Muscle temperature is least altered during total sleep deprivation in rats

Sharma, Bhavani Shankar; Sengupta, Trina; Vishwakarma, Lal Chandra; Akhtar, Nasreen; Mallick, Hruda Nanda

doi:10.1016/j.jtherbio.2021.102910

Cited by 3 publications

(4 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Values for static and peak dynamic firing rates and dynamic index shifted toward even higher values when the Bicarb‐buffer solution was warmed from 24°C to 34°C for a separate sample of classified afferents (Figure 4b ). At the warmer temperature, which is within 2.5°C of in vivo muscle temperature (Sharma et al., 2021 ), firing properties for subtype Ia extended values into the range established for the Ia afferents recorded from mice in vivo (cf. Carrasco et al., 2017 ).…”

Section: Resultsmentioning

confidence: 82%

Mechanosensory encoding in ex vivo muscle–nerve preparations

Housley

Gardolinski

Nardelli

et al. 2023

Experimental Physiology

View full text Add to dashboard Cite

New Findings What is the central question of this study? How well have studies using ex vivo muscle‐ nerve preparations represented in vivo features of sensory encoding by low threshold mechanoreceptors in muscle? What is the main finding and its importance? We find experimental adjustments to the ex vivo approach that improve representation of mechanosensory encoding observed in vivo. These adjustments will enhance accuracy in the search for molecular identity and encoding mechanisms of heterogeneous muscle mechanosensory neurons. AbstractOur objective was to evaluate an ex vivo muscle–nerve preparation used to study mechanosensory signalling by low threshold mechanosensory receptors (LTMRs). Specifically, we aimed to assess how well the ex vivo preparation represents in vivo firing behaviours of the three major LTMR subtypes of muscle primary sensory afferents, namely type Ia and II muscle spindle (MS) afferents and type Ib tendon organ afferents. Using published procedures for ex vivo study of LTMRs in mouse hindlimb muscles, we replicated earlier reports on afferent firing in response to conventional stretch paradigms applied to non‐contracting, that is passive, muscle. Relative to in vivo studies, stretch‐evoked firing for confirmed MS afferents in the ex vivo preparation was markedly reduced in firing rate and deficient in encoding dynamic features of muscle stretch. These deficiencies precluded conventional means of discriminating type Ia and II afferents. Muscle afferents, including confirmed Ib afferents were often indistinguishable based on their similar firing responses to the same physiologically relevant stretch paradigms. These observations raise uncertainty about conclusions drawn from earlier ex vivo studies that either attribute findings to specific afferent types or suggest an absence of treatment effects on dynamic firing. However, we found that replacing the recording solution with bicarbonate buffer resulted in afferent firing rates and profiles more like those seen in vivo. Improving representation of the distinctive sensory encoding properties in ex vivo muscle–nerve preparations will promote accuracy in assigning molecular markers and mechanisms to heterogeneous types of muscle mechanosensory neurons.

show abstract

Section: Resultsmentioning

confidence: 82%

Mechanosensory encoding in ex vivo muscle–nerve preparations

Housley

Gardolinski

Nardelli

et al. 2023

Experimental Physiology

View full text Add to dashboard Cite

show abstract

“…With the reduced consumption of energy by synaptic transmission during hyperpolarized down states, SWS represents an elective time for brain cells to carry out many housekeeping functions, including protein translation, the replenishment of calcium in presynaptic pools, the replenishment of glutamate vesicles, the recycling of membranes, the resting of mitochondria 2, 49–51 , and the metabolite clearance from the extracellular space 52 . Recently we have reported that the muscle temperature was least altered during the normal SW cycle in rats, indicating that probably the muscle atonia during REM sleep provides a conducive environment for the muscle to rest and repair 14 , somewhat similar to slow-wave activity during SWS for CNS synaptic remodeling.…”

