Muscle fragments on a scaffold in rats: a potential regenerative strategy in urogynecology

Jangö, Hanna; Gräs, Søren; Christensen, Lise; Lose, Gunnar

doi:10.1007/s00192-015-2782-x

Cited by 9 publications

(8 citation statements)

References 22 publications

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“…After 8 weeks, there was a massive ingrowth of cells between and around the PCL fibers resulting in the formation of a neo-tissue/PCL construct, which was sufficiently strong to prevent hernia formation in a partial and even in a full thickness abdominal wall defect. In contrast to our previous findings with an MPEG-PLGA scaffold, 4–6 the present study demonstrated that the PCL scaffold seemed to be unable to function as a carrier or anchor for muscle stem cells in the form of autologous MFFs. No fluorescence-labelled or desmin-positive cells or fragments were recovered in association with the neo-tissue/PCL construct, and biomechanical testing showed no differences in parameters between scaffolds with or without MFFs.…”

Section: Discussioncontrasting

confidence: 99%

“…Load was plotted against elongation resulting in bilinear curves with low- and high-stiffness zones. 4 To calculate stress (MPa), the initial thickness and width of the specimens were measured without squeezing the tissue. Stress was plotted against strain (relative elongation), which also resulted in bilinear curves.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously implanted a methoxypolyethyleneglycol–polylactic-co-glycolic acid (MPEG–PLGA) scaffold, which is fully degraded within 8 weeks, and seeded it with fresh autologous muscle fiber fragments (MFFs) in various rat abdominal wall models. 4–6 MFFs have a noticeable ability to regenerate skeletal muscle tissue 7–9 thanks to their content of muscle stem cells, also called satellite cells, 10 which are capable of differentiating into new muscle fibers. In these studies, we found that cells from the MFFs survived and participated in the regenerative process, significantly affecting some of the biomechanical properties.…”

Section: Introductionmentioning

confidence: 99%

“…In these studies, we found that cells from the MFFs survived and participated in the regenerative process, significantly affecting some of the biomechanical properties. 4–6…”

Section: Introductionmentioning

confidence: 99%

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Examinations of a new long-term degradable electrospun polycaprolactone scaffold in three rat abdominal wall models

et al. 2017

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Alternative approaches to reinforce native tissue in reconstructive surgery for pelvic organ prolapse are warranted. Tissue engineering combines the use of a scaffold with the regenerative potential of stem cells and is a promising new concept in urogynecology. Our objective was to evaluate whether a newly developed long-term degradable polycaprolactone scaffold could provide biomechanical reinforcement and function as a scaffold for autologous muscle fiber fragments. We performed a study with three different rat abdominal wall models where the scaffold with or without muscle fiber fragments was placed (1) subcutaneously (minimal load), (2) in a partial defect (partial load), and (3) in a full-thickness defect (heavy load). After 8 weeks, no animals had developed hernia, and the scaffold provided biomechanical reinforcement, even in the models where it was subjected to heavy load. The scaffold was not yet degraded but showed increased thickness in all groups. Histologically, we found a massive foreign body response with numerous large giant cells intermingled with the fibers of the scaffold. Cells from added muscle fiber fragments could not be traced by PKH26 fluorescence or desmin staining. Taken together, the long-term degradable polycaprolactone scaffold provided biomechanical reinforcement by inducing a marked foreign-body response and attracting numerous inflammatory cells to form a strong neo-tissue construct. However, cells from the muscle fiber fragments did not survive in this milieu. Properties of the new neo-tissue construct must be evaluated at the time of full degradation of the scaffold before its possible clinical value in pelvic organ prolapse surgery can be evaluated.

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Section: Discussioncontrasting

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In these studies, we found that cells from the MFFs survived and participated in the regenerative process, significantly affecting some of the biomechanical properties. 4–6…”

Section: Introductionmentioning

confidence: 99%

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Examinations of a new long-term degradable electrospun polycaprolactone scaffold in three rat abdominal wall models

et al. 2017

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“…However, this approach requires extensive in vitro cell and tissue processing, which is costly and time‐consuming. An alternative option to address the current limitations has been previously tested via implantation of muscle fibers (MFs) and bundles into the muscle defect (Collins & Zammit, ; Gras, Klarskov, & Lose, ; Hall, Banks, Chamberlain, & Olwin, ; Jango, Gras, Christensen, & Lose, ; Lecoeur et al, ). Previous studies have utilized single or multiple MFs with random distributions in size and irregular fiber orientation/organization, which were obtained by a simple mincing or enzymatic digestion process of the muscle tissue.…”

Section: Introductionmentioning

confidence: 99%

Use of uniformly sized muscle fiber fragments for restoration of muscle tissue function

Yoo

Park

et al. 2019

J Tissue Eng Regen Med

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Treatment of extensive muscle loss due to traumatic injury, congenital defects, or tumor ablations is clinically challenging. The current treatment standard is grafting of autologous muscle flaps; however, significant donor site morbidity and graft tissue availability remain a problem. Alternatively, muscle fiber therapy has been attempted to treat muscle injury by transplanting single fibers into the defect site. However, irregularly organized long fibers resulted in low survivability due to delay in vascular and neural integration, thus limiting the therapeutic efficacy. Therefore, no effective method is available to permanently restore extensive muscle injuries. To address the current limitations, we developed a novel method that produces uniformly sized native muscle fiber fragments (MFFs) for muscle transplantation. We hypothesized that fragmentation of muscle fibers into small and uniformly sized fragments would allow for rapid reassembly and efficient engraftment within the defect site, resulting in accelerated recovery of muscle function. Our results demonstrate that the processed MFFs have a dimension of approximately 100 μm and contain living muscle cells on extracellular matrices. In preclinical animal studies using volumetric defect and urinary incontinence models, histological and functional analyses confirmed that the transplanted MFFs into the injury sites were able to effectively integrate with host muscle tissue, vascular, and neural systems, which resulted in significant improvement of muscle function and mass. These results indicate that the MFF technology platform is a promising therapeutic option for the restoration of muscle function and can be applied to various muscle defect and injury cases.

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Tissue-engineering with muscle fiber fragments improves the strength of a weak abdominal wall in rats

et al. 2016

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Muscle fragments on a scaffold in rats: a potential regenerative strategy in urogynecology

Cited by 9 publications

References 22 publications

Examinations of a new long-term degradable electrospun polycaprolactone scaffold in three rat abdominal wall models

Examinations of a new long-term degradable electrospun polycaprolactone scaffold in three rat abdominal wall models

Use of uniformly sized muscle fiber fragments for restoration of muscle tissue function

Tissue-engineering with muscle fiber fragments improves the strength of a weak abdominal wall in rats

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