Muscle-specific actin expression in chondroblastomas

Povýšil, C; Tomanová, Radoslava; Matéjovský, Z

doi:10.1016/s0046-8177(97)90130-1

Cited by 30 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The basic cellular phenotype is further blurred by partly contradictory data on the expression of other cell lineage markers, such as epithelial cytokeratins, epithelial membrane antigen, and actin, which were not investigated in this study. 18,32,33 In our opinion, however, chondro-blastoma cells clearly express a mesenchymal phenotype, which is convincingly shown by the extensive expression and deposition of mesenchymal matrix gene products by the neoplastic cells.…”

Section: Discussionsupporting

confidence: 59%

Chondroblastoma is an osteoid-forming, but not cartilage-forming neoplasm

et al. 1999

View full text Add to dashboard Cite

Chondroblastoma is defined as a ‘benign tumour, characterized by highly cellular and relatively undifferentiated tissue composed of rounded or polygonal chondroblast‐like cells’ and the ‘presence of cartilaginous intercellular matrix’ (WHO). An extensive analysis of the extracellular matrix composition and gene expression pattern of a large series of chondroblastoma cases shows, however, that type II collagen, which is the main component of any cartilage matrix, is not expressed by the neoplastic cells of this tumour entity and is not deposited into the extracellular tumour matrix. Instead, osteoid and fibrous matrix is formed, with its typical biochemical composition. The multifocal expression of aggrecan proteoglycan in most chondroblastomas explains the bluish, pseudo‐chondroid appearance of some of the matrix‐rich areas of chondroblastomas. This study did not show chondroid matrix formation or chondroblastic cell differentiation in chondroblastomas, suggesting that chondroblastoma should be classified as a specific bone‐forming, rather than cartilage‐forming neoplasm. Copyright © 1999 John Wiley & Sons, Ltd.

show abstract

Section: Discussionsupporting

confidence: 59%

Chondroblastoma is an osteoid-forming, but not cartilage-forming neoplasm

et al. 1999

View full text Add to dashboard Cite

show abstract

“…A recent immunohistochemical study (36) revealed the presence of a-smooth muscle actin in some S-100-positive chondroblasts in three of 20 chondrosarcomas but in no chondrocytes contained in islands of chondroid matrix in these specimens. Other recent work demonstrated the presence of a-smooth muscle actin in cells of chondrocytic appearance (of spherical morphology and contained in lacunae) in the canine meniscus (32) and intervertebral disc (40) in vivo.…”

Section: And Intervertebral Disc (40) the Percentage Of Cells Contaimentioning

confidence: 81%

Distribution of chondrocytes containing α‐smooth muscle actin in human articular cartilage

Kim

Spector

2000

Journal Orthopaedic Research

View full text Add to dashboard Cite

Summary:Relatively littlc is known about the contractile behavior of the human articular chondrocyte. Other connective tissue cells are known to express a contractile actin isoform, a-smooth muscle actin, in response to injury, at selected stages of wound healing, and in certain pathological conditions. This and recent work demonstrating contractile behavior in adult canine articular chondrocytes in vitro prompted the present study of the distribution of a-smooth muscle actin-containing chondrocytes in human articular cartilage. Approximately 75% of the chondrocytes in the superficial region of cartilage expressed a-smooth muscle actin as demonstrated by immunohistochemistry. In contrast. only approximately 10% of the cells in the deep region stained for this contractile actin isoform. There was no correlation of the percentage of a-smooth muscle actin-positive cells in either region with Mankin grade or with age. This is the first report of a contractile potential for human articular chondrocytes. The roles of a-smooth muscle actin in these cells warrant further investigation. The question of whether it is necessary to refer to these cells as myochondrocytes is considered.Many phenotypic traits have been reported for the articular chondrocyte in health, disease, response to injury, and selected stages of healing (8,9,45). Chondrocyte behavior in vivo has been assessed dircctly by microscopy methods (31) and biochemical and molecular biological analysis of extracted cells (39) and indirectly by determining the biochemical makeup (44) and physical properties (21) of the matrix. Evaluation of the activities of isolated chondrocytes grown in v i m (10,20,29) has also added to our understanding of the profiles of genes expressed by this cell type under a range of environmental conditions.One of the features of the articular chondrocyte that has not yet been investigated is the specific actin composition of the cytoskeleton. Of the six actin isoforms-coded by different genes (22)-all cells contain the and one of the y iorms. In some connective tissue cells (dermal fibroblasts), alterations in chemical and physical environmental conditions can upregulate or down-regulate the expression of the gene for a contractile actin isoform, u-smooth muscle actin (13,18,42). Expression of this gene has been related to the contractile behavior of cells, which may