Section: Discussionmentioning

confidence: 98%

“…However, as pointed by Matthew Walker in his book “Why We Sleep: The New Science of Sleep and Dreams” - there does not seem to be one major organ in the body or process within the brain that is not optimally enhanced by sleep and detrimentally impaired when we do not get enough sleep 11 . Previous reports suggest that sleep has a restorative effect at the systemic, cellular, and network-level of various organs other than the brain 12–14 . The most consistent daytime complaint associated with insomnia or sleep deprivation is fatigue 15–17 .…”

Section: Introductionmentioning

confidence: 99%

Acute sleep deprivation induces synaptic remodeling at the soleus muscle neuromuscular junction in rats

Sharma

Roy

Sengupta

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Sleep is important for cognitive and physical performance. Sleep deprivation not only affects neural functions but also results in muscular fatigue. A good night's sleep reverses these functional derangements caused by sleep deprivation. The role of sleep in brain function has been extensively studied. However, its role in neuromuscular junction or skeletal muscle morphology is sparsely addressed although skeletal muscle atonia and suspended thermoregulation during rapid eye movement sleep possibly provides a conducive environment for the muscle to rest and repair; somewhat similar to slow-wave sleep for synaptic downscaling. In the present study, we have investigated the effect of 24 h sleep deprivation on the neuromuscular junction morphology and neurochemistry using electron microscopy and immunohistochemistry in the rat soleus muscle. Acute sleep deprivation altered synaptic ultra-structure viz. mitochondria, synaptic vesicle, synaptic proteins, basal lamina, and junctional folds needed for neuromuscular transmission. Further acute sleep deprivation showed the depletion of the neurotransmitter acetylcholine and the overactivity of its degrading enzyme acetylcholine esterase at the neuromuscular junction. The impact of sleep deprivation on synaptic homeostasis in the brain has been extensively reported recently. The present evidence from our studies shows new information on the role of sleep on neuromuscular junction homeostasis and its functioning.

show abstract

“…Recently we have reported that the muscle temperature was least altered during the normal SW cycle in rats, indicating that probably the muscle atonia during REM sleep provides a conducive environment for the muscle to rest and repair 14 , somewhat similar to slow-wave activity during SWS for CNS synaptic remodeling.…”

Section: Acetylcholine and Acetylcholine Esterase Concentrationsmentioning

confidence: 98%

Acute sleep deprivation induces synaptic remodeling at the soleus muscle neuromuscular junction in rats

Sharma

Roy

Sengupta

et al. 2022

Sleep

Self Cite

View full text Add to dashboard Cite

Sleep is important for cognitive and physical performance. Sleep deprivation not only affects neural functions but also results in muscular fatigue. A good night's sleep reverses these functional derangements caused by sleep deprivation. The role of sleep in brain function has been extensively studied. However, its role in neuromuscular junction or skeletal muscle morphology is sparsely addressed although skeletal muscle atonia and suspended thermoregulation during rapid eye movement sleep possibly provide a conducive environment for the muscle to rest and repair; somewhat similar to slow-wave sleep for synaptic downscaling. In the present study, we have investigated the effect of 24 h sleep deprivation on the neuromuscular junction morphology and neurochemistry using electron microscopy and immunohistochemistry in the rat soleus muscle. Acute sleep deprivation altered synaptic ultra-structure viz. mitochondria, synaptic vesicle, synaptic proteins, basal lamina, and junctional folds needed for neuromuscular transmission. Further acute sleep deprivation showed the depletion of the neurotransmitter acetylcholine and the overactivity of its degrading enzyme acetylcholine esterase at the neuromuscular junction. The impact of sleep deprivation on synaptic homeostasis in the brain has been extensively reported recently. The present evidence from our studies shows new information on the role of sleep on neuromuscular junction homeostasis and its functioning.

show abstract

Muscle temperature is least altered during total sleep deprivation in rats

Cited by 3 publications

References 60 publications

Mechanosensory encoding in ex vivo muscle–nerve preparations

Mechanosensory encoding in ex vivo muscle–nerve preparations

Acute sleep deprivation induces synaptic remodeling at the soleus muscle neuromuscular junction in rats

Acute sleep deprivation induces synaptic remodeling at the soleus muscle neuromuscular junction in rats

Contact Info

Product

Resources

About