Muscle pain induced by static contraction in rats is modulated by peripheral inflammatory mechanisms

Santos, Diogo Francisco da Silva dos; Aquino, Bruna de Melo; Jorge, Carolina Ocanha; Azambuja, Graciana de; Schiavuzzo, Jalile Garcia; Krimon, Suzy; Neves, Juliana dos Santos; Parada, Carlos Amílcar; Oliveira, Maria Cláudia G.

doi:10.1016/j.neuroscience.2017.06.041

Cited by 14 publications

(16 citation statements)

References 64 publications

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“…In the present study, we used an animal model of musculoskeletal pain induced by static contraction, which is very similar to musculoskeletal pain frequently related to daily and work activities [11]. Specifically, work-related neck and shoulder pain is associated with static contraction of the trapezius muscle [9,10,[50][51][52].…”

Section: Discussionmentioning

confidence: 99%

“…The muscle hyperalgesia induced by static contraction was performed as described [11]. Initially, the rats were anesthetized with isoflurane and the static contraction was produced by applying electrical pulses through two electrodes (27 gauge) inserted into the belly of the gastrocnemius muscle.…”

Section: Muscle Hyperalgesia Induced By Static Contractionmentioning

confidence: 99%

“…Initially, the baseline muscle withdrawal threshold was quantified by three tests performed at 5-min intervals before static contraction. The mechanical muscle hyperalgesia was quantified as the change in mechanical nociceptive threshold calculated by subtracting the mean of measurements taken 1 h after the end of contraction [11]. Increases in hyperalgesia were represented by increase in the y-axis.…”

Section: Mechanical Muscle Nociceptive Threshold Testmentioning

confidence: 99%

“…To verify whether the static contraction-induced neutrophil migration [11] was modulated by P2X3 receptors, A-317491 was administered previously to static contraction (Fig. 4a).…”

Section: Static Contraction-induced Increase In Mpo Activity Is Modulmentioning

confidence: 99%

“…Specifically, the static contraction is the type of muscle contraction most related to daily and work activities [4][5][6][7] and has been associated with development of muscle pain [8][9][10]. Recently, we developed a new model of muscle pain induced by static contraction and showed that this muscle pain is modulated by inflammatory mediators, such as bradykinin, prostaglandins, sympathetic amines, and neutrophils in rats [11].…”

Section: Introductionmentioning

confidence: 99%

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P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration

Aquino

Santos

Jorge

et al. 2019

Purinergic Signalling

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P2X3 receptors are involved with several pain conditions. Muscle pain induced by static contraction has an important socioeconomic impact. Here, we evaluated the involvement of P2X3 receptors on mechanical muscle hyperalgesia and neutrophil migration induced by static contraction in rats. Also, we evaluated whether static contraction would be able to increase muscle levels of TNF-α and IL-1β. Male Wistar rats were pretreated with the selective P2X3 receptor antagonist, A-317491, by intramuscular or intrathecal injection and the static contraction-induced mechanical muscle hyperalgesia was evaluated using the Randall-Selitto test. Neutrophil migration was evaluated by measurement of myeloperoxidase (MPO) kinetic-colorimetric assay and the cytokines TNF-α and IL-1β by enzyme-linked immunosorbent assay. Intramuscular or intrathecal pretreatment with A-317491 prevented static contraction-induced mechanical muscle hyperalgesia. In addition, A-317491 reduced static contraction-induced mechanical muscle hyperalgesia when administered 30 and 60 min of the beginning of static contraction, but not after 30 and 60 min of the end of static contraction. Intramuscular A-317491 also prevented static contraction-induced neutrophil migration. In a period of 24 h, static contraction did not increase muscle levels of TNF-α and IL-1β. These findings demonstrated that mechanical muscle hyperalgesia and neutrophil migration induced by static contraction are modulated by P2X3 receptors expressed on the gastrocnemius muscle and spinal cord dorsal horn. Also, we suggest that P2X3 receptors are important to the development but not to maintenance of muscle hyperalgesia. Therefore, P2X3 receptors can be pointed out as a target to musculoskeletal pain conditions induced by daily or work-related activities.

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Section: Discussionmentioning

confidence: 99%

Section: Muscle Hyperalgesia Induced By Static Contractionmentioning

confidence: 99%

Section: Mechanical Muscle Nociceptive Threshold Testmentioning

confidence: 99%

“…To verify whether the static contraction-induced neutrophil migration [11] was modulated by P2X3 receptors, A-317491 was administered previously to static contraction (Fig. 4a).…”

Section: Static Contraction-induced Increase In Mpo Activity Is Modulmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration

Aquino

Santos

Jorge

et al. 2019

Purinergic Signalling

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Topical application of a TRPA1 antagonist reduced nociception and inflammation in a model of traumatic muscle injury in rats

Kudsi,

de David Antoniazzi,

Camponogara

et al. 2023

Inflammopharmacol

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P2X3 receptors contribute to transition from acute to chronic muscle pain

Jorge

Azambuja

Gomes

et al. 2020

Purinergic Signalling

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This study aimed to evaluate whether the development and/or maintenance of chronic-latent muscle hyperalgesia is modulated by P2X3 receptors. We also evaluate the expression of P2X3 receptors and PKCε of dorsal root ganglions during these processes. A mouse model of chronic-latent muscle hyperalgesia, induced by carrageenan and evidenced by PGE 2 , was used. Mechanical muscle hyperalgesia was measured by Randall-Selitto analgesimeter. The involvement of P2X3 receptors was analyzed by using the selective P2X3 receptors antagonist A-317491 by intramuscular or intrathecal injections. Expression of P2X3 and PKCε in dorsal root ganglion (L4-S1) were evaluated by Western blotting. Intrathecal blockade of P2X3 receptors previously to carrageenan prevented the development and maintenance of acute and chronic-latent muscle hyperalgesia, while intramuscular blockade of P2X3 receptors previously to carrageenan only reduced the acute muscle hyperalgesia and had no effect on chronic-latent muscle hyperalgesia. Intrathecal, but not intramuscular, blockade of P2X3 receptors immediately before PGE 2 , in animals previously sensitized by carrageenan, reversed the chronic-latent muscle hyperalgesia. There was an increase in total and phosphorylated PKCε 48 h after the beginning of acute muscle hyperalgesia, and in P2X3 receptors at the period of chronic muscle hyperalgesia. P2X3 receptors expressed on spinal cord dorsal horn contribute to transition from acute to chronic muscle pain. We also suggest an interaction of PKCε and P2X3 receptors in this process. Therefore, we point out P2X3 receptors of the spinal cord dorsal horn as a pharmacological target to prevent the development or reverse the chronic muscle pain conditions.

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Muscle pain induced by static contraction in rats is modulated by peripheral inflammatory mechanisms

Cited by 14 publications

References 64 publications

P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration

P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration

Topical application of a TRPA1 antagonist reduced nociception and inflammation in a model of traumatic muscle injury in rats

P2X3 receptors contribute to transition from acute to chronic muscle pain

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