic microbes in the north-eastern part of the Indian state, found Muscodor albus which is a beta endophytic betel Piper nigrum that has strong antibiotic activity (Banerjee et al, 2014). Endophytic bacteria from betel P. colubrinum and P. nigrum are also antibiotic producers (Kulkarni et al, 2007).…”