show abstract

“…In both entities, actin positivity is interpreted as signifying myofibroblastic differentiation (7, 8), which, in the chondromyxoid fibromas, is driven by the TGF-β1 pathway (8). The focal and weak reaction for osteonectin also occurred primarily in myofibroblast-like cells.…”

Section: Discussionmentioning

confidence: 99%

“…The other was finding marker(s) of future therapeutic value. We included D2-40 (podoplanin), a known marker of chondrosarcoma (2), bcl-2, an inhibitor of apoptosis and effector of the Indian hedgehog/parathyroid hormone-like hormone pathway, and COX-2, a mediator of angiogenesis, both molecular markers of possible therapeutic targeting [for a review, see (3)], YKL-40, a ‘high activity’ marker (4, 5), osteonectin (SPARC or BM-40), a known marker of osteosarcomas, chondrocytes and other mesenchymal cells (6), the muscle/myofibroblastic markers actin (7) and calponin (8), mdm-2, a marker of liposarcomas (9) and CD117 (c-kit), a marker of gastrointestinal stromal tumors with mutations in c-kit and PDGFRα (10). …”

mentioning

confidence: 99%

Markers aiding the diagnosis of chondroid tumors: an immunohistochemical study including osteonectin, bcl‐2, cox‐2, actin, calponin, D2‐40 (podoplanin), mdm‐2, CD117 (c‐kit), and YKL‐40

Daugaard

Christensen

Høgdall

2009

APMIS

View full text Add to dashboard Cite

Chondroid tumors comprise a heterogenous group of benign to overt malignant neoplasms, which may be difficult to differentiate from one another by histological examination. A group of 43 such tumors was stained with nine relevant antibodies in an attempt to find consistent marker profile(s) for the different subgroups. Archival material from three extraskeletal myxoid chondrosarcomas, five chordomas, five chondromyxoid fibromas, five chondroblastomas and 25 chondrosarcomas was stained with antibodies against osteonectin, bcl-2, cox-2, actin, calponin, D2-40 (podoplanin), mdm-2, CD117 (c-kit) and YKL-40. All 25 chondrosarcomas showed a positive staining reaction for D2-40, none for actin and CD117, and a partial reactivity for bcl-2 (36%). Chondroblastomas (5/5) and chondromyxoid fibromas (2/5) were the only tumors with a positive reaction for actin, and all chondroblastomas (n=5) and extraskeletal myxoid chondrosarcomas (n=3) were positive for bcl-2. In contrast to all other tumors, two of three extraskeletal myxoid chondrosarcomas were also positive for CD17 and negative for osteonectin, cox-2, mdm-2 and actin. All five chordomas were negative for D2-40 and positive for mdm-2 and YKL-40. The diagnosis of chondrosarcoma may be aided by its positivity for D2-40 and YKL-40 and its lack of reactivity for actin and CD117. This should be seen in the light of no reaction for D2-40 in chordomas and a corresponding lack of reaction for osteonectin, cox-2, mdm-2 and actin in extraskeletal myxoid chondrosarcomas. A convincing immunoreactivity for calponin and/or actin in chondromyxoid fibromas and chondroblastomas may also be helpful in differentiating these tumors from chondrosarcomas.

show abstract

Muscle-specific actin expression in chondroblastomas

Cited by 30 publications

References 26 publications

Chondroblastoma is an osteoid-forming, but not cartilage-forming neoplasm

Chondroblastoma is an osteoid-forming, but not cartilage-forming neoplasm

Distribution of chondrocytes containing α‐smooth muscle actin in human articular cartilage

Markers aiding the diagnosis of chondroid tumors: an immunohistochemical study including osteonectin, bcl‐2, cox‐2, actin, calponin, D2‐40 (podoplanin), mdm‐2, CD117 (c‐kit), and YKL‐40

Contact Info

Product

Resources

About